Recreational use of the potent synthetic opioid 3,4- dichloro-N-(2-(dimethylamino)cyclohexyl)-N-methylbenzamide (U-47700) is rising, accompanied by increasingly frequent cases of serious intoxication. This article reports a case of near-fatal U-47700 intoxication. A man was found unconscious (with drug powder residues). After 40 h in hospital (including 12 h of supported ventilation), he recovered and was discharged. Liquid chromatography/high-resolution mass spectrometry (LC/HRMS) or gas chromatography/mass spectrometry (GC/MS) were used to detect and quantify substances in powders, serum and urine. Powders contained U-47700 and two synthetic cannabinoids. Serum and urine were positive for U-47700 (351.0 ng/mL), citalopram (

• MeSH
• benzamidy škodlivé účinky   analýza   MeSH
• benzodiazepiny analýza   MeSH
• chromatografie kapalinová MeSH
• citalopram analýza   MeSH
• dospělí MeSH
• hmotnostní spektrometrie MeSH
• lidé MeSH
• midazolam analýza   MeSH
• odhalování abúzu drog MeSH
• plynová chromatografie s hmotnostně spektrometrickou detekcí MeSH
• předávkování léky * MeSH
• soudní toxikologie MeSH
• zakázané drogy škodlivé účinky   analýza   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• kazuistiky MeSH
• Geografické názvy
• Česká republika MeSH

A new, simple, rapid, sensitive, and repeatable isocratic reverse-phase HPLC method was developed and validated for simultaneous determination of midazolam and its main three hydroxylated metabolites, i.e. 1'-hydroxymidazolam, 4-hydroxymidazolam, and 1',4-dihydroxymidazolam in rat liver perfusate and also plasma. Diazepam was used as an internal standard to ensure precision and accuracy of this method. Analytes were extracted from alkalinized samples into diethyl ether using single-step liquid-liquid extraction. A C18 analytical column and a mobile phase composed of acetonitrile and sodium acetate buffer were used for the chromatographic separation with UV detection. Limits of detection varied between 7.9 and 19.6 microg/L for midazolam and its hydroxy metabolites. The overall recovery for the analytes exceeded 92%, for concentrations twice the limits of detection. The intra- and inter-day precision at three different concentrations never exceeded 8 and 11% variation, respectively. This method is applicable for modeling and description of possible pharmacological interactions on rat (CYP3A1/2) or human (CYP3A4/5) cytochrome P450 enzymes.

• MeSH
• financování organizované MeSH
• hypnotika a sedativa analýza   krev   MeSH
• krysa rodu rattus MeSH
• midazolam analýza   krev   MeSH
• perfuze MeSH
• potkani Wistar MeSH
• referenční standardy MeSH
• reprodukovatelnost výsledků MeSH
• senzitivita a specificita MeSH
• spektrofotometrie ultrafialová MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH