Potatoes (Solanum tuberosum L.) are a significant food crop cultivated around the world. Caffeic acid (CA) can enhance plant growth by promoting antioxidant activity and stimulating root development, contributing to overall plant health and vigor. Cobalt sulfate (CoSO4) boosts plant growth by promoting nitrogen (N) fixation, healthier root development, and chlorophyll synthesis, enhancing photosynthesis and overall plant health. Nanoparticle-coated urea (NPCU) improves nutrient uptake, promoting plant growth efficiency and reducing environmental impact. This study investigates the effects of combining CA, CoSO4, and NPCU as amendments on potatoes with and without NPCU. Four treatments, control, 20 μM CA, 0.15 mg/L CoSO4, and 20 μM CA + 0.15 mg/L CoSO4 with and without NPCU, were applied in four replications using a completely randomized design. Results demonstrate that the combination of CA + CoSO4 with NPCU led to an increase in potato stem length (~ 6%), shoot dry weight (~ 15%), root dry weight (~ 9%), and leaf dry weight (~ 49%) compared to the control in nutrient stress. There was a significant rise in chlorophyll a (~ 27%), chlorophyll b (~ 37%), and total chlorophyll (~ 28%) over the control under nutrient stress also showed the potential of CA + CoSO4 with NPCU. In conclusion, the findings suggest that applying CA + CoSO4 with NPCU is a strategy for alleviating potato nutrient stress.
- Klíčová slova
- Solanum tuberosum L., Chlorophyll content, Growth attributes, N-fertilizer,
- MeSH
- chlorofyl metabolismus MeSH
- fotosyntéza účinky léků MeSH
- kobalt farmakologie chemie MeSH
- kořeny rostlin účinky léků růst a vývoj MeSH
- kyseliny kávové * farmakologie chemie MeSH
- listy rostlin účinky léků MeSH
- močovina * farmakologie MeSH
- nanočástice * chemie MeSH
- Solanum tuberosum * účinky léků růst a vývoj MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- caffeic acid MeSH Prohlížeč
- chlorofyl MeSH
- kobalt MeSH
- kyseliny kávové * MeSH
- močovina * MeSH
Bacterial spot, caused by a group of Xanthomonads (Xanthomonas spp.), is a devastating disease. It can adversely affect the Capsicum annum productivity. Scientists are working on the role of antioxidants to meet this challenge. However, research is lacking on the role of antioxidant enzymes and their isoforms in the non-compatible pathogen and host plant interaction and resistance mechanisms in capsicum varieties. The present study was conducted to ascertain the defensive role of antioxidant enzymes and their isoforms in chilli varieties Hybrid, Desi, Serrano, Padron, and Shehzadi against bacterial spot disease-induced Xanthomonas sp. The seedlings were inoculated with bacterial pathogen @ 107 CFU/mL, and samples were harvested after regular intervals of 24 h for 4 days followed by inoculation. Total plant proteins were extracted in phosphate buffer and quantified through Bradford assay. The crude protein extracts were analyzed through quantitative enzymatic assays in order to document activity levels of various antioxidant enzymes, including peroxidase (POD), Catalase (CAT), Ascorbate peroxidase (APX), and Superoxide dismutase (SOD). Moreover, the profiles appearance of these enzymes and their isoforms were determined using native polyacrylamide gel electrophoresis (PAGE) analysis. These enzymes exhibited maximum activity in Hybrid (HiR) cultivar followed by Desi (R), Serrano (S), Padron, and Shehzadi (HS). Both the number of isoforms and expression levels were higher in highly resistant cultivars compared to susceptible and highly susceptible cultivars. The induction of POD, CAT, and SOD occurs at the early stages of growth in resistant Capsicum cultivars. At the same time, APX seems to make the second line of antioxidant defense mechanisms. We found that modulating antioxidant enzymes and isoforms activity at the seedling stage was an important mechanism for mitigating plant growth inhibition in the resistant ones.
- MeSH
- antioxidancia metabolismus MeSH
- askorbátperoxidasa metabolismus MeSH
- Capsicum růst a vývoj mikrobiologie MeSH
- fyziologický stres fyziologie MeSH
- katalasa metabolismus MeSH
- nemoci rostlin mikrobiologie MeSH
- oxidační stres fyziologie MeSH
- peroxid vodíku analýza MeSH
- peroxidasa metabolismus MeSH
- protein - isoformy metabolismus MeSH
- rostlinné proteiny analýza MeSH
- superoxiddismutasa metabolismus MeSH
- Xanthomonas patogenita MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- antioxidancia MeSH
- askorbátperoxidasa MeSH
- katalasa MeSH
- peroxid vodíku MeSH
- peroxidasa MeSH
- protein - isoformy MeSH
- rostlinné proteiny MeSH
- superoxiddismutasa MeSH
