- MeSH
- bicyklické sloučeniny heterocyklické aplikace a dávkování MeSH
- hematologické nádory patologie terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfoproliferativní nemoci patologie terapie MeSH
- převzatá imunita * MeSH
- sulfonamidy aplikace a dávkování MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- Publikační typ
- kazuistiky MeSH
- práce podpořená grantem MeSH
- úvodníky MeSH
- Názvy látek
- bicyklické sloučeniny heterocyklické MeSH
- sulfonamidy MeSH
- venetoclax MeSH Prohlížeč
Chemotherapy combinations with monoclonal antibodies are now the basis for treatment of chronic lymphocytic leukemia. Rituximab, the most widely used anti-CD20 antibody in routine clinical practice, led not only to improvement of progression-free survival, but also to improvement of overall survival in previously untreated patients with good performance status in combination with fludarabine and cyclophosphamide. This regimen has become the standard treatment for patients in good physical condition. Rituximab and the newest anti-CD20 antibody obinutuzumab in combination with chlorambucil, as compared with chlorambucil alone, prolonged overall survival in previously untreated patients with significant comorbidities, and the combination of anti-CD20 antibody with chlorambucil has become the standard regimen in this group of patients. Alemtuzumab and ofatumumab improved treatment results in refractory chronic lymphocytic leukemia. Targeted therapy with combination chemotherapy and monoclonal antibody in patients with chronic lymphocytic leukemia represents a significant advance in the treatment of this disease.
- MeSH
- chronická lymfatická leukemie farmakoterapie mortalita MeSH
- lidé MeSH
- monoklonální protilátky terapeutické užití MeSH
- rituximab terapeutické užití MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
- Názvy látek
- monoklonální protilátky MeSH
- rituximab MeSH
Magnesium as a component of a range of enzymatic systems is a very important intracellular cation in the organism. Its monitoring is limited in many observations only to determination of its concentration in blood serum. We have done an analysis of serum and erythrocyte magnesium concentrations in 23 healthy women, 70 healthy men and in 184 patients with ulcerative disease of gastroduodenum. It was proved in all the monitored groups that erythrocyte concentrations of magnesium were lower compared to values which were estimated from serum concentrations in 9 from 23 healthy women, in 3 from 60 healthy men, in 10 from 101 ill men with ulcerative gastroduodenal disease, and in 17 from 83 women with ulcerative gastroduodenal disease. The highest rate of low concentrations of erythrocyte magnesium in healthy women with physiologic concentrations of magnesium in serum was also confirmed by currently created subgroup of healthy women (n = 11) who undergone together with other analysis peroral Mg2+ load test. This test confirmed magnesium deficiency in 10 from 11 women. The results showed there are more frequent deficiencies of magnesium in organisms then it is generally assumed. They also proved the importance of nutrition and regular food in population of healthy, young women.
- MeSH
- dospělí MeSH
- erytrocyty chemie MeSH
- hořčík krev MeSH
- lidé středního věku MeSH
- lidé MeSH
- nedostatek hořčíku komplikace diagnóza MeSH
- peptický vřed komplikace MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- hořčík MeSH
The authors evaluated retrospectively in a group of 69 adult patients with Hodgkin's lymphoma the relationship between the beta-2-microglobulin serum level, basic demographic parameters (age, sex) and factors characterizing the extent (stage III and IV, "bulk" or mediastinal mass, number of affected areas of lymph nodes) and activity of the tumour (presence of B-symptoms, red cell sedimentation rate, haemoglobin, albumin and lactate dehydrogenase level, number of leucocytes and lymphocytes). They analyzed also the possible prognostic impact of beta-2-microglobulin on the therapeutic response risk of relapse and patient's survival. Methods of univariant statistical analysis confirmed the correlation of beta-2-microglobulin level with all investigated metric parameters of patients (advanced age, number of affected nodes, red cell sedimentation rate and lactate dehydrogenase level, lower albumin, haemoglobin level, numbers of leucocytes and lymphocytes). In multivariant analysis however the only independent metric markers significantly associated with an elevated protein level were more advanced age of the patients (P = 0.0002) and a lower number of leucocytes (P = 0.05). The values of beta-2-microglobulin was not influenced by the extent of the tumour (stage III and IV, "bulk" or mediastinal mass, higher number of affected areas of lymph nodes). Significantly more frequently elevated protein values were recorded in patients with manifestations of B symptoms associated with the diagnosis (P = 0.0003). Multivariant analysis did not prove the importance of the serum level of beta-2-microglobulin as a prognostic factor in the sense of predicted remission, development of a relapse or death in conjunction with progression of Hodgkin's lymphoma.
- MeSH
- beta-2-mikroglobulin krev MeSH
- dospělí MeSH
- Hodgkinova nemoc krev patologie terapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfatické metastázy MeSH
- mladiství MeSH
- nádorové biomarkery krev MeSH
- počet leukocytů MeSH
- prognóza MeSH
- retrospektivní studie MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- beta-2-mikroglobulin MeSH
- nádorové biomarkery MeSH
In diabetic patients with ischaemia of the lower extremities and mediocalcinosis the diagnosis of ischaemia is still difficult. One of the methods which give an objective idea of the microcirculation is transcutaneous oximetry. In the submitted paper the authors explain the principle and problems of assessment with recommendations for the practical application of the method, in particular in indication for angiography of the lower extremities in the presence of the diabetic foot syndrome with regard to probable spontaneous healing of defects and application of the method for following up the effect of revascularization operations.
BACKGROUND: B-chronic lymphocytic leukemia (B-CLL), the most common type of leukemia in Western Europe and the United States, is characterized by clonal chromosomal abnormalities detected in almost half of the studied patients. The precise determination of chromosomal changes helps to indicate the prognosis and to understand the pathogenesis of CLL. METHODS AND PATIENTS: We applied conventional cytogenetics (CC), FISH and comparative genomic hybridization (CGH) to the investigation of clonal abnormalities in 88 B-CLL patients at the time of diagnosis. RESULTS: By using CC of bone marrow cells without any stimulation, non-random chromosomal changes were found in 17 (19%) of 88 patients. The employment of FISH and CGH revealed chromosomal changes in additional 33 patients, thus increasing the detection rate of chromosomal abnormalities to 57%. The most common abnormalities detected in our patients included deletions of 13q in 16 cases (18%), followed by trisomy of chromosome 12 in 12 patients (13%), deletions of 11q in 10 patients (11%) and deletions of 17p in 10 patients (11%). A statistically significant correlation between higher disease activity and the presence of deletions 11q and 17p was observed. CONCLUSION: The addition of FISH and CGH to CC in 88 B-CLL patients improved the detection of clonal chromosomal changes from 19 to 57%. The most frequent chromosomal change was deletion of 13q14 (18%). Deletions of 11q23 and 17p13 were found in patients with higher clinical disease activity. Our results underline the importance of employing FISH and CGH techniques in CLL patients. CC without any stimulation has a low detection rate and is not suggested for detection of chromosomal changes in CLL.
- MeSH
- chromozomální aberace genetika MeSH
- chromozomální delece MeSH
- chromozomální poruchy MeSH
- chronická lymfatická leukemie genetika MeSH
- hybridizace in situ fluorescenční * MeSH
- hybridizace nukleových kyselin * MeSH
- lidé středního věku MeSH
- lidé MeSH
- lidské chromozomy, pár 11 MeSH
- lidské chromozomy, pár 13 MeSH
- prediktivní hodnota testů MeSH
- prognóza MeSH
- senioři MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
In 23 patients, percutaneous atherectomy was performed with an endomyocardial biopsy device in 27 eccentric stenoses of lower limb arteries. The mean percentage stenosis was reduced from 82% to 14% (P: <.001). Histopathologic examination revealed no media or adventitial tissue in the extracted particles. The technical success and complication rates were similar to those reported for conventional percutaneous transluminal angioplasty of short symmetric lesions.
- MeSH
- arteriální okluzní nemoci diagnostické zobrazování terapie MeSH
- aterektomie škodlivé účinky přístrojové vybavení metody MeSH
- bérec krevní zásobení MeSH
- biopsie přístrojové vybavení MeSH
- digitální subtrakční angiografie MeSH
- endokard patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- senioři MeSH
- vybavení k jednorázovému použití MeSH
- Check Tag
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
BACKGROUND: We applied classical cytogenetics, FISH and CGH to investigate prognostic important chromosomal changes, deletions of 17p, 11q and trisomy of chromosome 12 in 90 B-CLL patients at the time of diagnosis. METHODS AND RESULTS: Using classical cytogenetics the chromosomal changes were detected in 17 (18%) patients. Trisomy 12 was found in three patients, deletion 11q in two patients and deletion 17p in four patients. The employment of FISH and CGH revealed chromosomal changes in 52 (58%) patients, the trisomy of chromosome 12 was detected in 12 (13%) patients, the deletions of 11q and deletions of 17p in 10 patients (11%). Statistically significant correlation between higher disease activity and the stage of disease and the presence of deletion 11q and 17p was found. The trisomy of chromosome 12 was found in patients with abnormal markers and in patients with higher stage of the disease. CONCLUSIONS: According to our results, the majority of chromosomal abnormalities in B-CLL may escape detection when classical cytogenetics is the only diagnostic technique used. It stresses the importance of employing additional techniques including FISH and CGH at the time of diagnosis.
- MeSH
- chromozomální aberace * MeSH
- chronická lymfatická leukemie genetika MeSH
- cytogenetické vyšetření MeSH
- DNA nádorová genetika MeSH
- dospělí MeSH
- hybridizace in situ fluorescenční MeSH
- hybridizace nukleových kyselin MeSH
- lidé středního věku MeSH
- lidé MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- DNA nádorová MeSH
BACKGROUND: The aim of the work was to find out whether the controlled administration of a thrombolytic directly into the arterial occlusion could prevent formation of the systemic lytic state, which may result in distant bleeding complications. METHODS AND RESULTS: In patients subjected to pulsatile pharmacomechanical (94 patients) and local continual thrombolysis (74 patients) changes in blood and plasma viscosity, coagulation tests, fibrinogen levels and some other parameters were investigated. CONCLUSIONS: Pulsatile pharmacomechanical thrombolysis, similarly to other forms of thrombolysis, is accompanied with systemic lytic state and the risk of unwanted resorption of the hemostatic thrombosis. Urokinase administration brings about regress of systemic changes, which is similar to the use of either tested thrombolytic administration. As treatment by the continual thrombolysis takes is approximately 40 times longer, the risk of systemic lytic state and bleeding complication growths.
- MeSH
- aktivátor plazminogenu urokinázového typu aplikace a dávkování MeSH
- aktivátory plazminogenu aplikace a dávkování MeSH
- bérec krevní zásobení MeSH
- dospělí MeSH
- hemokoagulace MeSH
- ischemie farmakoterapie MeSH
- lidé středního věku MeSH
- lidé MeSH
- periferní katetrizace MeSH
- pulzatilní průtok MeSH
- reologie krve * MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- trombolytická terapie metody MeSH
- trombóza krev farmakoterapie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- aktivátor plazminogenu urokinázového typu MeSH
- aktivátory plazminogenu MeSH
BACKGROUND: The blood viscosity is one of the factors affecting tissue blood flow. Aim of the study was to establish how the blood viscosity and internal viscosity of the red cell (Tk) may be influenced by change of the temperature. METHODS AND RESULTS: We have measured plasma and blood viscosity in 20 samples of human plasma and 17 samples of human blood. Viscosity was established with rotational viscometer under the temperature ranging from 25 degrees C to 45 degrees C. Concomitantly the internal viscosity of the red cell (Tk) was estimated. Plasma and blood viscosities are decreasing with the temperature elevation but the change is not linear through temperature range used for testing. The most pronounced change was found in relatively lower temperatures. Relative viscosity of the blood did not change significantly with the temperature except of measurements under low shear conditions performed in low temperature. Relative blood viscosity increased with temperature fall in such a situation. The internal viscosity of the red cell (Tk) increased significantly with temperature elevation. CONCLUSIONS: The average values of the blood and plasma viscosity fell with temperature elevation but the change was not linear. The plasma viscosity change was the highest in the low temperature range. These findings together with significant differences between relative and dynamic blood viscosity in low shear conditions and in 25 degrees C showed that blood viscosity was influenced by qualitative changes of the plasma followed by higher aggregation of the red cells.
- MeSH
- erytrocyty fyziologie MeSH
- lidé MeSH
- reologie krve * MeSH
- techniky in vitro MeSH
- teplota * MeSH
- viskozita krve * MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
