Background and objectives: Atherosclerosis is the underlying pathology of ischemic heart disease. There are many aggravating metabolic and oxidant parameters which are participating together overwhelming the pathology of vascular stenosis. Adipokines play a positive metabolic effect in healthy individuals and oxidation reaction greatly impacts the lipid metabolism and might negatively impact the condition. In the present study, we aimed to characterize the level of adiponectin, obestatin, and redox parameters in atherosclerotic patients.Methods: Serum was collected from atherosclerotic patients and froze to be ready for analysis.Results: The results indicated that hyperlipidemia significantly reduced adiponectin, obestatin, and antioxidant enzymes (catalase, glutathione, glutathione-S-transferase, and glutathione peroxidase) together with a significant increase in oxidant byproduct (malondialdehyde) and modulated lipid parameters (cholesterol, triglyceride, and high-density lipoprotein).Conclusion: The study concluded that atherosclerosis is associated with reduced antioxidant enzymes, obestatin, and adiponectin levels and increased lipid levels. These parameters play a great role in the patho-logical status of coronary stenosis.
- MeSH
- adiponektin krev metabolismus MeSH
- antioxidancia analýza metabolismus MeSH
- ateroskleróza etiologie patofyziologie MeSH
- ELISA MeSH
- ghrelin krev metabolismus MeSH
- hyperlipidemie * diagnóza etiologie patofyziologie MeSH
- koronární stenóza patofyziologie MeSH
- lidé MeSH
- malondialdehyd krev metabolismus MeSH
- metabolismus lipidů MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- klinická studie MeSH
The main health care challenges associated with diabetic patients are glycemic control. Insulin defection has been regarded as the mainstay which needs to be tackled to avoid glucose over presence in the circulatory system. These challenges have always been conjoined with the patient's redox status, hence, oxidants/antioxidants determine the fate of pancreatic tissue status and they are reciprocally interrelated. Various remedies have been utilized by patients themselves and healthcare workers to control hyperglycemia if any. Herbal and pharmacological therapy were always being used hand in hand. Herein, we are demonstrating the antioxidant effect of propolis and its role in modulation of lipid profile in type 2 diabetic patients using vitamin E for comparison in sequential mode i.e. vitamin E used for 8 weeks followed one-week washout period and then propolis therapy started in the same group of patients (n = 45). Thereby a sample of serum has been collected in the first visit (baseline and vitamin E started, followed by collecting serum after 8 weeks (second visit); followed by commencing of propolis after a washout week from the second visit, at the third visit another serum sample collected from all patients. Serum was analyzed for oxidant/antioxidant status represented by malondialdehyde (MDA) and total antioxidant status (TAS). Additionally, lipid profile has been measured from the same samples. The results indicate that both propolis and vitamin E positively modulated the measured parameters with superiority of propolis over vitamin E in improving these measured biomolecules. To conclude, propolis is an overall safe natural product and is inducing such positive effects in the diabetic patient, we do advise these patients to start propolis therapy as an adjuvant medication to control these deleterious biomolecules.
- MeSH
- biochemická analýza krve metody přístrojové vybavení MeSH
- diabetes mellitus 2. typu * farmakoterapie krev MeSH
- kombinace léků sitagliptin a metformin aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipidy krev MeSH
- malondialdehyd krev MeSH
- oxidační stres účinky léků MeSH
- propolis aplikace a dávkování farmakologie MeSH
- statistika jako téma MeSH
- vitamin E aplikace a dávkování farmakologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- klinická studie MeSH
- Geografické názvy
