We recently completed the Special Issue on 'Plant Virus Epidemiology and Control'. As editors, we decided not to offer vouchers to scientists that submit to this issue. This action had an effect on the number of papers received and accepted. We received a total of 19 papers and we accepted four [...].
- MeSH
- epidemiologie MeSH
- nemoci rostlin prevence a kontrola terapie virologie MeSH
- rostlinné viry * MeSH
- Publikační typ
- úvodní články MeSH
- úvodníky MeSH
A new method was developed for testing antiviral compounds against plant viruses based on rapidly growing brassicas in vitro on liquid medium. This method enables exchange of media containing tested chemicals in various concentrations and simultaneous evaluation of their phytotoxicity and antiviral activity. While using ribavirin as a standard for comparison, phytotoxicity and ability of the acyclic nucleotide analogues (R)-PMPA, PMEA, PMEDAP, and (S)-HPMPC to eliminate ssRNA Turnip yellow mosaic virus (TYMV) were evaluated by this method. Double antibody sandwich ELISA and real-time PCR were used for relative quantification of viral protein and nucleic acid in plants. Ribavirin had the most powerful antiviral effect against TYMV. On the other hand, (R)-PMPA and PMEA had no antiviral effect and almost no phytotoxicity compared to the control. (S)-HPMPC and PMEDAP showed moderate antiviral effect, accompanied by higher phytotoxicity. The tested compounds can be screened within 6-9 weeks in contrast to the 6 months for traditionally used explants on solid medium. The method enables large-scale screening of potential antivirals for in vitro elimination of viruses from vegetatively propagated crops and ornamentals.
- MeSH
- acyklické uhlovodíky farmakologie terapeutické užití MeSH
- antivirové látky farmakologie terapeutické užití MeSH
- Brassica účinky léků růst a vývoj virologie MeSH
- hydroponie metody MeSH
- kultivační média MeSH
- mikrobiální testy citlivosti metody MeSH
- nemoci rostlin terapie virologie MeSH
- nukleosidy farmakologie terapeutické užití MeSH
- replikace viru účinky léků MeSH
- ribavirin farmakologie terapeutické užití MeSH
- Tymovirus účinky léků MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
