Authentic standards of food flavonoids are important for human metabolic studies. Their isolation from biological materials is impracticable; however, they can be prepared in vitro. Twelve sulfated metabolites of luteolin, myricetin, and ampelopsin were obtained with arylsulfotransferase from Desulfitobacterium hafniense and fully characterized by high-performance liquid chromatography, MS, and NMR. The compounds were tested for their ability to scavenge 1,1-diphenyl-2-picrylhydrazyl, 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid), and N,N-dimethyl-p-phenylenediamine radicals, to reduce ferric ions and Folin-Ciocalteu reagent, and to inhibit tert-butyl hydroperoxide-induced lipid peroxidation of rat liver microsomes. The activity differed considerably even between monosulfate isomers. The parent compounds and myricetin-3'-O-sulfate were the most active while other compounds displayed significantly lower activity, particularly luteolin sulfates. No mutagenic activity of the parent compounds and their main metabolites was observed; only myricetin showed minor pro-mutagenicity. The prepared sulfated metabolites are now available as authentic standards for future in vitro and in vivo metabolic studies.
- MeSH
- antioxidancia chemie farmakologie MeSH
- arylsulfotransferasa chemie MeSH
- bakteriální proteiny chemie MeSH
- biofyzikální jevy MeSH
- biokatalýza MeSH
- Desulfitobacterium enzymologie MeSH
- flavonoidy chemie metabolismus farmakologie MeSH
- isomerie MeSH
- jaterní mikrozomy účinky léků metabolismus MeSH
- krysa rodu rattus MeSH
- luteolin chemie metabolismus farmakologie MeSH
- molekulární struktura MeSH
- peroxidace lipidů účinky léků MeSH
- sírany chemie metabolismus MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
ISCO using activated sodium persulphate is a widely used technology for treating chlorinated solvent source zones. In sensitive areas, however, high groundwater sulphate concentrations following treatment may be a drawback. In situ biogeochemical transformation, a technology that degrades contaminants via reduced iron minerals formed by microbial activity, offers a potential solution for such sites, the bioreduction of sulphate and production of iron sulphides that abiotically degrade chlorinated ethenes acting as a secondary technology following ISCO. This study assesses this approach in the field using hydrochemical and molecular tools, solid phase analysis and geochemical modelling. Following a neutralisation and bioaugmentation, favourable conditions for iron- and sulphate-reducers were created, resulting in a remarkable increase in their relative abundance. The abundance of dechlorinating bacteria (Dehalococcoides mccartyi, Dehalobacter sp. and Desulfitobacterium spp.) remained low throughout this process. The activity of iron- and sulphate-reducers was further stimulated through application of magnetite plus starch and microiron plus starch, resulting in an increase in ferrous iron concentration (from
- MeSH
- chemické látky znečišťující vodu analýza metabolismus MeSH
- chlor metabolismus MeSH
- Chloroflexi metabolismus MeSH
- čištění vody metody MeSH
- Desulfitobacterium metabolismus MeSH
- ethyleny metabolismus MeSH
- halogenace MeSH
- oxidace-redukce MeSH
- Peptococcaceae metabolismus MeSH
- podzemní voda analýza chemie mikrobiologie MeSH
- regenerace a remediace životního prostředí metody MeSH
- rozpouštědla metabolismus MeSH
- sírany metabolismus MeSH
- sloučeniny sodíku MeSH
- tetrachlorethylen analýza metabolismus MeSH
- trichlorethylen analýza metabolismus MeSH
- železo metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
Potential metabolites of bioactive compounds are important for their biological activities and as authentic standards for metabolic studies. The phenolic compounds contained in olive oil are an important part of the human diet, and therefore their potential metabolites are of utmost interest. We developed a convenient, scalable, one-pot chemoenzymatic method using the arylsulfotransferase from Desulfitobacterium hafniense for the sulfation of the natural olive oil phenols tyrosol, hydroxytyrosol, and of their monoacetylated derivatives. Respective monosulfated (tentative) metabolites were fully structurally characterized using LC-MS, NMR, and HRMS. In addition, Folin-Ciocalteu reduction, 1,1-diphenyl-2-picrylhydrazyl radical scavenging, and antilipoperoxidant activity in rat liver microsomes damaged by tert-butylhydroperoxide were measured and compared to the parent compounds. As expected, the sulfation diminished the radical scavenging properties of the prepared compounds. These compounds will serve as authentic standards of phase II metabolites.
