Alterations of hydrogen peroxide (H2O2) levels have a profound impact on numerous signaling cascades orchestrating plant growth, development, and stress signaling, including programmed cell death. To expand the repertoire of known molecular mechanisms implicated in H2O2 signaling, we performed a forward chemical screen to identify small molecules that could alleviate the photorespiratory-induced cell death phenotype of Arabidopsisthaliana mutants lacking H2O2-scavenging capacity by peroxisomal catalase2. Here, we report the characterization of pakerine, an m-sulfamoyl benzamide from the sulfonamide family. Pakerine alleviates the cell death phenotype of cat2 mutants exposed to photorespiration-promoting conditions and delays dark-induced senescence in wild-type Arabidopsis leaves. By using a combination of transcriptomics, metabolomics, and affinity purification, we identified abnormal inflorescence meristem 1 (AIM1) as a putative protein target of pakerine. AIM1 is a 3-hydroxyacyl-CoA dehydrogenase involved in fatty acid β-oxidation that contributes to jasmonic acid (JA) and salicylic acid (SA) biosynthesis. Whereas intact JA biosynthesis was not required for pakerine bioactivity, our results point toward a role for β-oxidation-dependent SA production in the execution of H2O2-mediated cell death.

• MeSH
• Arabidopsis cytologie   účinky léků   genetika   metabolismus   MeSH
• buněčná smrt účinky léků   MeSH
• buněčné dýchání účinky léků   genetika   MeSH
• cyklopentany metabolismus   MeSH
• fotosyntéza účinky léků   genetika   MeSH
• fyziologický stres MeSH
• hydroponie metody   MeSH
• kyselina salicylová metabolismus   MeSH
• listy rostlin cytologie   účinky léků   metabolismus   MeSH
• meristém cytologie   účinky léků   metabolismus   MeSH
• multienzymové komplexy genetika   metabolismus   MeSH
• oxylipiny metabolismus   MeSH
• peroxid vodíku antagonisté a inhibitory   farmakologie   MeSH
• proteiny huseníčku genetika   metabolismus   MeSH
• regulace genové exprese u rostlin * MeSH
• rostlinné buňky účinky léků   metabolismus   MeSH
• semena rostlinná účinky léků   MeSH
• signální transdukce MeSH
• stanovení celkové genové exprese MeSH
• sulfonamidy chemická syntéza   farmakologie   MeSH
• transkriptom MeSH
• výpočetní biologie metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity. The tested compounds can be screened within 6-9 weeks in contrast to the 6 months for traditionally used explants on solid medium. The method enables large-scale screening of potential antivirals for in vitro elimination of viruses from vegetatively propagated crops and ornamentals.

• MeSH
• acyklické uhlovodíky farmakologie   terapeutické užití   MeSH
• antivirové látky farmakologie   terapeutické užití   MeSH
• Brassica účinky léků   růst a vývoj   virologie   MeSH
• hydroponie metody   MeSH
• kultivační média MeSH
• mikrobiální testy citlivosti metody   MeSH
• nemoci rostlin terapie   virologie   MeSH
• nukleosidy farmakologie   terapeutické užití   MeSH
• replikace viru účinky léků   MeSH
• ribavirin farmakologie   terapeutické užití   MeSH
• Tymovirus účinky léků   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

• MeSH
• finanční podpora výzkumu jako téma MeSH
• hydroponie metody   MeSH
• oves metabolismus   MeSH
• titan aplikace a dávkování   MeSH
• Publikační typ
• kongresy MeSH