Transplantation of male germ line cells into sterilized recipients has been used in mammals for conventional breeding as well as for transgenesis. We have previously adapted this approach for the domestic chicken and we present now an improvement of the germ cell transplantation technique by using an enriched subpopulation of c-Kit-positive spermatogonia as donor cells. Dispersed c-Kit positive testicular cells from 16 to 17 week-old pubertal donors were transplanted by injection directly into the testes of recipient males sterilized by repeated gamma irradiation. We describe the repopulation of the recipient's testes with c-Kit positive donor testicular cells, which resulted in the production of functional heterologous spermatozoa. Using manual semen collection, the first sperm production in the recipient males was observed about nine weeks after the transplantation. The full reproduction cycle was accomplished by artificial insemination of hens and hatching of chickens.

• MeSH
• kur domácí genetika   metabolismus   fyziologie   MeSH
• protoonkogenní proteiny c-kit metabolismus   MeSH
• spermatogeneze fyziologie   MeSH
• spermatogonie cytologie   MeSH
• sterilizace reprodukční MeSH
• testis cytologie   účinky záření   MeSH
• transplantace buněk metody   MeSH
• umělá inseminace MeSH
• zvířata MeSH
• Check Tag
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• adenosintrifosfát MeSH
• metabolismus lipidů MeSH
• metabolismus sacharidů MeSH
• metabolismus MeSH
• popálení MeSH
• proteiny metabolismus   MeSH