Background: Lipocalin-2 (LCN2) is a protein that has been associated with skeletal muscle regeneration, but details regarding its role in Arthritis remain unclear. The aim of the current study was to investigate LCN2 levels of Arthritis patients and its relationship with oxidative and antioxidative factors.Methods: The study includes (125) blood samples of persons aged 20-65 years were divided into a control group (apparently healthy) consisting of 55 samples [31female, 24 males] and a Patient group consisting of 70 samples [37female, 33 males] who were attending the bone diseases consultation unit at the Ibn Sina Teaching Hospital in Mosul, Iraq. Venous blood samples (10 ml) were collected after overnight fasting. To conduct Clinical analyses: Serum LCN2 level was determined by ELISA, also Malonaldehyde, glutathione, vitamin E, vitamin C, peroxy nitrite, peroxidase, and aryl esterase were estimated.Results: The findings revealed a significant increase in the levels of LCN2 in Arthritis compared to the control group and there was a significant decrease in the concentration of vitamin C, glutathione, vitamin E and the activity of the arylesterase in serum of patients with arthritis compared with the control group. Also, a significant increase in the activity of peroxidase, concentration of peroxynitrite and malondialdehyde for patients than a control group.Conclusion: These findings imply that LCN2 may play a substantial role in iron-related oxidative stress damage in arthritis. Thus a therapeutic candidate target for treatment.
The aim of this study was to develop and validate methods for the determination of vitamins B2, B9, E and A in serum using liquid chromatography with mass spectrometry (MS) detection. Vitamin analysis was performed using an ultra performance liquid chromatography combined with tandem MS. The compounds were separated on a BEH C18 RP column (2.1 × 100 mm, 1.7 μm) using a gradient elution with an analysis time of 10 min. Sample preparation included protein precipitation with ethanol. The concentration range in human serum was as follows: riboflavin 5-1000 nmol/L, folic acid 2.5-250 nmol/L, α-tocopherol 0.5-100 μmol/L and all-trans-retinol 25-2500 nmol/L. Accuracy and precision were validated according to Food and Drug Administration guidelines, with coefficients of variation ranging from 3.1-11.7% and recoveries from 94.4-107.5%. Routine monitoring of the complex range of vitamins in bariatric medicine is still not common. This is despite the fact that patients are at risk for glitch deficits, especially of a neurological nature. An analytical method that allows for the complex measurement of both water-soluble and fat-soluble vitamins is important and necessary for the clinical monitoring of bariatric patients. The method we have described could benefit both clinical practice and nutritional research.
- MeSH
- alfa-tokoferol * krev MeSH
- bariatrická chirurgie MeSH
- chromatografie kapalinová metody MeSH
- kyselina listová * krev MeSH
- lidé MeSH
- limita detekce MeSH
- lineární modely MeSH
- reprodukovatelnost výsledků MeSH
- riboflavin * krev MeSH
- tandemová hmotnostní spektrometrie * metody MeSH
- vitamin A * krev MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
The approaches to matrix effects determination and reduction in ultra-high performance supercritical fluid chromatography with mass spectrometry detection have been evaluated in this study using different sample preparation methods and investigation of different calibration models. Five sample preparation methods, including protein precipitation, liquid-liquid extraction, supported liquid extraction, and solid phase extraction based on both "bind and elute" and "interferent removal" modes, were optimized with an emphasis on the matrix effects and recovery of 8 forms of vitamin E, including α-, β-, γ-, and δ-tocopherols and tocotrienols, from plasma. The matrix effect evaluation included the use and comparison of external and internal calibration using three models, i.e., least square with no transformation and no weighting (1/x0), with 1/x2 weighting, and with logarithmic transformation. The calibration model with logarithmic transformation provided the lowest %-errors and the best fits. Moreover, the type of the calibration model significantly affected not only the fit of the data but also the matrix effects when evaluating them based on the comparison of calibration curve slopes. Indeed, based on the used calibration model, the matrix effects calculated from calibration slopes ranged from +92% to - 72% for α-tocopherol and from -77% to +19% in the case of δ-tocotrienol. Thus, it was crucial to calculate the matrix effect by Matuszewski's post-extraction approach at six concentration levels. Indeed, a strong concentration dependence was observed for all optimized sample preparation methods, even if the stable isotopically labelled internal standards (SIL-IS) were used for compensation. The significant differences between individual concentration levels and compounds were observed, even when the tested calibration range covered only one order of magnitude. In methods with wider calibration ranges, the inappropriate use of calibration slope comparison instead of the post-extraction addition approach could result in false negative results of matrix effects. In the selected example of vitamin E, solid-phase extraction was the least affected by matrix effects when used in interferent removal mode, but supported liquid extraction resulted in the highest recoveries. We showed that the calibration model, the use of a SIL-IS, and the analyte concentration level played a crucial role in the matrix effects. Moreover, the matrix effects can significantly differ for compounds with similar physicochemical properties and close retention times. Thus, in all bioanalytical applications, where different analytes are typically determined in one analytical run, it is necessary to carefully select the data processing in addition to the method for the sample preparation, SIL-IS, and chromatography.
- MeSH
- extrakce na pevné fázi metody MeSH
- hmotnostní spektrometrie * metody MeSH
- kalibrace MeSH
- lidé MeSH
- superkritická fluidní chromatografie * metody MeSH
- vitamin E * krev analýza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
OBJECTIVES: Concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios predict prognosis and the need for oxygen therapy in patients hospitalized for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection. The aims of the present study were to evaluate the changes of these biomarkers early in the course of infection, the association with the prior coronavirus disease (COVID-19) vaccination and therapeutic administration of Anti-SARS-CoV-2 monoclonal antibodies, investigation of other potential biomarkers including neuropilin, 8-hydroxy-2-deoxyguanosine and 8-hydroxyguanosine in patients hospitalized with SARS-CoV-2 infection and an assessment of these biomarkers and vitamins A, E and D in patients with post-COVID syndrome. METHODS: Urine and blood samples were obtained on the 1st to the 4th day and 4th to 7th day from 108 patients hospitalized with COVID-19. Chromatography tandem mass spectrometry methods were used to analyse neopterin, kynurenine, tryptophan, liposoluble vitamins, and DNA damage biomarkers. RESULTS: A statistically significant decrease of neopterin, kynurenine and kynurenine/tryptophan ratios was observed on after 4th to 7th day of hospitalization, and concentrations of these biomarkers were increased in patients with poor prognosis and subsequent post-COVID syndrome. The concentrations of remaining biomarker and vitamins were not associated with outcomes, although markedly decreased concentrations of vitamin A, E and D were noted. CONCLUSIONS: The concentrations of neopterin, kynurenine and kynurenine/tryptophan ratios decrease during the course of infection SARS-CoV-2 and are associated with the post-COVID syndrome. No other prognostic biomarkers were identified.
- MeSH
- biologické markery * krev MeSH
- COVID-19 * krev MeSH
- dospělí MeSH
- hospitalizace MeSH
- kynurenin * krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- neopterin * krev moč MeSH
- postakutní syndrom COVID-19 MeSH
- SARS-CoV-2 * izolace a purifikace MeSH
- senioři MeSH
- tryptofan * krev MeSH
- vitamin A krev MeSH
- vitamin D krev MeSH
- vitamin E krev MeSH
- vitaminy krev MeSH
- zánět krev MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
This article summarizes current information about the function, source, and bioavailability of fat-soluble vitamins A, D, E, and K. Furthermore, it shows dietary reference values stated by European Food Safety Authority for the adult population, lists hypovitaminosis high-risk groups and states potential complications that could arise from ingesting these compounds.
Článek podává shrnutí aktuálních informací o funkci, zdrojích a biologické dostupnosti liposolubilních vitaminů A, D, E a K. Dále uvádí výživové referenční hodnoty dle Evropského úřadu pro bezpečnost potravin pro dospělou populaci, důsledky, rizikové skupiny pro vznik hypovitaminóz a možné komplikace při vysokém příjmu těchto sloučenin.