- MeSH
- acetylace MeSH
- arylsulfotransferasa chemie MeSH
- bakteriální proteiny chemie MeSH
- biokatalýza MeSH
- Desulfitobacterium enzymologie MeSH
- fenethylalkohol analogy a deriváty chemická syntéza chemie MeSH
- fenoly chemie MeSH
- hmotnostní spektrometrie MeSH
- molekulární struktura MeSH
- olivový olej chemie MeSH
- scavengery volných radikálů chemická syntéza chemie MeSH
- sírany chemie MeSH
- vysokoúčinná kapalinová chromatografie MeSH
- Publikační typ
- časopisecké články MeSH
Sulfated quercetin derivatives are important authentic standards for metabolic studies. Quercetin-3'-O-sulfate, quercetin-4'-O-sulfate, and quercetin-3-O-sulfate as well as quercetin-di-O-sulfate mixture (quercetin-7,3'-di-O-sulfate, quercetin-7,4'-di-O-sulfate, and quercetin-3',4'-di-O-sulfate) were synthetized by arylsulfotransferase from Desulfitobacterium hafniense. Purified monosulfates and disulfates were fully characterized using MS and NMR and tested for their 1,1-diphenyl-2-picrylhydrazyl (DPPH), 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS⁺) and N,N-dimethyl-p-phenylenediamine (DMPD) radical scavenging, Folin-Ciocalteau reduction (FCR), ferric reducing antioxidant power (FRAP), and anti-lipoperoxidant activities in rat liver microsomes damaged by tert-butylhydroperoxide. Although, as expected, the sulfated metabolites were usually less active than quercetin, they remained still effective antiradical and reducing agents. Quercetin-3'-O-sulfate was more efficient than quercetin-4'-O-sulfate in DPPH and FCR assays. In contrast, quercetin-4'-O-sulfate was the best ferric reductant and lipoperoxidation inhibitor. The capacity to scavenge ABTS+• and DMPD was comparable for all substances, except for disulfates, which were the most efficient. Quantum calculations and molecular dynamics simulations on membrane models supported rationalization of free radical scavenging and lipid peroxidation inhibition. These results clearly showed that individual metabolites of food bioactives can markedly differ in their biological activity. Therefore, a systematic and thorough investigation of all bioavailable metabolites with respect to native compounds is needed when evaluating food health benefits.
The Watson River drains a portion of the SW Greenland ice sheet, transporting microbial communities from subglacial environments to a delta at the head of Søndre Strømfjord. This study investigates the potential activity and community shifts of glacial microbiota deposited and buried under layers of sediments within the river delta. A long-term (12-month) incubation experiment was established using Watson River delta sediment under anaerobic conditions, with and without CO2/H2enrichment. Within CO2/H2-amended incubations, sulphate depletion and a shift in the microbial community to a 52% predominance of Desulfosporosinus meridiei by day 371 provides evidence for sulphate reduction. We found evidence of methanogenesis in CO2/H2-amended incubations within the first 5 months, with production rates of ~4 pmol g-1 d-1, which was likely performed by methanogenic Methanomicrobiales- and Methanosarcinales-related organisms. Later, a reduction in methane was observed to be paired with the depletion of sulphate, and we hypothesise that sulphate reduction out competed hydrogenotrophic methanogenesis. The structure and diversity of the original CO2/H2-amended incubation communities changed dramatically with a major shift in predominant community members and a decline in diversity and cell abundance. These results highlight the need for further investigations into the fate of subglacial microbiota within downstream environments.
- MeSH
- geologické sedimenty mikrobiologie MeSH
- ledový příkrov mikrobiologie MeSH
- methan MeSH
- Methanomicrobiales MeSH
- Methanosarcinales MeSH
- mikrobiota * MeSH
- Peptococcaceae MeSH
- řeky mikrobiologie MeSH
- sírany MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Grónsko MeSH
Cíl Stanovit prevalenci jednotlivých anaerobních bakterií u pacientů s chronickou ránou. Materiál a metodika Do souboru byli zařazeni všichni pacienti hospitalizovaní s chronickou ránou na Klinice popálenin a rekonstrukční chirurgie FN Brno v období od 1. 1. 2008 do 31. 12. 2010. U těchto pacientů byl pravidelným a cíleným mikrobiologickým odběrem získán materiál z kožních defektů. V diagnostice anaerobních původců infekce chronického defektu má nezastupitelnou roli stěr na sterilní vatový tampón následně zanořený do transportního média, které umožňuje přežití anaerobních bakterií. Tato metoda je preferována před otiskem. Po transportu do mikrobiologické laboratoře se materiál vyočkuje na kultivační půdy včetně VL agaru pro růst anaerobních bakterií. Kultivace anaerobních mikrobů vyžaduje anaerobní prostředí a časový interval 48 hodin. Následná identifikace anaerobů se provádí pomocí sestavy diagnostických disků a diagnostických souprav Remel a API bioMérieux. Výsledky Celkem bylo do skupiny zařazeno 95 pacientů (32 žen) s pozitivní izolací anaerobní bakterie v chronickém defektu, kteří byli hospitalizovaní ve sledovaném období. 75 pacientů v souboru (78,95 %) bylo hospitalizováno se vstupní diagnózou dekubitu, 13 pacientů (13,68 %) s bércovým vředem a 7 pacientů (7,36 %) s diabetickým defektem dolní končetiny (diabetickou nohou). M:F ratio 2,97:1. U těchto pacientů bylo izolováno celkem 198 kmenů anaerobních bakterií. Nejčastějším anaerobem izolovaným z chronické rány je Bacteroides fragilis celkem 115 kmenů (58,08 %), dále Peptococcus sp. 20 kmenů (10,10 %) a Peptostreptococcus sp. 19 kmenů (9,60 %) a Prevotela melaninogenica 14 kmenů (7,07 %). Závěr Ve studii se nám podařilo stanovit prevalenci základních anaerobních bakterií u pacientů s chronickou ránou. Potvrdili jsme také skutečnost, že nejvyšší výskyt anaerobů je u pacientů s dekubity.
