The goal of the present study was to investigate the daily amount of motor activity and the daily rhythm of tryptophan and serotonin in goats housed in individual boxes under a 12/12 light/dark cycle. We equipped six Girgentana breed goats with Actiwatch-Mini® (Cambridge Neurotechnology Ltd, UK), actigraphy-based data loggers that record a digitally integrated measure of motor activity. Also blood samples were collected at four-hour intervals over a 48 h period via an intravenous cannula inserted into the jugular vein. The concentration of tryptophan (TRP) and serotonin (5-HT) were assessed by the high-performance liquid chromatography (HPLC) method. Our results outline a diurnal pattern of motor activity and levels of tryptophan in the serum, and a nocturnal pattern of levels of serotonin in the serum, which underline the impact of endogenous serotoninergic activity on the behavioural aspects of the circadian response to light.
The goal of the present study was to investigate the daily rhythms of calcium, phosphate and 1.25 dihydroxyvitamin D3 (1.25-(OH)2-D3) in the blood serum of goats. Blood samples from six Girgentana breed goats were collected via an intravenous cannula inserted into the jugular vein at four-hour intervals over a 48-hour period (starting at 08:00 hours on day 1 and finishing at 04:00 on day 2). The serum concentration of calcium and phosphate was measured by photometric test and of 1.25-(OH) 2-D3 by HPLC. All parameters were expressed as mean ? SEM. The one-way repeated measures analysis of variance (ANOVA) was used to determine significant differences. p values <0.05 were considered statistically significant. ANOVA showed a highly significant effect of time in all the parameters studied (p<0.0001 for calcium and phosphate and p<0.0002 for 1.25-(OH) 2-D3). The application of the periodic model and the statistical analysis of the Cosinor enabled us to define the periodic parameters and their acrophases (expressed in hours) during the 2 days of monitoring: all the studied parameters exhibited diurnal acrophases, which were within 12:44 and 17:28 hours. The results obtained led us to reveal the existence of a daily rhythm for the parameters considered and their temporal physiological values are useful for their implications in the formulation of therapeutic and nutritional protocols in the goat.
- MeSH
- 24,25-dihydroxyvitamin D3 analýza chemie krev MeSH
- cirkadiánní rytmus fyziologie MeSH
- fosforečnany vápenaté analýza chemie krev MeSH
- kozy fyziologie krev MeSH
- sérum fyziologie chemie MeSH
- spektrofotometrie infračervená metody využití MeSH
- vysokoúčinná kapalinová chromatografie metody využití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- MeSH
- klíště virologie MeSH
- kozy krev virologie MeSH
- neutralizující protilátky krev MeSH
- Orbivirus izolace a purifikace MeSH
- sérologické testy MeSH
- Geografické názvy
- Československo MeSH
