The impact of carnivore parvovirus infection on wild populations is not yet understood; disease signs are mainly developed in pups and assessing the health of litters in wild carnivores has big limitations. This study aims to shed light on the virus dynamics among wild carnivores thanks to the analysis of 213 samples collected between 1994 and 2013 in wild ecosystems from Spain. We determined the presence of carnivore parvovirus DNA by real-time PCR and sequenced the vp2 gen from 22 positive samples to characterize the strains and to perform phylogenetic analysis. The presence of carnivore parvovirus DNA was confirmed in 18% of the samples, with a higher prevalence detected in wolves (Canis lupus signatus, 70%). Fourteen sequences belonging to nine wolves, three Eurasian badgers (Meles meles), a common genet (Genetta genetta) and a European wildcat (Felis silvestris) were classified as canine parvovirus 2c (CPV-2c); five sequences from three wolves, a red fox (Vulpes vulpes) and a stone marten (Martes foina) as CPV-2b; and three sequences from a badger, a genet and a stone marten as feline parvovirus (FPV). This was the first report of a wildcat infected with a canine strain. Sequences described in this study were identical or very close related to others previously found in domestic carnivores from distant countries, suggesting that cross-species transmission takes place and that the parvovirus epidemiology in Spain, as elsewhere, could be influenced by global factors.

• MeSH
• Carnivora virologie   MeSH
• divoká zvířata MeSH
• fylogeneze MeSH
• hospodářská zvířata MeSH
• hostitelská specificita MeSH
• infekce viry z čeledi Parvoviridae epidemiologie   veterinární   virologie   MeSH
• kočky MeSH
• kvantitativní polymerázová řetězová reakce veterinární   MeSH
• lišky MeSH
• Mustelidae MeSH
• parvovirus psů genetika   MeSH
• Parvovirus genetika   izolace a purifikace   MeSH
• psi MeSH
• virus infekční panleukopenie koček genetika   MeSH
• vlci MeSH
• zeměpis MeSH
• zvířata MeSH
• Check Tag
• kočky MeSH
• psi MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Španělsko MeSH

Tetraparvovirus, formerly known as Partetravirus, is a newly discovered genus in the family Parvoviridae that is considered phylogenetically distinct from other parvoviruses. However, nothing is known about the prevalence of Tetraparvovirus in special livestock living on the Qinghai-Tibet Plateau of China, such as Tibetan pigs and Tibetan sheep. A pair of special primers was designed based on the conserved regions in the genome of ungulate tetraparvovirus 2 (P-PARV4) and ungulate tetraparvovirus 4 (O-PARV4) and was used to detect P-PARV4 in domestic pigs and Tibetan pigs and O-PARV4 in ovines and Tibetan sheep. The results showed a 15.59 and 9.38% prevalence of P-PARV4 in domestic pigs (18.96% in Gansu Province and 11.76% in Qinghai Province) and Tibetan pigs (14.28% in Gansu Province and 4.44% in Qinghai Province), respectively, and a 7.31 and 5.20% prevalence of O-PARV4 in ovines (6.61% in Gansu Province and 8.00% in Qinghai Province) and Tibetan sheep (4.55% in Gansu Province and 5.50% in Qinghai Province), respectively. The prevalence of P-PARV4 was 14.76% (31/210) for ≤1-month-old pigs and 10.58% (20/189) for > 1-month-old pigs, and the positive rates of O-PARV4 were 7.65% (18/235) for ≤1-month-old sheep and 5.05% (11/218) for > 1-month-old sheep. The phylogenetic analysis of NS1, VP1, VP2 and the whole PARV4-related provirus genome demonstrated that both P-PARV4 and O-PARV4 sequences in this study were more closely related to the sequences of other strains discovered in the same genus of animals. The identity analyses for the full-length VP2 genomes of O-PARV4 revealed 98.84-100.00% sequence identity among the 7 strains and the previously reported strain, which was 98.60-99.28% for P-PARV4. In the present study, for the first time, we have provided comprehensive information regarding the widespread infection of P-PARV4 and O-PARV4 in special livestock on the Qinghai-Tibet Plateau in China. Our present findings highlight the importance of epidemiologic surveillance to limit the spread of Tetraparvovirus in livestock at high altitudes in China.

