Covalent labeling in combination with mass spectrometry is a powerful approach used in structural biology to study protein structures, interactions, and dynamics. Recently, the toolbox of covalent labeling techniques has been expanded with fast fluoroalkylation of proteins (FFAP). FFAP is a novel radical labeling method that utilizes fluoroalkyl radicals generated from hypervalent Togni reagents for targeting aromatic residues. This report further demonstrates the benefits of FFAP as a new method for structural characterization of therapeutic antibodies and interaction interfaces of antigen-antibody complexes. The results obtained from human trastuzumab and its complex with human epidermal growth factor receptor 2 (HER2) correlate well with previously published structural data and demonstrate the potential of FFAP in structural biology.

• MeSH
• alkylace MeSH
• footprinting proteinů metody   MeSH
• halogenace MeSH
• imunokomplex chemie   MeSH
• lidé MeSH
• mapování epitopu * metody   MeSH
• monoklonální protilátky chemie   imunologie   MeSH
• receptor erbB-2 * chemie   imunologie   MeSH
• trastuzumab * chemie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

We report for the first time the use of two live-cell imaging agents from the group of luminescent transition metal complexes (IRAZOLVE-MITO and REZOLVE-ER) as cathodoluminescent probes. This first experimental demonstration shows the application of both probes for the identification of cellular structures at the nanoscale and near the native state directly in the cryo-scanning electron microscope. This approach can potentially be applied to correlative and multimodal approaches and used to target specific regions within vitrified samples at low electron beam energies.

• MeSH
• iridium chemie   MeSH
• komplexní sloučeniny * chemie   MeSH
• luminiscence MeSH
• rhenium * chemie   MeSH
• teplota MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• iridium MeSH
• komplexní sloučeniny * MeSH
• rhenium * MeSH

Adenylate cyclase toxin (CyaA) is released in the course of B. pertussis infection in the host's respiratory tract in order to suppress its early innate and subsequent adaptive immune defense. CD11b-expressing dendritic cells (DC), macrophages and neutrophils are professional phagocytes and key players of the innate immune system that provide a first line of defense against invading pathogens. Recent findings revealed the capacity of B. pertussis CyaA to intoxicate DC with high concentrations of 3',5'-cyclic adenosine monophosphate (cAMP), which ultimately skews the host immune response towards the expansion of Th17 cells and regulatory T cells. CyaA-induced cAMP signaling swiftly incapacitates opsonophagocytosis, oxidative burst and NO-mediated killing of bacteria by neutrophils and macrophages. The subversion of host immune responses by CyaA after delivery into DC, macrophages and neutrophils is the subject of this review.

• Klíčová slova
• T-helper cells, immune response, intracellular pathways, phagocytosis,
• MeSH
• adenylátcyklasový toxin imunologie   MeSH
• AMP cyklický chemie   MeSH
• Bordetella pertussis MeSH
• buněčná imunita MeSH
• dendritické buňky imunologie   MeSH
• dýchací soustava imunologie   mikrobiologie   MeSH
• fagocytóza MeSH
• interakce hostitele a patogenu MeSH
• lidé MeSH
• makrofágy imunologie   MeSH
• neutrofily imunologie   MeSH
• pertuse imunologie   MeSH
• regulační T-lymfocyty imunologie   MeSH
• signální transdukce MeSH
• slizniční imunita MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• adenylátcyklasový toxin MeSH
• AMP cyklický MeSH