- Irák MeSH
Souvislosti a cíle: Záměrem této studie je objasnit vztah mezi úrovní oxidačního stresu (OS), depresí (D) a rizikem opakovaného výskytu stabilní ischemické choroby srdeční (sICHS). Metody: Retrospektivní studie se zúčastnilo 174 osob ve věku 45+: 86 hospitalizovaných pacientů kardiologického oddělení s rekurentní sICHS a 88 hospitalizovaných pacientů kardiologického oddělení s primární sICHS. Vážnost symptomů deprese byla hodnocena za použití 30bodového dotazníku Geriatrické škály deprese (GDS), platné lotyšské verze GDS-LAT. Každému pacientovi byly odebrány vzorky krve za účelem změření parametrů oxidačního stresu malondialdehydu (MDA) a glutathionu peroxidázy (GPx). Výsledky: 83,9 % vzorků mělo vysokou koncentraci MDA. V 72,4 % byl vzorek hodnoty GPx normální, v 17,8 % byla hodnota vysoká a v 9,8 % nízká. O něco více než polovina pacientů má depresi (44,3 % mírnou D a 6,9 % těžkou D). GPx se statisticky lišila u primární a rekurentní sICHS (p = 0,003). Pacienti s D a vysokým GPx měli 10,6krát vyšší pravděpodobnost opakované sICHS ve srovnání s pacienty bez D a s normální GPx (p = pacienti s D měli obě hodnoty MDA a GPx vysoké častěji než účastníci bez D, ale nebylo to statisticky významné [p = 0,51]). Závěr: V uvedené studii bylo zjištěno, že koncentrace antioxidačního (AO) enzymu GPx byla podstatně vyšší u depresivních pacientů s opakovanou sICHS ve srovnání s pacienty bez D a s pacienty s primární ICHS a pacienti s D i s vysokým GPx měli vyšší pravděpodobnost opakované sICHS ve srovnání s těmi bez D a s normálním GPx. Dá se předpokládat, že GPx je významnější marker rizika D a opakované sICHS. Koncentrace MDA ve většině z obou skupin (primární a opakovaná sICHS) pacientů by mohla dokazovat, že zvýšená koncentrace OS je všeobecně rizikový faktor ischemické choroby srdeční. Monitorování biomarkerů OS se zdá být významné ve zvládání komorbidity sICHS s D. Bylo by vhodné provést další studie, které by potvrdily tyto nálezy.
Aim: The aim of the study was to investigate the relationships between a level of oxidative stress (OS), depression (D) and risk of recurrence of stable coronary heart disease (SCHD). Methods: A retrospective study was conducted on 174 participants, at the age 45+ years: 86 in-patients of the cardiology department with a recurrent SCHD and 88 in-patients of the cardiology department with primary SCHD. The severity of depressive symptoms was assessed using the long 30-item form of Geriatric Depression Scale (GDS), valid Latvian version of GDS-LAT. The blood samples were taken from each patient to measure oxidative stress parameters malondialdehyde (MDA) and glutathione peroxidase (GPx). Results: 83.9% of the sample had high level of MDA. In 72.4% of the sample the GPx level was normal, in 17.8% it was high and in 9.8% low. Slightly more than a half of the patients were experiencing depression (44.3% - mild D and 6.9% - severe D). GPx was found statistically differing between primary and recurrent SCHD (p = 0,003). Patients with both D and high GPx had 10.6 times higher chances of recurrent SHCD compared to those without D and normal GPx (p = patients with present D were experiencing both - high levels of MDA and GPx - more often than responders with no D, but this wasn't statistically signifi cant [p = 0.51]). Conclusion: In the present study it was found that level of antioxidant (AO) enzyme GPx was signifi cantly higher in depressed patients with recurrent SCHD compared to patients without D and to patients with primary SCHD and patients with both D and high GPx had higher chances of recurrent SCHD compared to those without D and normal GPx. It could be supposed that GPx is a more signifi cant marker of risk of D and recurrence of SCHD. The high level of MDA in most of both (primary and recurrent SCHD) groups patients could evidence that increased OS is a risk factor for CHD in general. Monitoring OS biomarkers seems to be important in the management of SCHD comorbidity with D. Further studies are warranted to confi rm these findings.
