Effects of low-frequency electromagnetic fields (LF EMF) on the activation of different tissue recovery processes have already been fully understood. Preliminary recent data demonstrated that a special case of sinusoidal electromagnetic fields, known as amplitude-modulated currents (AMC) could have a potential to accelerate the cell metabolism or cell migration. An AMC generator was designed to generate sinusoidal induced electric currents with the amplitude modulation and the harmonic carrier frequency of 5,000 Hz was modulated by frequencies of 1 to 100 Hz. The magnetic field peak was 6 mT, electric field intensity 2 V/m and the current density of induced electrical currents was approximately 1 A/m(2). The coil of the generator was adapted to easy handling and safe integration into the shelf of the CO(2) incubator. The shelf with the coil was prepared for the introduction of cells in standard plastic in vitro chambers. The tests focused on cells with migratory capacity after injury or during immunological processes and thus, mesenchymal stromal cells (MSC), dendritic cells (DC), and fibroblasts were chosen. The tests involved exposures of the cells to LF EMF (180 min/day) every day, for a period of three days, before examining them for cell death, morphology changes, and CD markers. The samples were tested by using MTT assay and the effects on the intracellular concentration of reactive oxygen species were quantified. The cell migration was finally measured with the help of the transwell migration assay. None of the cell types showed any decrease in the cell viability after the LF EMF application and the cells displayed minimum changes in reactive oxygen species. Functional changes (acceleration of cell migration) after AMC exposure were statistically significant for the MSC samples only. The acceleration of MSCs is associated with the production of MMP by these cells. The EMF has a potential to be a safe, clinically applicable selective activator of MSC homing, MSC paracrine production, and subsequent regeneration processes.

• MeSH
• buňky 3T3 MeSH
• dendritické buňky fyziologie   MeSH
• elektromagnetická pole * MeSH
• fibroblasty fyziologie   MeSH
• lidé MeSH
• matrixová metaloproteinasa 2 metabolismus   MeSH
• mezenchymální kmenové buňky fyziologie   MeSH
• myši MeSH
• parakrinní signalizace MeSH
• pohyb buněk * MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• myši MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• matrixová metaloproteinasa 2 MeSH

Lymphocytes from former uranium miners who finished work underground one or more decades ago were analysed with respect to possibly persisting genetic damage induced by their radiation exposure. A modified micronucleus-centromere test was used which determined the frequency of micronucleus-containing binucleate cells after cytochalasin B treatment and the percentage of centromere-free micronuclei, assessed with the help of immunofluorescence labeling of centromere protein B. Whereas the overall frequency of micronucleus-containing cells was not significantly elevated above the level found in a control group, former miners showed a greater percentage of centromere-free micronuclei, i.e. micronuclei containing only acentric fragments. Our results are in excellent agreement with those of an earlier uranium miner study and lend support to the assumption that genetic damage from alpha radiation can persist for many years after exposure, possibly due to genomic instability. The frequency of micronucleus-containing cells, but not the percentage of centromere-free micronuclei, significantly increased with time since last exposure in the mines. This can be attributed, at least in part, to the fact that miners who have finished working underground longer ago tend to be older, and there is an increase of the frequency of micronucleus-containing cells with age.

• MeSH
• alfa částice škodlivé účinky   MeSH
• časové faktory MeSH
• dospělí MeSH
• hornictví * MeSH
• lidé středního věku MeSH
• lidé MeSH
• lymfocyty účinky záření   MeSH
• mikrojaderné testy MeSH
• mikrojádra chromozomálně defektní účinky záření   MeSH
• mladý dospělý MeSH
• nestabilita genomu účinky záření   MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• uran škodlivé účinky   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladý dospělý MeSH
• mužské pohlaví MeSH
• senioři nad 80 let MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• uran MeSH

The paper explores the quantities and units used in radiation protection with special emphasis on their applications in occupational exposure control. An overview of the current situation reveals that there seem to be too many different quantities associated with the same unit. Some of these quantities are defined in a quite complicated manner and, therefore, may cause some confusion in their interpretation and practical use in the field. Some suggestions towards the simplification of the present system are also proposed.

• MeSH
• dávka záření MeSH
• interpretace statistických dat MeSH
• ionizující záření MeSH
• lidé MeSH
• monitorování radiace metody   normy   MeSH
• pracovní expozice prevence a kontrola   MeSH
• radiační ochrana metody   normy   MeSH
• radiometrie metody   normy   MeSH
• rozvoj plánování MeSH
• stochastické procesy MeSH
• systémová analýza MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH

The paper discusses the situation in the Czech Republic regarding past and present uranium mining activities with emphasis on the evaluation of the exposure of underground miners in the Rožná Uranium Mine, which is currently the only active mine in the country and practically in the entire European Union. The total effective dose has been summarised taking into account all three major components, namely radon short-lived decay products, long-lived alpha emitters in ore dust and penetrating external gamma radiation. The average and maximum values of the effective dose as well as the collective effective dose of underground miners are also presented. The purpose of the paper is to document the miners' exposures during a period of 6 years in a uranium mine where conditions including the ore grade and methods of mining showed recently some changes that may affect the individual components of the total effective dose.

• MeSH
• dávka záření MeSH
• dceřiné produkty radonu MeSH
• hornictví * MeSH
• lidé MeSH
• monitorování radiace přístrojové vybavení   metody   MeSH
• pracovní expozice prevence a kontrola   MeSH
• radiační ochrana přístrojové vybavení   metody   MeSH
• radioaktivní látky znečišťující vzduch MeSH
• radiometrie přístrojové vybavení   metody   MeSH
• radon MeSH
• termoluminiscenční dozimetrie přístrojové vybavení   MeSH
• uran analýza   MeSH
• záření gama MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• dceřiné produkty radonu MeSH
• radioaktivní látky znečišťující vzduch MeSH
• radon MeSH
• uran MeSH

OBJECTIVE: Low-level laser therapy (LLLT) treatment for pain caused by temporomandibular joint disorders (TMD) was investigated in a controlled study comparing applied energy density, subgroups of TMD, and duration of disorders. BACKGROUND DATA: Although LLLT is a physical therapy used in the treatment of musculoskeletal disorders, there is little evidence for its effectiveness in the treatment of TMD. METHODS: The study group of 61 patients was treated with 10 J/cm(2) or 15 J/cm(2), and the control group of 19 patients was treated with 0.1 J/cm(2). LLLT was performed by a GaAlAs diode laser with output of 400 mW emitting radiation wavelength of 830 nm in 10 sessions. The probe with aperture 0.2 cm(2) was placed over the painful muscle spots in the patients with myofascial pain. In patients with TMD arthralgia the probe was placed behind, in front of, and above the mandibular condyle, and into the meatus acusticus externus. Changes in pain were evaluated by self-administered questionnaire. RESULTS: Application of 10 J/cm(2) or 15 J/cm(2) was significantly more effective in reducing pain compared to placebo, but there were no significant differences between the energy densities used in the study group and between patients with myofascial pain and temporomandibular joint arthralgia. Results were marked in those with chronic pain. CONCLUSION: The results suggest that LLLT (application of 10 J/cm(2) and 15 J/cm(2)) can be considered as a useful method for the treatment of TMD-related pain, especially long lasting pain.

• MeSH
• dospělí MeSH
• laserová terapie s nízkou intenzitou světla metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• měření bolesti MeSH
• mladiství MeSH
• nemoci temporomandibulárního kloubu patofyziologie   radioterapie   MeSH
• obličejová bolest patofyziologie   radioterapie   MeSH
• placeba MeSH
• průzkumy a dotazníky MeSH
• rozdělení chí kvadrát MeSH
• senioři MeSH
• výsledek terapie MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mladiství MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• klinické zkoušky kontrolované MeSH
• práce podpořená grantem MeSH
• Názvy látek
• placeba MeSH

OBJECTIVE: This case report describes the treatment of a patient with arthralgia of the temporomandibular joint (TMJ) caused by disc displacement. BACKGROUND DATA: The goal of the treatment of TMJ arthralgia is to decrease pain by promotion of the musculoskeletal system's natural healing ability. METHODS: This report describes the complex treatment of TMJ arthralgia. Low-level laser therapy (LLLT) was chosen for its antiinflammatory and analgesic effects. Laser therapy was carried out using the GaAlAs diode laser with an output power of 400 mW, emitting radiation with a wavelength of 830 nm, and having energy density of 15 J/cm2; the laser radiation was applied by contact mode on four targeted spots in 10 sessions. Physiotherapy was recommended to this patient to prevent the injury of intraarticular tissue caused by incorrect movement during opening of the mouth. Splint stabilization and prosthetic treatment were used to reduce overloading of the TMJ, resulting from unstable occlusion and to help repositioning of the dislocated disc. RESULTS: Five applications of LLLT led to decrease of pain in the area of the TMJ on the Visual Analog Scale, from 20 to 5 mm. The anti-inflammatory effect of the laser was confirmed by thermographic examination. Before treatment, the temperature differences between the areas of the normal TMJ and TMJ with arthralgia was higher than 0.5 degrees C. However, at the conclusion of LLLT, temperatures in the areas surrounding the TMJ were equalized. CONCLUSION: This study showed the effectiveness of complex non-invasive treatment in patients with arthralgia of the TMJ. The analgesic and anti-inflammatory effects of LLLT were confirmed by infrared thermography.

• MeSH
• artralgie radioterapie   MeSH
• laserová terapie s nízkou intenzitou světla * MeSH
• lidé středního věku MeSH
• lidé MeSH
• temporomandibulární kloub * MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH
• práce podpořená grantem MeSH

PURPOSE: We studied the relationship between type II pneumocytes number and alveolar septal thickness during different time after sublethal whole-thorax irradiation of rats and we investigated the influence of pentoxifylline (TNF-alpha inhibitor). MATERIALS AND METHODS: Wistar rats were exposed to 15 Gy thoracic irradiation and pentoxifylline (35 mg/kg) twice a week. Lungs were examined histologically and immunohistochemically at intervals ranging from 1-12 weeks and alveolar septal thickness, number of type II pneumocytes (identified by immunoreactivity for cytokeratin 18), and neutrophile granulocytes were counted. RESULTS: Significant increase of alveolar septal thickness and type II pneumocytes depletion 3 weeks after irradiation were found. Correlation of these markers was r = -0.759. Pentoxifylline significantly inhibits increased alveolar septal thickness without the influence on type II pneumocytes number. Neutrophil penetration started 5 weeks after irradiation in non-treated animals, 8 weeks after irradiation in PTX-treated rats. CONCLUSIONS: We suggest that pneumocytes depletion is linked to increased vascular permeability, and pentoxifylline therapy does not influence on pneumocytes kinetics after irradiation.

• MeSH
• imunohistochemie MeSH
• keratiny analýza   MeSH
• krysa rodu Rattus MeSH
• pentoxifylin farmakologie   MeSH
• plíce chemie   účinky léků   patologie   účinky záření   MeSH
• plicní alveoly účinky léků   patologie   účinky záření   MeSH
• potkani Wistar MeSH
• radiační pneumonitida patologie   MeSH
• TNF-alfa antagonisté a inhibitory   MeSH
• vazodilatancia farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• keratiny MeSH
• pentoxifylin MeSH
• TNF-alfa MeSH
• vazodilatancia MeSH

We measured number of bcl-2, apoptotic, neutrophil, and surfactant apoprotein D (SP-D) positive cells in irradiated rat lungs during different time points after the sublethal whole-thorax irradiation of rats. We also investigated the influence of pentoxifylline (PTX) therapy on these markers. Wistar rats were given 15 Gy thoracic irradiation and PTX (35 mg/kg) twice a week. Animals were examined histologically and imunohistochemically at intervals from 1-12 weeks. In non-treated rats compared with treated rats, bcl-2 expression was significantly inhibited from 4 weeks after irradiation. A higher apoptosis presence in non-treated rats from 4 weeks was found and apoptosis development in PTX-treated animals was delayed and started 8 weeks after irradiation. Similar differences were measured during neutrophil granulocytes examination. Neutrophil penetration in non-treated rats was found 5 weeks after irradiation in contrast to the RP onset of PTX-treated animals 8 weeks after irradiation. The number of SP-D positive cells in non-treated rats observed until 5 weeks after irradiation was higher than in the control group. PTX-treated animals expressed higher number of SP-D positive cells during the whole experiment than the control group. We suggest that apoptosis is linked to neutrophil granulocyte actions during the RP onset and that PTX-therapy causes diminished inflammation development.