- Klíčová slova
- hypervitaminóza,
- MeSH
- avitaminóza etiologie farmakoterapie patofyziologie MeSH
- lidé MeSH
- nedostatek vitaminu A etiologie farmakoterapie patofyziologie MeSH
- nedostatek vitaminu D etiologie farmakoterapie patofyziologie MeSH
- nedostatek vitaminu E etiologie farmakoterapie patofyziologie MeSH
- nedostatek vitaminu K etiologie farmakoterapie patofyziologie MeSH
- vitamin A fyziologie terapeutické užití MeSH
- vitamin D fyziologie terapeutické užití MeSH
- vitamin E fyziologie terapeutické užití MeSH
- vitamin K fyziologie terapeutické užití MeSH
- výživové doporučené dávky MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- přehledy MeSH
Vitamin E má u člověka jen relativně mírné projevy hypovitaminózy, které navíc nejsou, ve většině případů, způsobeny nedostatečným obsahem tokoferolů ve stravě. Pozornosti výživové politiky tak pravděpodobně uniká fakt, že skutečný příjem vitaminu E je u většiny populace (celosvětově) nedostatečný. Tento přehled shrnuje aktuální informace o doporučeném a reálnem příjmu vitaminu E a o rizicích a případných benefitech spojených s jeho nedostatečným či zvýšeným příjmem.
Vitamin E has only relatively mild manifestations of hypovitaminosis in humans, which, moreover, are not, in most cases, caused by insufficient content of tocopherols in the diet. Thus, the fact that the actual intake of vitamin E is (worldwide) insufficient in the majority of the population is likely to escape the attention of nutrition policy. This review summarizes current information on the recommended and actual intake of vitamin E and on the risks and possible benefits associated with insufficient or increased intake.
The main health care challenges associated with diabetic patients are glycemic control. Insulin defection has been regarded as the mainstay which needs to be tackled to avoid glucose over presence in the circulatory system. These challenges have always been conjoined with the patient's redox status, hence, oxidants/antioxidants determine the fate of pancreatic tissue status and they are reciprocally interrelated. Various remedies have been utilized by patients themselves and healthcare workers to control hyperglycemia if any. Herbal and pharmacological therapy were always being used hand in hand. Herein, we are demonstrating the antioxidant effect of propolis and its role in modulation of lipid profile in type 2 diabetic patients using vitamin E for comparison in sequential mode i.e. vitamin E used for 8 weeks followed one-week washout period and then propolis therapy started in the same group of patients (n = 45). Thereby a sample of serum has been collected in the first visit (baseline and vitamin E started, followed by collecting serum after 8 weeks (second visit); followed by commencing of propolis after a washout week from the second visit, at the third visit another serum sample collected from all patients. Serum was analyzed for oxidant/antioxidant status represented by malondialdehyde (MDA) and total antioxidant status (TAS). Additionally, lipid profile has been measured from the same samples. The results indicate that both propolis and vitamin E positively modulated the measured parameters with superiority of propolis over vitamin E in improving these measured biomolecules. To conclude, propolis is an overall safe natural product and is inducing such positive effects in the diabetic patient, we do advise these patients to start propolis therapy as an adjuvant medication to control these deleterious biomolecules.
- MeSH
- biochemická analýza krve metody přístrojové vybavení MeSH
- diabetes mellitus 2. typu * farmakoterapie krev MeSH
- kombinace léků sitagliptin a metformin aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- lipidy krev MeSH
- malondialdehyd krev MeSH
- oxidační stres účinky léků MeSH
- propolis aplikace a dávkování farmakologie MeSH
- statistika jako téma MeSH
- vitamin E aplikace a dávkování farmakologie MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- Publikační typ
- klinická studie MeSH
- Geografické názvy
- Irák MeSH
Metabolický syndrom (MetS) je symptomatický komplex charakterizovaný inzulinovou rezistencí, poruchou prooxidačně-antioxidační rovnováhy organismu s rozvojem subchronického zánětu a dyslipidemií. Cílem studie je prozkoumat vlivkomplexní farmaceutické kompozice (CPC) (antioxidanty a metabolitotropní látky), která se v lékařské praxi na Ukrajině široce používá jako multivitaminový komplex, na experimentální metabolický syndrom u potkanů. Byl hodnocen vliv CPC na korekci experimentálního MetS u potkanů, vyvolaného vysokým obsahem sacharidů a tuků ve stravě. MetS u potkanů byl charakterizován snížením citlivosti buněk na inzulin, zvýšeným obsahem glukózy a porušením její utilizace, a prooxidačně-antioxidační dysbalancí. Výsledky provedených studií naznačují pozitivní vliv CPC, který obsahuje etylestery omega-3 kyselin, vitamin E, koenzym Q10, zinek, vitamin A, biotin a selen, na citlivost buněk k inzulinu, utilizaci glukózy, trvání hyperglykémie a ukazatele oxidačních procesů volných radikálů a antioxidačníchobranných systémů u potkanů s experimentálním MetS. Tyto výsledky dokazují možnost využití CPC k korekci metabolického syndromu.