- MeSH
- biologické markery MeSH
- deprese MeSH
- glutathionperoxidasa krev MeSH
- hematologické testy MeSH
- koronární nemoc * etiologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- malondialdehyd krev MeSH
- oxidační stres MeSH
- psychiatrické posuzovací škály MeSH
- recidiva MeSH
- retrospektivní studie MeSH
- rizikové faktory kardiovaskulárních chorob * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- spektrofotometrie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Publikační typ
- klinická studie MeSH
- práce podpořená grantem MeSH
Beside the key inhibition of acetylcholinesterase (AChE), involvement of oxidative stress in organophosphate (OP)-induced toxicity has been supported by experimental and human studies. On the other hand, according to our best knowledge, possible antioxidant properties of oximes, the only causal antidotes to OP-inhibited AChE, have been examined only by a few studies. Thus, we have determined the effect of four conventional (obidoxime, trimedoxime, pralidoxime, asoxime) and two promising experimental oximes (K027, K203) on dichlorvos (DDVP)-induced oxidative changes in vivo. Wistar rats (5/group) were treated with oxime (5% LD50 i.m) immediately after DDVP challenge (75% LD50 s.c). Oxidative stress biomarkers were determined in plasma and brain 60 min after the treatment: prooxidative-superoxide anion (O2·-) and total oxidative status (TOS); antioxidative-superoxide dismutase (SOD), total thiol (SH) groups, total antioxidant status (TAS) and paraoxonase (PON1); tissue oxidative stress burden-prooxidative-antioxidative balance (PAB) and oxidative stress index (OSI); oxidative tissue damage-malondialdehyde (MDA) and advanced oxidation protein products (AOPP). All oximes were able to attenuate DDVP-induced oxidative stress in rat plasma and brain. Changes of determined parameters in brain were not as prominent as it was seen in plasma. Based on OSI, better abilities of oxime K027, K203 and obidoxime to maintain DDVP-induced oxidative stress in rat brain were shown as compared to trimedoxime, pralidoxime and asoxime. Oximes can influence the complex in vivo redox processes that might contribute to their overall therapeutic efficacy. Further research is needed to understand the underlying molecular mechanisms involved in this phenomenon.
- MeSH
- aryldialkylfosfatasa krev MeSH
- biologické markery krev MeSH
- cholinesterasové inhibitory farmakologie MeSH
- dichlorvos toxicita MeSH
- krysa rodu rattus MeSH
- malondialdehyd krev MeSH
- mozek účinky léků MeSH
- obidoxim chlorid farmakologie MeSH
- otrava organofosfáty farmakoterapie MeSH
- oxidační stres účinky léků MeSH
- oximy farmakologie MeSH
- pralidoximové sloučeniny MeSH
- pyridinové sloučeniny farmakologie MeSH
- superoxiddismutasa krev MeSH
- trimedoxim farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
BACKGROUND: Topiramate (TPM) decreases cytokine release and generation of reactive oxygen species (ROS). Cytokine and endothelin-1 (ET-1) secretion and ROS formation play an important role in ischemia-reperfusion (I/R) injury. We aimed to evaluate whether TPM prevents damage occurring in lung tissue during I/R. MATERIALS AND METHODS: A total of 27 Wistar albino rats were divided into three groups of nine. To the I/R group, two hours of ischemia via infrarenal abdominal aorta cross-ligation and then two hours of reperfusion process were applied. TPM (100 mg/kg/day) orally for seven days was administered in the TPM treatment group. After the last dose of TPM treatment, respectively, two hours of ischemia and two hours of reperfusion were applied in this group. RESULTS: Tumor necrosis factor-alpha (TNF-α) (p < 0.05), malondialdehyde (MDA) (p < 0.05), myeloperoxidase (MPO) (p < 0.05) and ET-1 (p < 0.05) levels of TPM treatment group's lung tissue were significantly lower than for the I/R group. Caspase-3 and histopathological damage were rather lower than that of the I/R group. CONCLUSIONS: During I/R, lung damage occurs due to excessive TNF-α and ET-1 release and ROS generation. TPM could well reduce development of lung damage by decreasing cytokine and ET-1 release and levels of ROS produced.
- MeSH
- aorta abdominalis MeSH
- biologické markery krev MeSH
- kaspasa 3 krev MeSH
- krysa rodu rattus MeSH
- ligace MeSH
- malondialdehyd krev MeSH
- peroxidasa krev MeSH
- poškození plic farmakoterapie MeSH
- potkani Wistar MeSH
- reperfuzní poškození farmakoterapie MeSH
- TNF-alfa krev MeSH
- topiramat farmakologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu rattus MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
Double balloon enteroscopy (DBE) was introduced 15 years ago. The complications of diagnostic DBE are rare, acute pancreatitis is most redoubtable one (incidence about 0.3%). Hyperamylasemia after DBE seems to be a rather common condition respectively. The most probable cause seems to be a mechanical straining of the pancreas. We tried to identify patients in a higher risk of acute pancreatitis after DBE. We investigated several laboratory markers before and after DBE (serum cathepsin B, lactoferrin, E-selectin, SPINK 1, procalcitonin, S100 proteins, alfa-1-antitrypsin, hs-CRP, malondialdehyde, serum and urine amylase and serum lipase). Serum amylase and lipase rose significantly with the maximum 4 hours after DBE. Serum cathepsin and procalcitonin decreased significantly 4 hours after DBE compared to healthy controls and patients values before DBE. Either serum amylase or lipase 4 hours after DBE did not correlate with any markers before DBE. There was a trend for an association between the number of push-and-pull cycles and procalcitonin and urine amylase 4 hours after DBE; between procalcitonin and alfa-1-antitrypsin, cathepsin and hs-CRP; and between E-selectin and malondialdehyde 4 hours after DBE. We found no laboratory markers determinative in advance those patients in a higher risk of acute pancreatitis after DBE.
