Subterranean clover is an important annual forage legume, whose diploidy and inbreeding nature make it an ideal model for genomic analysis in Trifolium. We reported a draft genome assembly of the subterranean clover TSUd_r1.1. Here we evaluate genome mapping on nanochannel arrays and generation of a transcriptome atlas across tissues to advance the assembly and gene annotation. Using a BioNano-based assembly spanning 512 Mb (93% genome coverage), we validated the draft assembly, anchored unplaced contigs and resolved misassemblies. Multiple contigs (264) from the draft assembly coalesced into 97 super-scaffolds (43% of genome). Sequences longer than >1 Mb increased from 40 to 189 Mb giving 1.4-fold increase in N50 with total genome in pseudomolecules improved from 73 to 80%. The advanced assembly was re-annotated using transcriptome atlas data to contain 31 272 protein-coding genes capturing >96% of the gene content. Functional characterization and GO enrichment confirmed gene expression for response to water deprivation, flavonoid biosynthesis and embryo development ending in seed dormancy, reflecting adaptation to the harsh Mediterranean environment. Comparative analyses across Papilionoideae identified 24 893 Trifolium-specific and 6325 subterranean-clover-specific genes that could be mined further for traits such as geocarpy and grazing tolerance. Eight key traits, including persistence, improved livestock health by isoflavonoid production in addition to important agro-morphological traits, were fine-mapped on the high-density SNP linkage map anchored to the assembly. This new genomic information is crucial to identify loci governing traits allowing marker-assisted breeding, comparative mapping and identification of tissue-specific gene promoters for biotechnological improvement of forage legumes.

• MeSH
• genom rostlinný genetika   MeSH
• genomika metody   MeSH
• sekvenční analýza DNA metody   MeSH
• Trifolium genetika   MeSH
• Publikační typ
• časopisecké články MeSH

"Now widely adopted on courses throughout the world, the prestigious Nutrition Society Textbook series provides students with the scientific basics in nutrition in the context of a systems and disease approach rather than on a nutrient by nutrient basis. In addition books provide a means to enable teachers and students to explore the core principles of nutrition and to apply these throughout their training to foster critical thinking at all times.This NS Textbook on Sport and Exercise Nutrition has been written to cover the latest information on the science and practice of sport and exercise nutrition. A key concept behind this textbook is that it aims to combine the viewpoints of world leading nutrition experts from both academia/research and a practical stand point. Plus where necessary there are additional practitioner based authors to ensure theory is translated into practice for each chapter in the form of either 'practice tips' or 'information sheets' at the end of relevant chapters.The textbook in essence can be divided into three distinct but integrated parts:Part 1: covers the key components of the science that supports the practice of sport and exercise nutrition including comprehensive reviews on: nutrients both in general and as exercise fuels; exercise physiology; hydration, micronutrients; and supplements. Part 2: moves into focusing on specific nutrition strategies to support different types of training including: resistance; power/sprint; middle distance/speed endurance; endurance; technical/skill, team; and specific competition nutrition needs.The unique format of this textbook is that it breaks down nutrition support into training specific as opposed to the traditional sport specific support. This reflects the majority of current sport and exercise requirements of the need to undertake concurrent training and therefore facilitating targeted nutrition support to the different training components through the various macro and micro training cycles.Part 3: explores some of the practical issues encountered in working in the sport and exercise nutrition field and includes key sport related topics such as: disability sport; weight management; eating disorders; bone and gut health; immunity; injury; travel; and special populations and situations.READERSHIP: Students of nutrition and dietetics at both undergraduate and postgraduate level. All those working in the field of nutrition and related health sciences"--Provided by publisher.

• MeSH
• cvičení * fyziologie   MeSH
• fyziologie výživy MeSH
• nutriční vědy MeSH
• sporty * fyziologie   MeSH
• tělesná výkonnost fyziologie   MeSH
• Publikační typ
• monografie MeSH

• Konspekt
• Fyziologie člověka a srovnávací fyziologie
• Hygiena. Lidské zdraví
• NLK Obory
• nutriční terapie, dietoterapie a výživa
• tělovýchovné lékařství
• tělovýchovné lékařství

• MeSH
• dítě MeSH
• hematologické nádory MeSH
• kojenec MeSH
• novorozenec MeSH
• Check Tag
• dítě MeSH
• kojenec MeSH
• novorozenec MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• hematologie a transfuzní lékařství
• onkologie
• pediatrie
• NLK Publikační typ
• kolektivní monografie

• MeSH
• fagocyty fyziologie   MeSH
• monocyty MeSH
• mononukleární fagocytární systém fyziologie   MeSH
• výzkum MeSH
• výzkumné techniky MeSH

• Konspekt
• Buněčná biologie. Cytologie
• NLK Obory
• cytologie, klinická cytologie
• NLK Publikační typ
• kolektivní monografie

• MeSH
• kmenové buňky MeSH
• transplantace kmenových buněk MeSH
• transplantace kostní dřeně MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• hematologie a transfuzní lékařství
• NLK Publikační typ
• kolektivní monografie

• MeSH
• farmakoterapie klasifikace   metody   MeSH
• nemoc MeSH
• patologické procesy farmakoterapie   klasifikace   MeSH

• Konspekt
• Farmacie. Farmakologie
• NLK Obory
• farmakoterapie
• farmacie a farmakologie
• NLK Publikační typ
• kolektivní monografie

• MeSH
• infekční nemoci MeSH
• Publikační typ
• periodika MeSH

• Konspekt
• Patologie. Klinická medicína
• NLK Obory
• infekční lékařství