Metabolic syndrome (MetS) is a symptomatic complex characterized by insulin resistance, impaired prooxidant-antioxidantbalance of the body with the development of subchronic inflammation, and dyslipidemia. The aim of the study is to investigate the effect of a complex pharmaceutical composition (CPC) (antioxidants and metabolitotropic agents), which is widely used in medical practice in Ukraine as a multivitamin complex, on experimental metabolic syndrome in rats. The effectof CPC on the correction of experimental MetS in rats, induced by a high content of carbohydrates and fats in the diet, was assessed. MetS in rats was characterized by a decrease in the sensitivity of cells to insulin, increased glucose content, and aviolation of its utilization, prooxidant-antioxidant disbalance. The results of the conducted studies indicate the positive effect of CPC, which contains ethyl esters of omega-3 acids, vitamin E, coenzyme Q10, zinc, vitamin A, biotin, and selenium, on the sensitivity of cells to insulin, glucose utilization, duration of hyperglycemia and indicators of free radical oxidation processes and antioxidant defense systems in rats with experimental MetS. These results prove the feasibility of using CPC to correct metabolic syndrome.
- Klíčová slova
- citlivost buněk na inzulin,
- MeSH
- biotin farmakologie terapeutické užití MeSH
- hyperglykemie farmakoterapie MeSH
- metabolický syndrom * farmakoterapie MeSH
- modely nemocí na zvířatech MeSH
- potkani Wistar MeSH
- potravní doplňky * MeSH
- selen farmakologie terapeutické užití MeSH
- vitamin A farmakologie terapeutické užití MeSH
- vitamin E farmakologie terapeutické užití MeSH
- zinek farmakologie terapeutické užití MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
The growing interest in foods that can be beneficial to human health is bringing into focus some products that have been used locally for centuries but have recently gained worldwide attention. One of these foods is pumpkin seed oil, which has been used in culinary and traditional medicine, but recent data also show its use in the pharmaceutical and cosmetic industries. In addition, some sources refer to it as a potential functional food, mainly because it is obtained from pumpkin seeds, which contain many functional components. However, the production process of the oil may affect the content of these components and consequently the biological activity of the oil. In this review, we have focused on summarizing scientific data that explore the potential of pumpkin seed oil as a functional food ingredient. We provide a comprehensive overview of pumpkin seed oil chemical composition, phytochemical content, biological activity, and safety, as well as the overview of production processes and contemporary use. The main phytochemicals in pumpkin seed oil with health-related properties are polyphenols, phytoestrogens, and fatty acids, but carotenoids, squalene, tocopherols, and minerals may also contribute to health benefits. Most studies have been conducted in vitro and support the claim that pumpkin seed oil has antioxidant and antimicrobial activities. Clinical studies have shown that pumpkin seed oil may be beneficial in the treatment of cardiovascular problems of menopausal women and ailments associated with imbalance of sex hormones.
- MeSH
- antiinfekční látky * MeSH
- antioxidancia farmakologie MeSH
- Cucurbita * chemie MeSH
- funkční potraviny MeSH
- fytoestrogeny MeSH
- fytonutrienty MeSH
- karotenoidy MeSH
- léčivé přípravky MeSH
- lidé MeSH
- mastné kyseliny chemie MeSH
- oleje rostlin chemie farmakologie MeSH
- polyfenoly MeSH
- přísady do jídla * MeSH
- skvalen MeSH
- tokoferoly MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
