fungální enzymy Dotaz Zobrazit nápovědu
- Klíčová slova
- fungální enzymy, degenerativní nemoci,
- MeSH
- akutní nemoc terapie MeSH
- analgetika * terapeutické užití MeSH
- antiflogistika * terapeutické užití MeSH
- antikoagulancia kontraindikace MeSH
- biologická dostupnost MeSH
- biotechnologie MeSH
- chondroitin aplikace a dávkování farmakologie terapeutické užití MeSH
- chronická nemoc farmakoterapie MeSH
- cytokiny antagonisté a inhibitory účinky léků MeSH
- edém * farmakoterapie MeSH
- enzymy MeSH
- fibrinolýza * účinky léků MeSH
- glukosamin * aplikace a dávkování dějiny farmakokinetika farmakologie terapeutické užití MeSH
- ibuprofen aplikace a dávkování farmakologie kontraindikace terapeutické užití MeSH
- imunomodulace * účinky léků MeSH
- inzerce jako téma MeSH
- kombinovaná farmakoterapie MeSH
- lidé MeSH
- osteoartróza diagnóza farmakoterapie MeSH
- paracetamol aplikace a dávkování farmakokinetika terapeutické užití toxicita MeSH
- proteasy * aplikace a dávkování farmakologie kontraindikace terapeutické užití MeSH
- výpočet dávky léku MeSH
- zánět * diagnóza etiologie farmakoterapie prevence a kontrola MeSH
- Check Tag
- lidé MeSH
Activity of some enzymes of a Pb-adapted strain of Rhizopus arrhizus augmented significantly during bioaccumulation of Pb from a chemically defined medium. The mycelium of a Pb-adapted strain exposed to 1 microg/mL Pb in a defined medium for 10 d at 28 +/- 2 degrees C exhibited, relative to a wild-type strain, increased activities of antioxidant enzymes, i.e. SOD, CAT and GPX and of enzymes like AP and PPO involved in defense against pathogens. Another response is a significant increase in the cellular thiol content after 4 d. The responses to Pb exposure thus include an increase in the antioxidant and anti-pathogen defense, and an increased metal-chelating ability.
- MeSH
- antifungální látky metabolismus MeSH
- enzymy metabolismus MeSH
- fungální léková rezistence MeSH
- fungální proteiny metabolismus MeSH
- kultivační média chemie MeSH
- mycelium enzymologie metabolismus účinky léků MeSH
- olovo metabolismus MeSH
- Rhizopus enzymologie metabolismus účinky léků MeSH
- sulfhydrylové sloučeniny metabolismus MeSH
In recent years, there has been an increase in efforts to improve wastewater treatment as the concentration of dangerous pollutants, such as endocrine disrupting chemicals, in wastewater increases. These compounds, which mimic the effect of hormones, have a negative impact on human health and are not easily removed from water. One way to effectively eliminate these pollutants is to use enzymatically activated materials. In this study, we report on the use of laccase from the white rot fungus Trametes versicolor immobilized onto polyamide 6/chitosan (PA6/CHIT) nanofibers modified using two different spacers (bovine serum albumin and hexamethylenediamine). We then tested the ability of the PA6/CHIT-laccase biocatalysts to eliminate a mixture containing 50μM of two endocrine disrupting chemicals: bisphenol A and 17α-ethinylestradiol. The PA6/CHIT nanofiber matrix used in this study not only proved to be a suitable carrier for immobilized and modified laccase but was also efficient in the removal of a mixture of endocrine disrupting chemicals in three treatment cycles.
- MeSH
- biodegradace MeSH
- chitosan chemie MeSH
- endokrinní disruptory metabolismus MeSH
- enzymy imobilizované metabolismus MeSH
- fungální proteiny metabolismus MeSH
- kaprolaktam analogy a deriváty chemie MeSH
- lakasa metabolismus MeSH
- lidé MeSH
- nanovlákna chemie ultrastruktura MeSH
- polymery chemie MeSH
- Trametes enzymologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Under carbon starvation, Aspergillus nidulans released a metallo-proteinase with activities comparable to those of PrtA, the major extracellular serine proteinase of the fungus. The relative molar mass of the enzyme was 19 kDa as determined with both denaturing and renaturing SDS PAGE, while its isoelectric point and pH and temperature optima were 8.6, 5.5 and 65 degrees C, respectively. The enzyme was stable at pH 3.5-10.5 and was still active at 95 degrees C in the presence of azocasein substrate. MALDI-TOF MS analysis demonstrated that the proteinase was encoded by the pepJ gene (locus ID AN7962.3), and showed high similarity to deuterolysin from Aspergillus oryzae. The size of the mature enzyme, its EDTA sensitivity and heat stability also supported the view that A. nidulans PepJ is a deuterolysin-type metallo-proteinase.
Cryptococcus laurentii strain AL(27) demonstrating significant potential for intracellular phytase production was selected by 2-step screening of Antarctic yeasts. The strain showed increased phytase activity in a culture medium with 40 g/L sucrose, KH(2)PO(4) providing 5 mg/L phosphorus, and cultivation temperature of 24 degrees C, which relates it to psychrotrophic microorganisms. The enzyme kinetic characteristics according to sodium phytate were K (m) = 0.98 mmol/L, v (lim) = 33.3 mumol g(-1) min(-1). The enzyme had maximum activity at 40 degrees C and acted within a wide pH range: from 2.0 to 5.5, which is of positive significance for its direct inclusion into the feed of monogastric animals.
