Telomerase maintains the ends of eukaryotic chromosomes, and its activity is an important parameter correlating with the proliferative capacity of cells. We have investigated cell cycle-specific changes in telomerase activity using cultures of Desmodesmus quadricauda, a model alga naturally synchronized by light/dark entrainment. A quantitative telomerase assay revealed high activity in algal cultures, with slight changes during the light period. Significantly increased telomerase activity was observed at the end of the dark phase, when cell division was complete. In contrast to other models, a natural separation between nuclear and cellular division typical for the cell cycle in D. quadricauda made this observation possible.
- MeSH
- biotest MeSH
- buněčné dělení MeSH
- Chlorophyta cytologie enzymologie účinky záření MeSH
- fotoperioda MeSH
- kinetika MeSH
- kultivované buňky MeSH
- rostlinné proteiny izolace a purifikace metabolismus MeSH
- světlo MeSH
- telomerasa izolace a purifikace metabolismus MeSH
- telomery metabolismus účinky záření MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
