Motivation: Sanger sequencing is still being employed for sequence variant detection by many laboratories, especially in a clinical setting. However, chromatogram interpretation often requires manual inspection and in some cases, considerable expertise. Results: We present GLASS, a web-based Sanger sequence trace viewer, editor, aligner and variant caller, built to assist with the assessment of variations in 'curated' or user-provided genes. Critically, it produces a standardized variant output as recommended by the Human Genome Variation Society. Availability and implementation: GLASS is freely available at http://bat.infspire.org/genomepd/glass/ with source code at https://github.com/infspiredBAT/GLASS. Contact: nikos.darzentas@gmail.com or malcikova.jitka@fnbrno.cz. Supplementary information: Supplementary data are available at Bioinformatics online.

• MeSH
• alternativní sestřih MeSH
• genotypizační techniky metody   MeSH
• lidé MeSH
• nádorový supresorový protein p53 genetika   MeSH
• polymorfismus genetický MeSH
• sekvenční analýza DNA metody   MeSH
• sekvenční analýza RNA metody   MeSH
• software * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH