The objective of this study was to investigate the effects of a preparation from herbal extracts (PHE) on libido and semen quality in breeding artificial insemination boars. Ten fertile boars were divided into control and experimental groups according to significant difference of libido. There were no differences in semen quality between groups. Animals were fed a commercial feeding mixture for boars. The feeding mixture for the experimental group was enriched with PHE, which was prepared from Eurycoma longifolia, Tribulus terrestris and Leuzea carthamoides. Duration of the experiment was 10 weeks. Samples of ejaculate were collected weekly. Libido was evaluated according to a scale of 0-5 points. Semen volume, sperm motility, percentage of viable spermatozoa, sperm concentration, morphologically abnormal spermatozoa, daily sperm production and sperm survival were assessed. Amounts of mineral components and free amino acids were analysed in seminal plasma. Significant differences were found in these parameters: libido (4.05 ± 0.22 vs 3.48 ± 0.78; p < 0.001), semen volume (331.75 ± 61.91 vs 263.13 ± 87.17 g; p < 0.001), sperm concentration (386.25 ± 107.95 vs 487.25 ± 165.50 × 10(3) /mm(3); p < 0.01), morphologically abnormal spermatozoa (15.94 ± 11.08 vs 20.88 ± 9.19%; p < 0.001) and Mg concentration (28.36 ± 11.59 vs 20.27 ± 13.93 mm; p < 0.05). The experimental group's libido was increased by 20% in comparison with the beginning of the experiment. Results of this study showed positive effect of PHE on libido and some parameters of boar semen quality.
- MeSH
- analýza spermatu veterinární MeSH
- krmivo pro zvířata MeSH
- prasata fyziologie MeSH
- rostlinné přípravky farmakologie MeSH
- rozvrh dávkování léků MeSH
- sexuální chování zvířat účinky léků MeSH
- sperma chemie MeSH
- spermie účinky léků fyziologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- klinické zkoušky kontrolované MeSH
- práce podpořená grantem MeSH
- Názvy látek
- rostlinné přípravky MeSH
ABSTRACT Coumarins, naturally occurring compounds derived from benzopyran, have recently been studied extensively for their antioxidant properties. A lot of coumarins have been isolated and identified from natural sources and many others have been synthesized. It is also known that pharmacological and biochemical properties and thus also therapeutic application of simple coumarins depend upon the pattern of their substitution. As a part of studies of biological effects, four naturally occurring coumarins and 18 synthesized analogs of several compounds were assayed for 2,2-diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. For this purpose the highly reliable DPPH test modified to be performed by sequential injection analysis (SIA) system was used. This in our laboratory-developed method was originally proposed for antioxidant screening of large series of plant extracts. In this assay, the DPPH test using the SIA method was used for fast and sensitive evaluation of EC(50) of coumarins. The evaluation of EC(50) of a single compound takes only 15 to 30 min. The structure-activity relationships of tested compounds are also established. The results verified 7,8-dihydroxy-4-methylcoumarins as excellent DPPH radical scavengers. Obtained results correspond with those of other studies and suggest the SIA procedure as a suitable method for fast and sensitive antioxidant analysis of various types of compounds.
- Publikační typ
- časopisecké články MeSH
Antioxidants perform an important role in the maintenance of the integrity of the living organisms. Recently, great attention has been given to antioxidants by a reason of their medical use. It is due to the association of many human diseases with oxidative stress. The present study briefly surveys the types of antioxidants, reactive oxygen and nitrogen species and describes the basic mechanisms of their activity. The application of antioxidants in the therapy of the injury caused by sulfur mustard, a vesicant type chemical warfare agent introduced in World War I, is also given.
- MeSH
- antioxidancia metabolismus terapeutické užití MeSH
- chemické bojové látky otrava MeSH
- lidé MeSH
- oxidační stres MeSH
- volné radikály metabolismus MeSH
- yperit otrava MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- antioxidancia MeSH
- chemické bojové látky MeSH
- volné radikály MeSH
- yperit MeSH
The thiophene polyine (E)-2-[5-(hept-5-en-1,3-diynyl)-thien-2-yl]-ethan-1,2-diol was isolated from an ethanolic extract of underground parts of Leuzea carthamoides. The thiophene polyine demonstrated significant antifungal activity against all tested species.
- MeSH
- Absidia účinky léků MeSH
- antifungální látky aplikace a dávkování farmakologie terapeutické užití MeSH
- Aspergillus účinky léků MeSH
- Asteraceae * MeSH
- Candida účinky léků MeSH
- fytoterapie * MeSH
- lidé MeSH
- mikrobiální testy citlivosti MeSH
- rostlinné extrakty aplikace a dávkování farmakologie terapeutické užití MeSH
- thiofeny aplikace a dávkování farmakologie terapeutické užití MeSH
- Trichophyton účinky léků MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antifungální látky MeSH
- rostlinné extrakty MeSH
- thiofeny MeSH
At the beginning of a new millennium, several important questions have come to the fore connected with the utilization of natural resources for the investigation of new medicaments (necessity of cooperation of both highly developed and developing countries in the protection of natural resources, necessity to justly evaluate and reward the contributions of the individual cultures in the case that a knowledge of traditional medicine was employed in the development of a new medicament, necessity to achieve the maximal chemical variety of real and virtual libraries of substances from which potential drugs are selected for screening tests). The research of chalcones and its analogues which are relatively easily available not only by isolation from natural material, but also by the methods of classical and combinatorial synthesis should not be substantially affected by the above-described changes. The present communication links up with previous papers (Opletalová, V., Sedivý, D.: Ces. slov. Farm. 48, 252 (1999); Opletalová, V.: Ces. slov. Farm. 49, 278 (2000); Opletalová, V. et al.: Folia Pharm. Univ. Carol. 25, 21 (2000)) and is devoted to the stabilizing action of chalcones on the vascular wall, vasodilating and anti-aggregating effects, and a favourable effect of their antioxidative activity on the functions of the cardiovascular system. The introduction also briefly discusses the nomenclature of this group of compounds.
- MeSH
- chalkon analogy a deriváty chemie terapeutické užití MeSH
- kardiovaskulární látky chemie terapeutické užití MeSH
- kardiovaskulární nemoci farmakoterapie MeSH
- kardiovaskulární systém účinky léků MeSH
- lidé MeSH
- zvířata MeSH
- Check Tag
- lidé MeSH
- zvířata MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- komentáře MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- chalkon MeSH
- kardiovaskulární látky MeSH
Extracts from the fruits (seeds) of Schisandra chinensis L. and pure isolated substances are one of the components of medicinal preparations designed for the treatment of cardiovascular diseases, liver diseases, diseases of the CNS related to the old age, as a supplement in the treatment of neoplasms, diabetes, etc. They are also used for the production of nutraceuticals (soft drinks and health foods), preparations for oral hygiene and for the care for the skin and hair. The review discusses complex analytical methods used for the determination of the content of substances and the phenomena of population ecology in relation with drug production.
- MeSH
- cyklooktany * MeSH
- kardiovaskulární nemoci farmakoterapie MeSH
- léčivé rostliny * MeSH
- lidé MeSH
- lignany * MeSH
- polycyklické sloučeniny * MeSH
- rostlinné extrakty izolace a purifikace terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cyklooktany * MeSH
- lignany * MeSH
- polycyklické sloučeniny * MeSH
- rostlinné extrakty MeSH
- schizandrin MeSH Prohlížeč
Schisandra chinensis (TURCZ.) BAILL., originally a Japanese-Manchurian endemite, yields a vegetable drug (Schisandrae fructus) with a number of very utilizable therapeutic effects. The paper reports the results of phytochemical and pharmacological-toxicological studies approximately from the year 1990 carried out both with the drug and, in particular, the principal isolated lignans of the dibenzo[a,c]cyclooctadiene type. The results confirm the validity of the historical use of the drug, in particular as a hepatoprotective, adaptogenic, and antioxidative agent. It is obvious that a very positive therapeutic effect based on the use of a complex mixture of its principal constituents because their biological effects are complementary and potentiate each other. At the same time, some lignans (e.g. gomisin A, gomisin N) are interesting as new prospective medicines.
- MeSH
- cyklooktany * MeSH
- kardiovaskulární nemoci farmakoterapie MeSH
- léčivé rostliny chemie MeSH
- lidé MeSH
- lignany chemie MeSH
- polycyklické sloučeniny chemie MeSH
- rostlinné extrakty farmakologie terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- cyklooktany * MeSH
- lignany MeSH
- polycyklické sloučeniny MeSH
- rostlinné extrakty MeSH
- schizandrin MeSH Prohlížeč
A reversed-phase high-performance liquid chromatographic (RP-HPLC) separation method with UV spectrophotometric detection has been developed for the determination of major components in leaves and caulomas of Schisandra chinensis Baill. The flavonols (quercetin and kaempferol) and (E)-cinnamic acid were analysed after extraction with alcohol from the dry plant material. Identification was based on retention times and UV spectra by comparison with commercial standards. Quercetin, kaempferol and (E)-cinnamic acid were separated within 12 min using acetonitrile-aqueous 0.05% ortho-phosphoric acid (40:60 v/v) mobile phase. The method has been successfully applied for the quantitative analysis of all three major components in several samples from different harvests using propylparaben as the internal standard.
- MeSH
- cinnamáty analýza MeSH
- flavonoidy * MeSH
- kempferoly * MeSH
- Magnoliopsida chemie MeSH
- quercetin analogy a deriváty analýza MeSH
- referenční standardy MeSH
- vysokoúčinná kapalinová chromatografie metody MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- cinnamáty MeSH
- cinnamic acid MeSH Prohlížeč
- flavonoidy * MeSH
- kaempferol MeSH Prohlížeč
- kempferoly * MeSH
- quercetin MeSH
20-Hydroxyecdysone (20-OH) is a natural compound with many demonstrated effects on the physiological functions of vertebrates, particularly increased protein synthesis. Our study sought a suitable dosage form with continuous release of the drug lasting several weeks for implantation into agricultural animals. Biodegradable microparticles and implants of poly(L-lactic) and poly(DL-lactic) acids were prepared. Oligomers of these materials were synthesized, and a method of melting the binary mixture of the oligomer and 20-OH was employed. The particles were prepared simply by grinding the solidified block of the melt and sieving. Implants were prepared by extruding the melt into silicone tubes, removing the solidified content, and cutting into cylinders of 2 mm diameter and various lengths. A new method of preparation of hollow cylinders by aspirating air into silicone tubes filled with the melt was developed. The experiments demonstrated stability of 20-OH during heat treatment. Release of the active ingredient was tested in static in vitro conditions, analogous to those at the site of implantation, and prolonged drug release was obtained with both types of implant. The hollow implants gave release rates nearest to ideal zero-order kinetics and would appear most appropriate for testing in vivo.
- MeSH
- ekdysteron farmakokinetika MeSH
- hospodářská zvířata * MeSH
- kinetika MeSH
- silikony MeSH
- vstřebatelné implantáty MeSH
- vysoká teplota MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- ekdysteron MeSH
- silikony MeSH