• MeSH
• dobytek virologie   MeSH
• fylogeneze MeSH
• genom virový * MeSH
• infekce viry z čeledi Parvoviridae epidemiologie   veterinární   MeSH
• ovce virologie   MeSH
• Parvovirinae genetika   izolace a purifikace   MeSH
• polymerázová řetězová reakce MeSH
• prasata virologie   MeSH
• prevalence MeSH
• sekvenční analýza DNA MeSH
• virové proteiny genetika   MeSH
• zvířata MeSH
• Check Tag
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Čína MeSH
• Tibet MeSH

Laboratorní diagnóza infekce parvovirem B 19 je nejčastěji založena na průkazu specifických IgM a sérokonverze IgG. V práci, která navazuje na předchozí studie řešené v rámci grantu GA CR 313/93/0872: Studium epidemiologického a klinického významu parvoviru B 19 v České republice (6,8), jsou uvedeny některé anamnestické údaje (věk, měsíc onemocnění a den výsevu exantému) a sérologické nálezy u 34 nemocných ze 4 oblastí ČR s megaerythema infectiosum i potvrzené v Referenční laboratori pro zarděnky, spalničky a parotitidu SZU koncem roku 1996 a v průběhu 1. pololetí 1997. Sérologické potvrzení infekce parvovirem B 19 bylo provedeno imunoenzymatickými soupravami MRL Diagnostics (California, USA), založenými na bázi standardní metody Engvala a Perlmanna (3).

The laboratory diagnosis of infection with parvovirus is most frequently based on evidence of specific IgM and seroconversion of IgG. In the presented paper which is a continuation of the previous investigation elaborated within the framework of the grant GA313/93/0872: Investigation of the epidemiological and clinical importance of parvovirus B 19 in the Czech Republic (6,8), the authors present some anamnestic data (age, month when disease was contracted and day of eruption of the rash) and serological findings in 34 patients from 4 areas in the Czech Republic with megaerythema infectiosum confirmed in the reference laboratory for German measles, measles, and parotitis of the NIPH at the end of 1996 and first half of 1997. For serological confirmation of infection by parvovirus B 19 immunoenzymatic kits MRL Diagnosistics (California, USA) were used based on the Engval and Perlmann's standard method (3).

• MeSH
• dítě MeSH
• dospělí MeSH
• ELISA MeSH
• infekce viry z čeledi Parvoviridae diagnóza   epidemiologie   MeSH
• infekční nemoci MeSH
• klinické laboratorní techniky metody   MeSH
• lidé MeSH
• lidský parvovirus B19 patogenita   MeSH
• mladiství MeSH
• těhotenství MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH
• Geografické názvy
• Česká republika MeSH

• MeSH
• dítě MeSH
• erythema infectiosum MeSH
• infekce viry z čeledi Parvoviridae epidemiologie   MeSH
• lidé MeSH
• lidský parvovirus B19 MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Geografické názvy
• Česká republika MeSH

• MeSH
• anemie etiologie   terapie   MeSH
• artralgie etiologie   terapie   MeSH
• artritida etiologie   terapie   MeSH
• dítě MeSH
• dospělí MeSH
• erythema infectiosum etiologie   terapie   MeSH
• hydrops fetalis etiologie   terapie   MeSH
• infekce viry z čeledi Parvoviridae diagnóza   epidemiologie   MeSH
• lidé MeSH
• lidský parvovirus B19 genetika   patogenita   MeSH
• těhotenství MeSH
• Check Tag
• dítě MeSH
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• těhotenství MeSH
• ženské pohlaví MeSH