Extracellular enzymes produced by Metarhizium anisopliae are believed to play a key role in cuticle hydrolysis. The in-vitro production of cuticle-degrading enzymes, such as chitinase, proteinase, caseinase, lipase and amylase in fourteen isolates of M. anisopliae exhibited significant natural isolate variability. The isolates were also evaluated for chitinase and proteinase enzyme assays in order to quantify the enzyme production. The growth characteristics and colony morphology of the isolates showed variation and few isolates formed sectors and the colonies were either fluffy or powdery. Among the isolates studied, isolate UM2 was found to show good consistence with the results on enzyme measurements as well as the growth characteristics and colony morphology. Such characterization of isolate variability could rationally be used in the selection of isolates for the production of improved myco-pesticides in the integrated pest management programs.
The enzymatic regioselective monopalmitoylation of racemic 9-(2,3-dihydroxypropyl)- adenine (DHPA), an approved antiviral agent, has been performed by an immobilized form of Candida antarctica B lipase (CAL-B) using a 4:1 DMF/hexane mixture as the reaction medium. To improve the chemical yield of the desired monopalmitoylation reaction, solid-phase chemical modifications of the lipase were evaluated. The reaction yield was successfully increased obtaining 100% product after a second treatment of the product solution with fresh immobilised chemically glycosylated-CAL-B.
- MeSH
- adenin analogy a deriváty chemie MeSH
- Candida enzymologie MeSH
- enzymy imobilizované chemie MeSH
- fungální proteiny chemie MeSH
- glykosylace MeSH
- hexany chemie MeSH
- katalýza * MeSH
- lipasa chemie MeSH
- lipoylace MeSH
- polymery chemie MeSH
- rozpouštědla chemie MeSH
- stereoizomerie MeSH
- Publikační typ
- časopisecké články MeSH
Fungal infections constitute a serious clinical problem in the group of patients receiving total parenteral nutrition. The majority of species isolated from infections of the total parenteral nutrition patients belong to Candida genus. The most important factors of Candida spp. virulence are the phenomenon of "phenotypic switching," adhesins, dimorphism of fungal cells and the secretion of hydrolytic enzymes such as proteinases and lipases, including aspartyl proteinases. We determined the proteolytic activity of yeast-like fungal strains cultured from the clinical materials of patients receiving total parenteral nutrition and detected genes encoding aspartyl proteinases in predominant species Candida glabrata--YPS2, YPS4, and YPS6, and Candida albicans--SAP1-3, SAP4, SAP5, and SAP6. C. albicans released proteinases on the various activity levels. All C. glabrata strains obtained from the clinical materials of examined and control groups exhibited secretion of the proteinases. All 13 isolates of C. albicans possessed genes SAP1-3. Gene SAP4 was detected in genome of 11 C. albicans strains, SAP5 in 6, and SAP6 in 11. Twenty-six among 31 of C. glabrata isolates contained YPS2 gene, 21 the YPS4 gene, and 28 the YPS6 gene. We observed that clinical isolates of C. albicans and C. glabrata differed in SAPs and YPSs gene profiles, respectively, and displayed differentiated proteolytic activity. We suppose that different sets of aspartyl proteinases genes as well as various proteinase-activity levels would have the influence on strains virulence.
- MeSH
- Candida albicans enzymologie genetika izolace a purifikace MeSH
- Candida glabrata enzymologie genetika izolace a purifikace MeSH
- faktory virulence genetika metabolismus MeSH
- genom fungální MeSH
- geny hub MeSH
- kandidóza mikrobiologie MeSH
- lidé MeSH
- parenterální výživa úplná škodlivé účinky MeSH
- proteasy genetika sekrece MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
A simple procedure for the synthesis of magnetic fluid (ferrofluid) stabilized by poly(methacrylic acid) has been developed. This ferrofluid was used to prepare a novel type of magnetically responsive chitosan-based composite material. Both ferrofluid and magnetic chitosan composite were characterized by a combination of microscopy (optical microscopy, TEM, SEM), scattering (static and dynamic light scattering, SANS) and spectroscopy (FTIR) techniques. Magnetic chitosan was found to be a perspective material for various bioapplications, especially as a magnetic carrier for immobilization of enzymes and cells. Lipase from Candida rugosa was covalently attached after cross-linking and activation of chitosan using glutaraldehyde. Baker's yeast cells (Saccharomyces cerevisiae) were incorporated into the chitosan composite during its preparation; both biocatalysts were active after reaction with appropriate substrates.
- MeSH
- Candida enzymologie MeSH
- chitosan chemie MeSH
- enzymy imobilizované chemie MeSH
- fungální proteiny chemie MeSH
- imobilizované buňky cytologie metabolismus MeSH
- kyseliny polymethakrylové chemie MeSH
- lipasa chemie MeSH
- magnetismus * MeSH
- Saccharomyces cerevisiae cytologie metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
