Background: Zinc oxide nanoparticles are safe, non-toxic, and biocompatible. These NPs are used in food packaging materials, self-cleaning glass, ceramics, deodorants, sunscreens, paints, coatings, ointments, lotions, and as preservatives. This study explored the biological potential of ZnO nanoparticles synthesized using H. rariflorum. Methods: In vitro antibacterial and antifungal activities against Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Salmonella typhi, Candida albicans, Penicillium notatum, Aspergillus flavus, Aspergillus niger and Aspergillus solani were determined. Antioxidant activity was explored using the DPPH radical scavenging method. In vivo analgesic, antipyretic and sedative potential of synthesized nanoparticles was investigated using a mouse model. Results: SEM with various magnification powers showed that some particles were spherical while some were aggregated, flake-shaped, and hexagonal with rough and irregular surfaces. The EDX analysis revealed Zn (12.63%), O (22.83%) and C (63.11%) with trace quantities of Si (0.40%), Ca (0.54%) and P (0.49%). The XRD pattern indicated an amorphous state, with no peaks observed throughout the spectrum. The UV-visible spectrophotometry revealed a characteristic absorption peak at 375 nm, indicating the presence of ZnO nanoparticles. Fourier Transform Infrared Spectroscopy (FTIR) displayed several small peaks between 1793 and 2370 cm-1, providing evidence of the presence of different kinds of organic compounds with different functional groups. ZnO-NPs showed dose-dependent antibacterial and antifungal potential against all strains. Staphylococcus aureus and Candida albicans were the most susceptible strains. The nanoparticles exhibited a maximum antioxidant effect of 85.28% at 100 μg/mL. In this study, the acute toxicity test showed no mortality, and normal behavior was observed in mice at ZnO-NP doses of 5, 10, and 20 mg/kg. For analgesic and antipyretic activities, a two-way ANOVA revealed that dose, time, and the interaction between dose and time were significant. In contrast, the samples had a non-significant effect on sedative activity. Conclusions: This innovative study suggests a potential use of plant resources for managing microbes and treating various diseases, providing a scientific basis for the traditional use of H. rariflorum.
- Klíčová slova
- acute toxicity, analgesic, antibacterial, antifungal, antioxidant, antipyretic, sedative,
- Publikační typ
- časopisecké články MeSH
Leaf area index (LAI) is an essential canopy variable describing the amount of foliage in an ecosystem. The parameter serves as the interface between green components of plants and the atmosphere, and many physiological processes occur there, primarily photosynthetic uptake, respiration, and transpiration. LAI is also an input parameter for many models involving carbon, water, and the energy cycle. Moreover, ground-based in situ measurements serve as the calibration method for LAI obtained from remote sensing products. Therefore, straightforward indirect optical methods are necessary for making precise and rapid LAI estimates. The methodological approach, advantages, controversies, and future perspectives of the newly developed LP 110 optical device based on the relation between radiation transmitted through the vegetation canopy and canopy gaps were discussed in the protocol. Furthermore, the instrument was compared to the world standard LAI-2200 Plant Canopy Analyzer. The LP 110 enables more rapid and more straightforward processing of data acquired in the field, and it is more affordable than the Plant Canopy Analyzer. The new instrument is characterized by its ease of use for both above- and below-canopy readings due to its greater sensor sensitivity, in-built digital inclinometer, and automatic logging of readings at the correct position. Therefore, the hand-held LP 110 device is a suitable gadget for performing LAI estimation in forestry, ecology, horticulture, and agriculture based on the representative results. Moreover, the same device also enables the user to take accurate measurements of incident photosynthetically active radiation (PAR) intensity.
Plant growth is affected by light availability, light capture, and the efficiency of light energy utilisation within the photosynthetic uptake processes. The radiation use efficiency (RUE) of four even-aged, fully stocked mature Norway spruce stands along a temperature, precipitation, and altitudinal gradient of the Czech Republic was investigated. A new straightforward, methodological approach involving an analysis of digital hemispherical photographs for RUE estimation was applied. The highest annual RUE value (0.72 g MJ-1) was observed in the stand characterised by the lowest mean annual air temperature, the highest annual amount of precipitation, located at the highest altitude, and with the lowest site index reflecting site fertility. From the viewpoint of global climate change mitigation, this stand fixed 4.14 Mg ha-1 and 13.93 Mg ha-1 of carbon units and CO2 molecules into above-ground biomass, respectively. The lowest RUE value (0.21 g MJ-1) within the studied growing season was found in the stand located at the lowest altitude representing the site with the highest mean air temperature and the lowest amount of precipitation where 1.27 Mg ha-1 and 4.28 Mg ha-1 of carbon units and CO2 molecules, respectively, were fixed. From the tested meteorological variables (mean air temperature, the monthly sums of temperature, precipitation, and air humidity), RUE was only significantly dependent on air temperature. Therefore, global warming can lead to diminishing RUE and carbon sequestration in Norway spruce stands, especially at low altitudes.
