Neospora caninum, Toxoplasma gondii and Encephalitozoon cuniculi are important pathogens with affinity to the central nervous system of many animals. 240 brains of wild carnivores were examined by PCR-based diagnosis. The presence of N. caninum DNA was confirmed in 4.61% (7/152) red foxes (Vulpes vulpes). DNA of T. gondii was found in 4.92% (3/61) martens (Martes sp.) and in 1.32% (2/152) red foxes. DNA of E. cuniculi was determined in 3.28% (2/61) martens and in one examined European otter (Lutra lutra). There were no co-infections found. These results provide the first evidence of E. cuniculi in the European otter, the first report of N. caninum in foxes in the Czech Republic and confirm the presence of T. gondii in wild carnivores in the Czech Republic.

• MeSH
• Carnivora parazitologie   MeSH
• divoká zvířata parazitologie   MeSH
• DNA fungální analýza   MeSH
• Encephalitozoon cuniculi izolace a purifikace   MeSH
• encephalitozoonóza diagnóza   epidemiologie   veterinární   MeSH
• financování organizované MeSH
• kokcidióza diagnóza   epidemiologie   veterinární   MeSH
• mozek parazitologie   MeSH
• Neospora izolace a purifikace   MeSH
• polymerázová řetězová reakce metody   veterinární   MeSH
• prevalence MeSH
• protozoální DNA analýza   MeSH
• Toxoplasma izolace a purifikace   MeSH
• toxoplazmóza zvířat diagnóza   epidemiologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Geografické názvy
• Česká republika MeSH

Toxoplasma gondii is a zoonotic parasite with a world-wide distribution. House mice (Mus musculus) play an important role as a reservoir host in the parasite life cycle. However, their detection in mouse brain is limited because the host potentially harbours only a few tissue cysts. In order to improve the diagnosis, we tested a novel protocol for T. gondii detection in mice and compared this technique to a standard PCR-based protocol using a commercial kit for DNA isolation. Efficacy of magnetic capture for isolation of T. gondii DNA from whole host brains was tested in brain samples of laboratory mice spiked with 1 up to 10(4) tachyzoites. Real-time PCR revealed that even 1-5 tachyzoites can be detected after magnetic capture. Also this method is suitable to quantify parasite numbers in mouse brains with more than 10 tachyzoite equivalents. To assess the two techniques in wild mice, we employed a dataset consisting of 243 individuals. The prevalence of T. gondii detected by magnetic capture and qPCR and by commercial isolation and PCR was 1.2% and 0%, respectively. The magnetic capture and quantitative PCR seems to be a highly sensitive and specific diagnostic method for both laboratory research and wild population surveys.

• MeSH
• genetické techniky normy   MeSH
• limita detekce MeSH
• magnetické jevy * MeSH
• myši MeSH
• polymerázová řetězová reakce normy   MeSH
• protozoální DNA analýza   izolace a purifikace   MeSH
• Toxoplasma genetika   MeSH
• toxoplazmóza zvířat diagnóza   MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH

Coprological examination of 40 Apodemus mystacinus Danford and Alston 1877 from Jordan revealed oocysts of three species of genus Eimeria. Two species are described as new. Eimeria zuhairamri sp. n. has broadly ellipsoidal oocysts 29.6 (27.0-34.0) x 23.3 (22.0-25.0) mum with distinctly granulated wall and oocyst residuum. Endogenous development occurs in jejunum and ileum. Eimeria alorani sp. n. has oocysts 26.9 (23.0-29.0) x 19.3 (18.0-22.0) mum with smooth wall and absent residuum. Endogenous development is confined to the caecum. The third species, developing in jejunum, has oocysts morphologically indistinguishable from Eimeria uptoni. The identity of E. uptoni and the taxonomy of Eimeria of Apodemus are discussed.

• MeSH
• cékum parazitologie   MeSH
• divoká zvířata parazitologie   MeSH
• Eimeria izolace a purifikace   klasifikace   růst a vývoj   MeSH
• feces parazitologie   MeSH
• financování organizované MeSH
• ileum parazitologie   MeSH
• jejunum parazitologie   MeSH
• kokcidióza parazitologie   veterinární   MeSH
• mikrofotografie MeSH
• Murinae parazitologie   MeSH
• nemoci hlodavců parazitologie   MeSH
• oocysty cytologie   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH