We investigated the expression of cell-associated CAIX protein in histological sections of the transitional cell carcinoma (TCC) of the urinary tract and of the soluble form of CAIX (s-CAIX) shed by the tumor into the serum and urine of TCC patients. A total of 23 patients with histologically confirmed TCC or squamous cell carcinoma (SCC) were enrolled in the pilot study. Sixteen healthy individuals served as controls. Membrane-bound CAIX was present in the tumor cells near the endoluminal surface. Necrosis was observed in only 4 samples. Using Western blots, s-CAIX concentrated from urine was visualized as a double band at 50 and 54 kDa. In most cases, the presence of s-CAIX in the urine correlated with CAIX expression in the tumor. On the other hand, s-CAIX did not exceed the normal level in the serum of TCC patients. Urine from patients with TCC of the urinary bladder and renal pelvis contained s-CAIX, allowing the detection of tumors in approximately 70% of the patients. Moreover, two additional patients with suspected, but unconfirmed bladder tumor, with s-CAIX detected in urine, developed tumors identified as TCC within six months. We suggest that after a simple, rapid and sensitive test, monitoring s-CAIX levels in urine will be developed, it may be useful for early detection of relapse in patients following transurethral tumor resection.
- MeSH
- antigeny nádorové metabolismus MeSH
- buněčná membrána enzymologie patologie MeSH
- dospělí MeSH
- ELISA MeSH
- imunoenzymatické techniky MeSH
- karboanhydrasa IX MeSH
- karboanhydrasy metabolismus MeSH
- karcinom z přechodných buněk krev enzymologie moč MeSH
- ledvinná pánvička enzymologie patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- močový měchýř enzymologie patologie MeSH
- nádorové biomarkery metabolismus MeSH
- pilotní projekty MeSH
- prognóza MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- staging nádorů MeSH
- studie případů a kontrol MeSH
- urologické nádory krev enzymologie moč MeSH
- western blotting MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- antigeny nádorové MeSH
- CA9 protein, human MeSH Prohlížeč
- karboanhydrasa IX MeSH
- karboanhydrasy MeSH
- nádorové biomarkery MeSH
Tumour-associated protein carbonic anhydrase IX (CA IX) has two major forms. One is a cell-associated, transmembrane protein seen on Western blots as a twin band of 54/58 kDa, expressed in gastric mucosa and in several types of cancer. The other is a soluble protein s-CA IX of 50/54 kDa, which is released into the culture medium or into the body fluids, most likely by proteolytic cleavage of the extracellular part from transmembrane and intracellular sequences. While TC media of CA IX-positive tumour cell lines or short-term cultures of tumour explants contain a relatively high concentration of s-CA IX (20-50 ng ml(-1)), the level of this antigen in blood serum and urine of renal clear cell carcinoma patients is about 1000 x lower. The concentration of CA IX in the blood and in urine varies within wide limits and there is no obvious correlation with tumour size. After nephrectomy, s-CA IX is cleared from the blood within a few days. Only an extremely low concentration of CA IX was detectable in the sera and in urine of control individuals.
- MeSH
- antigeny nádorové krev moč MeSH
- buněčná membrána metabolismus MeSH
- dospělí MeSH
- ELISA MeSH
- karboanhydrasa IX MeSH
- karboanhydrasy krev moč MeSH
- karcinom z renálních buněk enzymologie metabolismus patologie MeSH
- lidé středního věku MeSH
- lidé MeSH
- nádorové biomarkery krev moč MeSH
- nádorové buňky kultivované MeSH
- nádorové proteiny krev moč MeSH
- nádory ledvin enzymologie metabolismus patologie MeSH
- northern blotting MeSH
- prognóza MeSH
- senioři MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- antigeny nádorové MeSH
- CA9 protein, human MeSH Prohlížeč
- karboanhydrasa IX MeSH
- karboanhydrasy MeSH
- nádorové biomarkery MeSH
- nádorové proteiny MeSH
MN/CA IX is a cell surface protein, strongly associated with several types of human carcinomas. It exerts activity of carbonic anhydrase and capacity of binding to cell surface receptors. In the present work, we used affinity purified MN/CA IX protein to demonstrate that the cells adhere to immobilized MN/CA IX and that the monoclonal antibody M75 abrogates cell attachment to MN/CA IX. Using synthetic oligopeptides, we identified M75 epitope and located it in the proteoglycan domain, which contains a sixfold tandem repeat of six amino acids GEEDLP. From phage display library of random heptapeptides we identified and chemically synthesized those which compete for the epitope with M75 and inhibit adhesion of cells to MN/CA IX. These heptapeptides might serve as lead compounds for drug design.