- MeSH
- akutní nemoc MeSH
- alfa-1-antitrypsin krev MeSH
- amylasy krev moč MeSH
- biologické markery krev moč MeSH
- C-reaktivní protein metabolismus MeSH
- dvojbalonová enteroskopie škodlivé účinky MeSH
- E-selektin krev MeSH
- hyperamylazemie krev etiologie MeSH
- kalcitonin krev MeSH
- kathepsiny krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipasa krev MeSH
- malondialdehyd krev MeSH
- pankreatitida krev etiologie MeSH
- rizikové faktory MeSH
- studie případů a kontrol MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
In coronary heart disease, the treatment of significant stenosis by percutaneous coronary intervention (PCI) with stent implantation elicits local and systemic inflammatory responses. This study was aimed at evaluation of the dynamics of inflammatory response and elucidation of the relationship between the fatty acid profile of red blood cell (RBC) membranes or plasma phospholipids and inflammation after PCI. High-sensitivity C-reactive protein (hsCRP), interleukin-6 (IL-6), serum amyloid A (SAA), malondialdehyde (MDA) and the fatty acid profiles were determined in patients with advanced coronary artery disease undergoing PCI before, 24 h and 48 h after drug-eluting stent implantation (n=36). Patients after PCI exhibited a significant increase in studied markers (hsCRP, IL-6, SAA, MDA). Many significant associations were found between the increase of IL-6, resp. SAA and the amounts of n-6 polyunsaturated fatty acids (namely linoleic, dihomo-gamma-linolenic, docosatetraenoic and docosapentaenoic acid), resp. saturated fatty acids (pentadecanoic, stearic, nonadecanoic) in erythrocyte membranes. The magnitude of the inflammatory response to PCI is related to erythrocyte membrane fatty acid profile, which seems to be a better potential predictor of elevation of inflammatory markers after PCI than plasma phospholipids.
- MeSH
- biologické markery krev MeSH
- erytrocytární membrána chemie MeSH
- fosfolipidy krev MeSH
- koronární angioplastika MeSH
- lidé MeSH
- malondialdehyd krev MeSH
- mastné kyseliny krev MeSH
- nemoci koronárních tepen krev chirurgie MeSH
- nenasycené mastné kyseliny krev MeSH
- oxidační stres MeSH
- pooperační komplikace krev MeSH
- průřezové studie MeSH
- senioři MeSH
- stenty škodlivé účinky MeSH
- zánět krev etiologie MeSH
- Check Tag
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
The aim of the study was to evaluate short-term heart rate variability (HRV) as an index of cardiac autonomic control in rats with lipopolysaccharide (LPS)-induced endotoxemia. Animals were injected intraperitoneally with LPS (100 microg/kg b.w.) and control group with an equivalent volume of saline. ECG recordings were done before (base) and 60, 120, 180, 240 and 300 min after LPS or saline administration. HRV magnitude was quantified by time and frequency-domain analysis (mean RR interval, SDRR, RMSSD, spectral powers in low (LF) and high frequency (HF) bands. Heart tissue homogenates and plasma were analyzed to determine interleukin 6 (IL-6), tumor necrosis factor alpha (TNF-alpha) and oxidative stress level (TBARS). Administration of lipopolysaccharide was followed by continuous rise in colonic body temperature compared to saline-treated controls. Endotoxemia in rats was accompanied by significant decrease in HRV spectral activity in high-frequency range at maximal body temperature (logHFpower: 1.2+/-0.5 vs. 1.9+/-0.6 ms(2), P<0.01). Increased IL-6 was found in heart tissue homogenates of LPS rats (8.0+/-0.6 vs. 26.4+/-4.8 pg/ml, (P<0.05). In conclusions, reduced HRV in HF band may indicate a decreased parasympathetic activity in LPS-induced endotoxemia as basic characteristics of altered cardiac control during response to endotoxemia.
