Human immunodeficiency virus type 1 (HIV-1) entry is mediated by the interaction between a variably glycosylated envelope glycoprotein (gp120) and host-cell receptors. Approximately half of the molecular mass of gp120 is contributed by N-glycans, which serve as potential epitopes and may shield gp120 from immune recognition. The role of gp120 glycans in the host immune response to HIV-1 has not been comprehensively studied at the molecular level. We developed a new approach to characterize cell-specific gp120 glycosylation, the regulation of glycosylation, and the effect of variable glycosylation on antibody reactivity. A model oligomeric gp120 was expressed in different cell types, including cell lines that represent host-infected cells or cells used to produce gp120 for vaccination purposes. N-Glycosylation of gp120 varied, depending on the cell type used for its expression and the metabolic manipulation during expression. The resultant glycosylation included changes in the ratio of high-mannose to complex N-glycans, terminal decoration, and branching. Differential glycosylation of gp120 affected envelope recognition by polyclonal antibodies from the sera of HIV-1-infected subjects. These results indicate that gp120 glycans contribute to antibody reactivity and should be considered in HIV-1 vaccine design.

• MeSH
• AIDS imunologie   MeSH
• buněčné linie MeSH
• buňky Hep G2 metabolismus   MeSH
• ELISA metody   MeSH
• glykosidhydrolasy metabolismus   MeSH
• glykosylace MeSH
• HIV obalový protein gp120 genetika   imunologie   izolace a purifikace   metabolismus   MeSH
• HIV séropozitivita imunologie   metabolismus   MeSH
• HIV-1 imunologie   metabolismus   MeSH
• hmotnostní spektrometrie MeSH
• Jurkat buňky metabolismus   MeSH
• komplementární DNA genetika   MeSH
• lektin vázající mannosu genetika   MeSH
• lidé MeSH
• mannosa metabolismus   MeSH
• nádorové buněčné linie MeSH
• oligosacharidy chemie   izolace a purifikace   MeSH
• plazmidy MeSH
• polysacharidy chemie   izolace a purifikace   MeSH
• protilátky imunologie   MeSH
• specificita protilátek MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, N.I.H., Extramural MeSH
• Názvy látek
• glykosidhydrolasy MeSH
• gp120 protein, Human immunodeficiency virus 1 MeSH Prohlížeč
• HIV obalový protein gp120 MeSH
• komplementární DNA MeSH
• lektin vázající mannosu MeSH
• mannosa MeSH
• oligosacharidy MeSH
• polysacharidy MeSH
• protilátky MeSH