• MeSH
• apoptóza účinky záření   MeSH
• glykoproteiny metabolismus   MeSH
• imunohistochemie MeSH
• krysa rodu Rattus MeSH
• neutrofily patologie   MeSH
• pentoxifylin farmakologie   MeSH
• plíce metabolismus   patologie   účinky záření   MeSH
• plicní surfaktanty metabolismus   MeSH
• potkani Wistar MeSH
• protein D asociovaný s plicním surfaktantem MeSH
• protoonkogenní proteiny c-bcl-2 metabolismus   MeSH
• radiační pneumonitida farmakoterapie   metabolismus   patologie   MeSH
• radioprotektivní látky farmakologie   MeSH
• zvířata MeSH
• Check Tag
• krysa rodu Rattus MeSH
• mužské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• glykoproteiny MeSH
• pentoxifylin MeSH
• plicní surfaktanty MeSH
• protein D asociovaný s plicním surfaktantem MeSH
• protoonkogenní proteiny c-bcl-2 MeSH
• radioprotektivní látky MeSH

The present communication brings a contribution to the confrontation of two treatment methods in enthesopathies (painful overload syndromes). In group A, 26 patients were treated by a combination of systemic enzyme therapy (SET) and non-invasive laser therapy. In group B, 13 persons were treated only by therapeutic laser. The average age in group A and B was of 39.3 and 41.8 years, respectively. The representation of men and women in the two groups was almost identical. The intensity of the pain prior to the treatment and after the treatment, specified by each patient with the help of a ten-grade scale, was evaluated by the variance analysis. The combined treatment by the SET and therapeutic laser was statistically significantly more efficient (p = 0.02). On the other hand, there was also a significant difference between the two groups in baseline values of average pain intensities. These values were of 7.0 and 5.2 in groups A and B, respectively. The average value of the pain intensity in all the 39 patients was of 6.6 prior to the treatment and it was reduced to 3.5 after the treatment.

• MeSH
• aplikace orální MeSH
• bromelainy aplikace a dávkování   MeSH
• dospělí MeSH
• enzymy aplikace a dávkování   MeSH
• fixní kombinace léků MeSH
• imunologické faktory aplikace a dávkování   MeSH
• kombinovaná terapie MeSH
• laserová terapie * MeSH
• lidé MeSH
• měření bolesti MeSH
• revmatické nemoci terapie   MeSH
• rutin aplikace a dávkování   analogy a deriváty   MeSH
• trypsin aplikace a dávkování   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bromelainy MeSH
• enzymy MeSH
• fixní kombinace léků MeSH
• imunologické faktory MeSH
• rutin sulfate MeSH Prohlížeč
• rutin MeSH
• trypsin MeSH

This paper reports on the antitumor activity of BS RNase on human melanoma and mouse seminoma. Human melanoma cells established in culture were extremely susceptible to BS RNase, administered in concentrations ranging from 1-100 microg/ml. Concentrations of BS RNase over 10 microg/ml caused complete inhibition of cell growth. Bovine pancreatic ribonuclease (RNase A), a prototype of the ribonuclease superfamily, did not exert any effect under these conditions. Based on our previous results, athymic mice bearing human melanoma or mouse seminoma were treated with intratumoral administration of BS RNase (12.5 mg/kg b.w.). This dose was injected for five consecutive days excluding weekends. The intratumoral administration of BS RNase to nude mice bearing human melanoma showed a significant antitumor effect. There were no tumors seen in eighty percent of mice treated for three weeks, and tumors in the other mice diminished significantly. After some delay the tumors started to regrow. Prolonging of the treatment to five weeks had a similar effect. The effect of BS RNase on mouse seminoma was well pronounced. Five to seven doses of BS RNase were sufficient to eliminate tumors in all treated mice. However, as in the previous experiment, the growth of tumor tissue later reappeared.

• MeSH
• antitumorózní látky terapeutické užití   MeSH
• endoribonukleasy terapeutické užití   MeSH
• léky antitumorózní - screeningové testy MeSH
• lidé MeSH
• melanom farmakoterapie   MeSH
• myši nahé MeSH
• myši MeSH
• nádorové buňky kultivované MeSH
• pankreatická ribonukleasa terapeutické užití   MeSH
• seminom farmakoterapie   MeSH
• testikulární nádory farmakoterapie   MeSH
• viabilita buněk účinky léků   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antitumorózní látky MeSH
• endoribonukleasy MeSH
• pankreatická ribonukleasa MeSH
• ribonuclease SPL MeSH Prohlížeč