- MeSH
- autonomní nervový systém patofyziologie MeSH
- bradykardie chemicky indukované patofyziologie MeSH
- časové faktory MeSH
- endotoxemie krev chemicky indukované patofyziologie MeSH
- interleukin-6 krev MeSH
- lipopolysacharidy * MeSH
- malondialdehyd krev MeSH
- mediátory zánětu krev MeSH
- modely nemocí na zvířatech MeSH
- myokard metabolismus MeSH
- oxidační stres MeSH
- potkani Wistar MeSH
- srdce inervace MeSH
- srdeční frekvence * MeSH
- termoregulace MeSH
- TNF-alfa krev MeSH
- zánět krev chemicky indukované patofyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
UNLABELLED: Consumption of antioxidant-enriched diets is 1 method of addressing obesity, which is associated with chronic oxidative stress and changes in the activity/expression of various enzymes. In this study, we hypothesized that the modulation of antioxidant enzymes and redox status through a cranberry extract (CBE)-enriched diet would differ between obese and nonobese mice. The CBE used in this study was obtained from the American cranberry (Vaccinium macrocarpon, Ericaceae), a popular constituent of dietary supplements that is a particularly rich source of (poly)phenols and has strong antioxidant properties. The present study was designed to test and compare the in vivo effects of 28-day consumption of a CBE-enriched diet (2%) on the antioxidant status of nonobese mice and mice with monosodium glutamate-induced obesity. Plasma, erythrocytes, liver, and small intestine were studied concurrently to obtain more complex information. The specific activities, protein, and messenger RNA expression levels of antioxidant enzymes as well as the levels of malondialdehyde and thiol (SH) groups were analyzed. Cranberry extract treatment increased the SH group content in plasma and the glutathione S-transferase activity in the erythrocytes of the obese and nonobese mice. In addition, in the obese animals, the CBE treatment reduced the malondialdehyde content in erythrocytes and increased NAD(P)H: quinone oxidoreductase (liver) and catalase (erythrocytes and small intestine) activities. The elevation of hepatic NAD(P)H: quinone oxidoreductase activity was accompanied by an increase in the corresponding messenger RNA levels. The effects of CBE on the activity of antioxidant enzymes and redox status were more pronounced in the obese mice compared with the nonobese mice.
- MeSH
- antioxidancia aplikace a dávkování MeSH
- dieta MeSH
- erytrocyty chemie MeSH
- glutathiontransferasa krev MeSH
- játra enzymologie MeSH
- katalasa krev metabolismus MeSH
- malondialdehyd krev MeSH
- messenger RNA analýza MeSH
- myši MeSH
- NAD(P)H dehydrogenasa (chinon) genetika metabolismus MeSH
- obezita krev chemicky indukované enzymologie MeSH
- ovoce chemie MeSH
- oxidace-redukce MeSH
- rostlinné extrakty aplikace a dávkování MeSH
- sulfhydrylové sloučeniny krev MeSH
- tenké střevo enzymologie MeSH
- Vaccinium macrocarpon * MeSH
- zvířata MeSH
- Check Tag
- myši MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Uric acid is the final product of human purine metabolism. It was pointed out that this compound acts as an antioxidant and is able to react with reactive oxygen species forming allantoin. Therefore, the measurement of allantoin levels may be used for the determination of oxidative stress in humans. The aim of the study was to clarify the antioxidant effect of uric acid during intense exercise. Whole blood samples were obtained from a group of healthy subjects. Allantoin, uric acid, and malondialdehyde levels in plasma and erythrocytes were measured using a HPLC with UV/Vis detection. Statistical significant differences in allantoin and uric acid levels during short-term intense exercise were found. Immediately after intense exercise, the plasma allantoin levels increased on the average of 200 % in comparison to baseline. Plasma uric acid levels increased slowly, at an average of 20 %. On the other hand, there were no significant changes in plasma malondialdehyde. The results suggest that uric acid, important antioxidant, is probably oxidized by reactive oxygen species to allantoin. Therefore allantoin may be suitable candidate for a marker of acute oxidative stress.
- MeSH
- alantoin krev MeSH
- běh fyziologie MeSH
- biologické markery krev MeSH
- dospělí MeSH
- erytrocyty metabolismus MeSH
- hemoglobiny analýza MeSH
- kyselina mléčná krev MeSH
- kyselina močová krev MeSH
- lidé MeSH
- malondialdehyd krev MeSH
- mladý dospělý MeSH
- reaktivní formy kyslíku metabolismus MeSH
- zátěžový test MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
