Extensive phytochemical analysis of the CHCl3-soluble part of an ethanolic extract of branches and twigs of Broussonetia papyrifera led to the isolation of fourteen compounds, including a novel 5,11-dioxabenzo[b]fluoren-10-one derivative named broussofluorenone C (12). The isolated compounds 1-14 were characterized based on their NMR and HRMS data, and examined for their anti-inflammatory activities in LPS-stimulated THP-1 cells as well as for their cellular antioxidant effects. Compounds 7-10 and 12 showed inhibitory effects on NF-κB/AP-1 activation and compounds 7-9 were subsequently confirmed to suppress the secretion of both IL-1β and TNF-α in LPS-stimulated THP-1 cells more significantly than the prednisone used as a positive control. In the CAA assay, compound 10 exhibited the greatest antioxidant effect, greater than that of the quercetin used as a positive control. The results show possible beneficial effects and utilization of B. papyrifera wood in the treatment of inflammatory diseases as well as oxidative stress.
- MeSH
- Anti-Inflammatory Agents chemistry isolation & purification pharmacology MeSH
- Antioxidants chemistry isolation & purification pharmacology MeSH
- Broussonetia chemistry MeSH
- Interleukin-1beta antagonists & inhibitors biosynthesis MeSH
- Cells, Cultured MeSH
- Humans MeSH
- Lipopolysaccharides antagonists & inhibitors pharmacology MeSH
- Molecular Structure MeSH
- NF-kappa B analysis antagonists & inhibitors biosynthesis MeSH
- Oxidative Stress drug effects MeSH
- THP-1 Cells MeSH
- Tumor Necrosis Factor-alpha antagonists & inhibitors biosynthesis MeSH
- Transcription Factor AP-1 analysis antagonists & inhibitors biosynthesis MeSH
- Cell Survival drug effects MeSH
- Dose-Response Relationship, Drug MeSH
- Structure-Activity Relationship MeSH
- Check Tag
- Humans MeSH
- Publication type
- Journal Article MeSH
- Research Support, Non-U.S. Gov't MeSH
