Electrical characteristics of living cells have been proven to reveal important details about their internal structure, charge distribution and composition changes in the cell membrane, as well as the extracellular context. An impedance flow cytometry is a common approach to determine the electrical properties of a cell, having the advantage of label-free and high throughput. However, the current techniques are complex and costly for the fabrication process. For that reason, we introduce an integrated dual microneedle-microchannel for single-cell detection and electrical properties extraction. The dual microneedles utilized a commercially available tungsten needle coated with parylene. When a single cell flows through the parallel-facing electrode configuration of the dual microneedle, the electrical impedance at multiple frequencies is measured. The impedance measurement demonstrated the differential of normal red blood cells (RBCs) with three different sizes of microbeads at low and high frequencies, 100 kHz and 2 MHz, respectively. An electrical equivalent circuit model (ECM) was used to determine the unique membrane capacitance of individual cells. The proposed technique demonstrated that the specific membrane capacitance of an RBC is 9.42 mF/m-2, with the regression coefficients, ρ at 0.9895. As a result, this device may potentially be used in developing countries for low-cost single-cell screening and detection.
- MeSH
- Cell Membrane MeSH
- Electric Impedance * MeSH
- Electric Capacitance MeSH
- Flow Cytometry methods MeSH
- Publication type
- Journal Article MeSH
... Structure of Ion Channels 39 -- The Nicotinic Acetylcholine Receptor 41 -- Physical Properties of the ... ... and Length Constant 116 -- Membrane Resistance and Longitudinal Resistance 116 -- Calculating Membrane ... ... Resistance and Internal Resis tance 117 -- Specific Resistance 117 -- The Effect of Diameter on Cable ... ... Characteristics 118 -- Membrane Capacitance 118 -- Time Constant 120 -- Capacitance in a Cable 121 - ... ... Structures and Pathways of the Brain C—1 -- Glossary G-1 -- Bibliography BB-1 -- Index I-1 ...
4th ed. XIX,580,A-8,B-5,C-8,G-7,BB-60,I-10 : obr.,tab.,grafy.
... -- Cathode follower output time constant -- Cathode follower input resistance -- Cathode follower input ... ... input capacitance -- 3. ... ... transformation -- Current transformation -- Capacitance transformation -- Photoelectric transformation ... ... Distribution of inter-spike intervals 632 -- H. ... ... Spreading depression in deep brain structures. ...
3. rev. ed. 824 s. : il.
... Homeostasis: A Framework for Human Physiology 1 -- Chemical Composition of the Body 20 -- Cellular Structure ... ... Proteins, and Metabolism 45 -- SECTION A SECTION В -- SECTION С -- SECTION D -- SECTION E -- Cell Structure ... ... Cell Membranes 96 -- 5 Control of Cells by Chemical Messengers 120 -- 6 Neuronal Signaling and the Structure ... ... SECTION A SECTION В -- SECTION С SECTION D -- Neural Tissue Membrane Potentials 145 Synapses 160 Structure ... ... INQUIRI ES -- 44 -- 3 Cellular Structure, Proteins, and Metabolism 45 section A Cell Structure 46 -- ...
13th edition xxiv, 707 s. : il., tab. ; 28 sm
- Conspectus
- Fyziologie člověka a srovnávací fyziologie
- NML Fields
- fyziologie
- NML Publication type
- kolektivní monografie
... Westbrook -- The Structural and Functional Blueprint of Neurons Is Similar to Epithelial Cells 69 -- ... ... Membranous Organelles Are Selectively Distributed Throughout the Neuron 69 -- The Cytoskeleton Determines ... ... Siegelbaum -- Input Resistance Determines the Magnitude of Passive Changes in Membrane Potential 140 ... ... -- Membrane Capacitance Prolongs the Time Course of Electrical Signals 142 -- Membrane and Axoplasmic ... ... Resistance Affect the Efficiency of Signal Conduction 143 -- Large Axons Are More Easily Excited Than ...
4th ed. xxxiii, 1414 s. : il., tab., grafy ; 30 cm
- MeSH
- Behavior MeSH
- Molecular Biology MeSH
- Nervous System Diseases MeSH
- Nervous System MeSH
- Neurochemistry MeSH
- Neurophysiology MeSH
- Neurons MeSH
- Neurosciences MeSH
- Publication type
- Monograph MeSH
- Conspectus
- Fyziologie člověka a srovnávací fyziologie
- NML Fields
- neurovědy
- biologie
