- MeSH
- antigeny povrchové metabolismus MeSH
- glykokonjugáty chemie imunologie terapeutické užití MeSH
- imunoterapie MeSH
- kolorektální nádory imunologie terapie MeSH
- krysa rodu rattus MeSH
- lektiny chemie metabolismus MeSH
- melanom experimentální imunologie terapie MeSH
- myši MeSH
- receptory mitogenů metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- myši MeSH
- zvířata MeSH
Six lectins were investigated for their binding affinity to MCF-7 cells stimulated and non-stimulated by estradiol. Except UEA-7 and Con A, all lectins (PNA, DBA, RCA60, WGA) showed the prevalent binding affinity to cytoplasm of polygonal cells in the centre of colonies. These cells differentiated more rapidly in non-stimulated cultures and intensity of their staining depended on cultivation time. The UEA-1 showed a weak binding capability which indicates that L-fucose is not a terminal sugar in glycoconjugates. Some other findings suggest that terminal expression of L-fucose can be regulated by other steroids. Con A bound all cells and was independent on estradiol stimulation.
- MeSH
- buněčná diferenciace MeSH
- buněčné linie MeSH
- estradiol farmakologie MeSH
- histocytochemie MeSH
- lidé MeSH
- nádory prsu metabolismus patologie MeSH
- receptory mitogenů účinky léků metabolismus MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The effects of lactose, L-fucose, and D-glucose on differentiation and glycosylation of MCF-7 cells from human mammary cancer were tested. For determination of glycosylation, six lectins with various binding specificity were used. The cells cultivated in the medium with D-glucose alone showed the best growth. The cells growing in the medium with L-fucose displayed, at the beginning growth acceleration which was followed by a rapid onset of necrotization. A decreased content of D-glucose usually resulted in an accelerated differentiation and a more pronounced secretory activity of cells. Expression of binding sites for lectins PNA and WGA was higher in cultures with elevated proliferation and reduced differentiation, DBA bound better the cells cultivated in the presence of lactose, lectins Con A and RCA60 in the presence of L-fucose. The UEA-1 showed only minimal affinity for MCF-7 cells. The majority of lectins showed an increased binding ability to secretory vacuoles and vesicles in the presence of L-fucose. The results obtained support the hypothesis that cell differentiation is associated with various stages of glycosylation and that the type of sugar in the medium can significantly affect glycosylation and differentiation processes.
- MeSH
- buněčná diferenciace účinky léků MeSH
- buněčné linie MeSH
- fukosa farmakologie MeSH
- glukosa farmakologie MeSH
- glykosylace MeSH
- histocytochemie MeSH
- laktosa farmakologie MeSH
- lidé MeSH
- nádory prsu metabolismus patologie MeSH
- receptory mitogenů účinky léků metabolismus MeSH
- sacharidy farmakologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The panel of six lectins was used for demonstration of glycosylative changes in the hyperplastic, adenomatous and carcinomatous mucosa of large bowel. The obtained results were rather heterogeneous with regard to lectin binding capacity in the single cases but they proved a certain uniformity of changes inside every tested group. These changes cohered with decrease of capability dysplastic and neoplastic cells to produce of normal goblets and with loss of cell polarity. Possible causes of these alterations are discussed.
- MeSH
- glykosylace MeSH
- histocytochemie MeSH
- kolon metabolismus patologie MeSH
- lidé MeSH
- nádory tračníku metabolismus patologie MeSH
- receptory mitogenů metabolismus MeSH
- rektum metabolismus patologie MeSH
- střevní sliznice metabolismus patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
