A rapid procedure for the determination of 2-aminoisobutyric acid in enzalutamide bulk drug substance based on hydrophilic interaction chromatography with fluorescence detection was developed. Fluorescence detection after postcolumn derivatization with o-phthaldialdehyde/2-mercaptoethanol was carried out at excitation and emission wavelength of 345 nm and 450 nm, respectively. The postcolumn reaction conditions such as reaction temperature, mobile phase and derivatization reagent flow rate and the reagents concentrations were studied and optimized due to steric hindrance of amino group of 2-aminoisobutyric acid. The derivatization reaction was applied for the hydrophilic interaction chromatography method which was based on COSMOSIL HILIC column with a mobile phase consisting of a mixture of 25 mmol/L acetic acid adjusted to pH 5.5 (using 1 mol/L potassium hydroxide) and acetonitrile using an isocratic elution (28:72, ν/ν). The benefit of the reported approach consists in a simple sample pretreatment and a quick and sensitive hydrophilic interaction chromatography method. The developed method was validated in terms of linearity, limit of detection, limit of quantification, accuracy, precision and selectivity according to the International Conference on Harmonisation guidelines. The developed method was demonstrated to be applied for the analysis of 2-AIBA in routine quality control evaluation of commercial samples of enzalutamide bulk drug substance.
- MeSH
- acetonitrily chemie MeSH
- antagonisté androgenních receptorů analýza chemie MeSH
- fenylthiohydantoin analogy a deriváty analýza chemie MeSH
- fluorescenční spektrometrie přístrojové vybavení metody MeSH
- hydrofobní a hydrofilní interakce MeSH
- kontaminace léku prevence a kontrola MeSH
- kyseliny aminoisomáselné analýza MeSH
- limita detekce MeSH
- merkaptoethanol chemie MeSH
- o-ftalaldehyd chemie MeSH
- reprodukovatelnost výsledků MeSH
- řízení kvality * MeSH
- vysokoúčinná kapalinová chromatografie přístrojové vybavení metody MeSH
- Publikační typ
- časopisecké články MeSH
- validační studie MeSH