- Klíčová slova
- Above-ground biomass, Carbon sequestration, Hemispherical photography, Picea abies, Solar radiation,
- MeSH
- klimatické změny * MeSH
- smrk * MeSH
- stromy MeSH
- teplota MeSH
- transpirace rostlin MeSH
- uhlík MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika MeSH
- Norsko MeSH
- Názvy látek
- uhlík MeSH
Accurate estimations of leaf area index (LAI), defined as half of the total leaf surface area per unit of horizontal ground surface area, are crucial for describing the vegetation structure in the fields of ecology, forestry, and agriculture. Therefore, procedures of three commercially used methods (litter traps, needle technique, and a plant canopy analyzer) for performing LAI estimation were presented step-by-step. Specific methodological approaches were compared, and their current advantages, controversies, challenges, and future perspectives were discussed in this protocol. Litter traps are usually deemed as the reference level. Both the needle technique and the plant canopy analyzer (e.g., LAI-2000) frequently underestimate LAI values in comparison with the reference. The needle technique is easy to use in deciduous stands where the litter completely decomposes each year (e.g., oak and beech stands). However, calibration based on litter traps or direct destructive methods is necessary. The plant canopy analyzer is a commonly used device for performing LAI estimation in ecology, forestry, and agriculture, but is subject to potential error due to foliage clumping and the contribution of woody elements in the field of view (FOV) of the sensor. Eliminating these potential error sources was discussed. The plant canopy analyzer is a very suitable device for performing LAI estimations at the high spatial level, observing a seasonal LAI dynamic, and for long-term monitoring of LAI.
BACKGROUND: In Czechia, only about a quarter of people suffering from the Alzheimer's disease (AD) receive (usually belated) treatment. Because of their more rapid cognitive decline, untreated patients require extensive assistance with basic daily activities earlier than those receiving treatment. This assistance provided at home and nursing homes represents a substantial economic burden. AIMS OF THE STUDY: To calculate lifetime costs of care per AD patient and to evaluate potential care savings from early treatment. METHODS: We use Monte Carlo simulation to model lifetime societal costs of care per patient under two different scenarios. In the first one, a cohort of 100,000 homogeneous patients receives usual care under which the majority of patients are undiagnosed or diagnosed late. The second scenario models a hypothetical situation in which an identical cohort of patients starts receiving treatment early after the disease onset. Data on the rates of cognitive decline for treated and untreated patients, and survival probability for AD patients are derived from foreign clinical studies. Information on costs and population characteristics is compiled on the basis of published Czech research and databases. RESULTS: Early treatment of AD decreases social lifetime costs of care. This result holds true regardless of gender, age at which the disease is contracted, or whether the patient lives at home or uses a social residential service. The potential savings amount up to Euro 26,800 (23,500) per woman (man), being negatively correlated with the age at which the disease onsets as well as the delay between the onset and treatment initiation DISCUSSION: The results suggest that early treatment of AD would decrease costs of care in Czechia. The main limitation of the simulation arises from the fact that missing domestic information was substituted by input from foreign clinical trials or simplifying assumptions. Because of insufficient data, we do not model hospitalization risk; on the other hand, introduction of this risk into our model would likely increase the savings from early treatment. IMPLICATIONS FOR HEALTH POLICIES: Makers of AD policies ought to appreciate the trade-off between costs of daily assistance in untreated patients and health care costs in treated patients, notwithstanding that the costs of assistance are largely born by households rather than public budgets. Our results show that the savings on costs of assistance brought about by early treatment would exceed the additional costs of treatment. IMPLICATIONS FOR FURTHER RESEARCH: A number of missing or insufficient data about the Czech Alzheimer's population were identified. In addition, to determine the total societal cost-effect of early treatment, further research ought to evaluate the related increase in detection costs. Finally, it should also assess cost-effectiveness of early treatment by considering its impact on patients' utility.
- MeSH
- Alzheimerova nemoc diagnóza ekonomika terapie MeSH
- časná diagnóza * MeSH
- časná lékařská intervence ekonomika MeSH
- ekonomické modely * MeSH
- lidé středního věku MeSH
- lidé MeSH
- metoda Monte Carlo MeSH
- náklady na zdravotní péči statistika a číselné údaje MeSH
- osobní újma zaviněná nemocí MeSH
- pečovatelské domovy ekonomika MeSH
- programy národního zdraví ekonomika MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- služby domácí péče ekonomika MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Československo MeSH
AIM: To provide an overview of gambling and associated problems in the Czech Republic, including an overview of the historical context, legislation, prevalence, treatment and research base and agenda. METHODS: A review of literature and relevant sources. RESULTS: The trajectory of gambling patterns in the territory of the Czech Republic in the 20th century reflected broad socio-political changes. Those included significant expansion between the wars, strict state control and bans on some gambling activities during the communist regime and finally dynamic development characterized by a boom in electronic gaming machines (EGMs) and increasing accessibility of gambling facilities after 1989, which aggravated gambling-related problems. Many municipalities have banned EGMs, which has created conflict in regulation at state and municipal levels. The draft gambling law prepared in 2014 aims to clarify the regulatory framework. Before 2012 there was only sporadic research interest in gambling, but in 2012 the first complex research on gaming and problem gambling in the Czech population took place. The estimated prevalence of problem gambling is currently 2% in the population aged 15-64 years. Preventive measures, counselling and treatment services for problem gamblers are limited. CONCLUSIONS: Weak and ineffective regulation of the gambling market in the Czech Republic during the past 20 years, despite the large growth in gambling, has led to inadequate prevention and response to problem gambling which has become a considerable public health, social and political issue.
- Klíčová slova
- Czech Republic, gambling, prevalence, problem gambling, regulation, research, treatment,
- MeSH
- behaviorální výzkum * dějiny trendy MeSH
- dějiny 18. století MeSH
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- dějiny starověku MeSH
- dějiny středověku MeSH
- hráčství * epidemiologie dějiny terapie MeSH
- lidé MeSH
- návykové chování * epidemiologie dějiny terapie MeSH
- prevalence MeSH
- Check Tag
- dějiny 18. století MeSH
- dějiny 20. století MeSH
- dějiny 21. století MeSH
- dějiny starověku MeSH
- dějiny středověku MeSH
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- historické články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
