Widely used classical angiography with the use of iodine contrast agents is highly problematic, particularly in patients with diabetes mellitus, cardiac and pulmonary diseases, or degree III or IV renal insufficiency. Some patients may be susceptible to allergic reaction to the iodine contrast substance. The intravenous injection of a bolus of CO2 (negative contrast) is an alternative method, which is, however, currently only used for imaging blood vessels of the lower limbs. The aim of our project was to design and test on an animal model a methodology for injecting the CO2 foam which would minimize the possibility of embolization of the brain tissue and heart infarction, leading to their damage. This is important research for the further promotion of the use of CO2, which is increasingly important for endovascular diagnosis and treatment, because carbon-dioxide-related complications are extremely rare. CO2 foam was prepared by the rapid mixing in a 2:1 ratio of CO2 and fetal bovine serum (FBS)-enriched Dulbecco's Modified Eagle Medium (DMEM). Freshly prepared CO2 foam was administered into the catheterized rat tail vein or cannulated rat abdominal aorta and inferior vena cava (IVC). CO2 foam was compared with commercially available microbubbles (lipid shell/gas core). The rat heart in its parasternal long axis was imaged in B-Mode and Non-linear Contrast Mode before/during and after the contrast administration. Samples of the brain, heart and lungs were collected and subjected to histological examination. The non-linear contrast imaging method enables the imaging of micron-sized gas microbubbles inside a rat heart. The significantly shorter lifetime of the prepared CO2 foam is a benefit for avoiding the local ischemia of tissues.

• MeSH
• angiografie MeSH
• jod * MeSH
• kontrastní látky MeSH
• krysa rodu rattus MeSH
• mikrobubliny MeSH
• oxid uhličitý * škodlivé účinky   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu rattus MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH

Background: Skeletal muscle contains populations of multipotent adult stem cells also referred to as muscle–derivedstem cells.Aim: The main goal of this study was to isolate and culture human adult stem cells from skeletal muscle and characterizethem.Methods: Muscle–derived stem cells were isolated from biopsy specimens of femoral muscle. The cells werecultured in Dulbecco's modifi ed Eagle's minimal essential medium supplemented with 10% fetal calf serum and gentamycin.When they reached confl uence, they were sub–passaged up to the third passage. Cells from the last passagewere prepared for TEM analysis. Production of ?–actin and desmin was confi rmed by histochemistry. Moreover, thephenotypic characterization was performed.Results: Primary isolated muscle–derived stem cells had a fi broblast–like shape. During subsequent passages theymaintained this morphology. TEM analysis showed typical ultrastructural morphology of mesenchymal stem cells.They had large pale nuclei with a large amount of euchromatine. Nuclei were irregular with noticeable nucleoli. Dilatedcisterns of rough endoplasmic reticulum were present in cytoplasm. In certain parts of the cytoplasm there were aggregatesof granules of glycogen. The products of cells were actively secreted into the extracellular matrix. They expressed?–actin and desmin. The results of phenotypic characterization showed that almost all analyzed cells were CD13,CD34, CD56 positive and CD45 negative. Moreover, they did not express anti–human fi broblast surface protein.Conclusions: Muscle–derived stem cells exhibited typical characteristics typical for mesenchymal stem cells. Afteranalysis of their diff erentiation potential they could be used in tissue engineering and regenerative medicine.

• MeSH
• biopsie metody   využití   MeSH
• dediferenciace buněk MeSH
• financování organizované MeSH
• fluorescenční protilátková technika metody   využití   MeSH
• gentamiciny MeSH
• imunohistochemie metody   využití   MeSH
• kultivační techniky metody   využití   MeSH
• lidé MeSH
• mezenchymální kmenové buňky cytologie   MeSH
• multipotentní kmenové buňky cytologie   MeSH
• regenerativní lékařství metody   trendy   MeSH
• svalové buňky účinky léků   MeSH
• tkáňové inženýrství metody   trendy   MeSH
• Check Tag
• lidé MeSH

• MeSH
• vzteklina diagnóza   MeSH
• Publikační typ
• laboratorní příručky MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• infekční lékařství
• chemie, klinická chemie
• NLK Publikační typ
• publikace WHO