Aim To establish the prevalence of particular anaerobic bacteria in patients with chronic wound. Material and methodology All patients, hospitalized with a chronic wound in the Clinic of burns and reconstructive surgery of Faculty Hospital of Brno in the period from January 1st, 2008 till December 31st, 2010, were enrolled in our data set. In these patients, the material was collected by regular and targeted collection from skin defects. A swab made with a sterile cotton wool swab subsequently dipped into transport medium, which enables a survival of anaerobic bacteria, plays an insubstitutable role in a diagnostic procedure of anaerobic etiological agents of chronic defect infections. This method is preferred to an imprinting. Following the transport to microbiologic laboratory, the material is inoculated in culture media including VL agar for a growth of anaerobic bacteria. The cultivation of anaerobic germs demands an anaerobic environment and a time period of 48 hours. The subsequent identification of anaerobes is performed by a set of diagnostic discs and diagnostic kits Remel and API bioMérieux. Results In total, 95 patients (32 women) with positive isolation of anaerobic bacteria in a chronic defect, who were hospitalized in the monitored period, were enrolled in our group. 75 patients in the group (78.95 %) were hospitalized with an initial diagnosis of the pressure ulcer, 13 patients (13.68%) with the crural ulcer and 7 patients (7.36 %) with a diabetic defect of lower extremity(the diabetic foot). M:F ratio 2.97:1. There were a total of 198 anaerobic bacteria tribes isolated in these patients. The most common anaerobic bacteria isolated from a chronic wound is Bacteroides fragilis 115 tribes in total (58.08 %), followed by Peptococcus sp. 20 tribes (10.10 %), Peptostreptococcus sp. 19 tribes (9.60 %), and Prevotela melaninogenica 14 tribes (7.07 %). Conclusion In our study we established the prevalence of basic anaerobic bacteria in patients with chronic wound. We also proved the fact that the highest incidence of anaerobes is in patients with pressure ulcers.
- MeSH
- anaerobní bakterie izolace a purifikace MeSH
- Bacteroides fragilis izolace a purifikace MeSH
- bércové vředy mikrobiologie MeSH
- chronická nemoc MeSH
- dekubity mikrobiologie MeSH
- diabetická noha mikrobiologie MeSH
- hojení ran MeSH
- infekce v ráně mikrobiologie MeSH
- kultivační techniky MeSH
- lidé MeSH
- pacienti hospitalizovaní MeSH
- Peptococcus izolace a purifikace MeSH
- Peptostreptococcus izolace a purifikace MeSH
- Prevotella melaninogenica izolace a purifikace MeSH
- retrospektivní studie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
The effects of 50 Hz magnetic fields on sulphate- reducing bacteria viability were studied electrochemically. Two types of graphite electrodes (pyrolytic and glassy carbon) covered with whole bacterial cells behind a dialysis membrane were used for electrochemical measurements. We found about 15% decrease of reduction peak current density (which indicates desulphurization activity of the bacterial cells - their metabolic activity) on cyclic voltammograms after magnetic field exposure compared to the control samples. We suppose that the magnetic field does not influence the metabolic activity (desulphurization) of sulphate-reducing bacteria but most probably causes bacterial death.
- MeSH
- aktinomykóza MeSH
- chybná diagnóza MeSH
- diferenciální diagnóza MeSH
- dospělí MeSH
- infekční nemoci kůže diagnóza farmakoterapie mikrobiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- Peptococcus MeSH
- Peptostreptococcus MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
