Low-grade inflammation links obesity, insulin resistance, and cardiovascular diseases. We investigated the effects of laparoscopic sleeve gastrectomy (LSG) on expression profile of genes involved in inflammatory pathways in subcutaneous adipose tissue (SCAT) and peripheral monocytes (PM). At baseline, obese group had significantly increased mRNA expression of proinflammatory chemokines (CCL-3, -17, -22), chemokine receptor CCR1 and cytokines (IL-10, IL-18) in SCAT and chemokine and other proinflammatory receptors (CCR-1, -2, -3, TLR-2, -4) in PM relative to control group. LSG decreased body weight, improved metabolic profile and reduced mRNA expression of up-regulated chemokine receptors, chemokines and cytokines in SCAT. In contrast, expression profiles in PM were largely unaffected by LSG. We conclude that LSG improved proinflammatory profile in subcutaneous fat but not in peripheral monocytes. The sustained proinflammatory and chemotactic profile in PM even 2 years after LSG may contribute to partial persistence of metabolic complications in obese patients after metabolic surgery.
- MeSH
- chemokiny CC genetika metabolismus MeSH
- dospělí MeSH
- exprese genu * MeSH
- gastrektomie metody MeSH
- hmotnostní úbytek MeSH
- interleukin-10 genetika metabolismus MeSH
- interleukin-18 genetika metabolismus MeSH
- laparoskopie MeSH
- lidé středního věku MeSH
- lidé MeSH
- messenger RNA genetika metabolismus MeSH
- monocyty metabolismus patologie MeSH
- obezita genetika metabolismus patologie chirurgie MeSH
- orgánová specificita MeSH
- podkožní tuk metabolismus patologie MeSH
- receptory CCR genetika metabolismus MeSH
- stanovení celkové genové exprese MeSH
- toll-like receptor 2 genetika metabolismus MeSH
- toll-like receptor 4 genetika metabolismus MeSH
- zánět genetika metabolismus patologie chirurgie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