- MeSH
- buněčná adheze * MeSH
- chromatografie afinitní MeSH
- ELISA MeSH
- lidé MeSH
- mapování epitopu * MeSH
- molekulární sekvence - údaje MeSH
- molekuly buněčné adheze chemie imunologie metabolismus MeSH
- nádorové buňky kultivované MeSH
- nádorové proteiny chemie imunologie metabolismus MeSH
- sekvence aminokyselin MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- molekuly buněčné adheze MeSH
- nádorové proteiny MeSH
The MN protein is associated with certain human carcinomas, but absent in most normal tissues. It is a transmembrane protein; its extracellular part contains a domain homologous with carbonic anhydrases (CAs) and a proteoglycan-like region. In the present study, we observed that cells (human CGL1 and mouse NIH3T3 cells) transfected with MN cDNA showed morphologic transformation, but reverted to normal phenotype after 4-5 weeks. This reversion was not due to the loss, silencing, or mutations of MN insert. We also found that MN protein exerted CA enzymatic activity, but this was not relevant for morphologic transformation of cells. MN is an adhesion protein, involved in cell-to-cell contacts, this probably could explain its role in tumorigenesis.
- Publikační typ
- časopisecké články MeSH
MaTu is an agent, believed to be derived from a human mammary carcinoma, which displayed several extraordinary properties. These were: RIP and PAGE revealed in MaTu-infected cells only a single protein band of Mr 58 k, the gp 58. This gp 58 was immunoprecipitated by antibodies present in some human sera as well as in some sera of rabbits, sheep, and cattle. MaTu had an extremely restricted host range: it was transmissible only to HeLa cells, but not to human embryo fibroblasts, to three human tumour cell lines (T 47 D, T 24, and HMB 2) or to monkey Vero and rabbit SIRC cells. A retrovirus with a broad host range, used as a helper (X-MLV) enabled the transmission of MaTu to human fibroblasts, but not to Vero or SIRC, which are also permissive for X-MLV. These observations, together with our previous reports, support the view that MaTu might either be a novel type of defective virus, or even a non-viral autonomous genetic element.
- MeSH
- antisérum MeSH
- defektní viry analýza izolace a purifikace patogenita MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- HeLa buňky MeSH
- králíci MeSH
- lidé MeSH
- nádorové buňky kultivované MeSH
- ovce MeSH
- pomocné viry MeSH
- precipitinové testy MeSH
- protilátky virové krev imunologie MeSH
- Retroviridae analýza izolace a purifikace patogenita MeSH
- virové proteiny analýza MeSH
- virus myší leukemie MeSH
- virus vezikulární stomatitidy, kmen Indiana * analýza izolace a purifikace patogenita MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- lidé MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antisérum MeSH
- protilátky virové MeSH
- virové proteiny MeSH
RHV, which is presumably a defective human retrovirus, has been recovered from the human melanoma cell line HMB2. In the presence of Moloney mouse leukemia virus (MLV), used as a helper, RHV is serially transmissible in mouse NIH-3T3 cells and can provide envelope antigens for vesicular stomatitis virus pseudotype--VSV(RHV). This pseudotype is neutralizable with an inhibitor, present in all human sera tested; the inhibitory activity is resistant to heating at 100 degrees C. To detect whether any specific response to RHV is connected with the disease, we isolated IgG from 140 human sera (70 melanoma patients and 70 control group donors) and tested it for neutralization of VSV(RHV) pseudotype in a randomized, blind experiment. Sixteen samples of IgG from the sera of melanoma patients, but only one control IgG, neutralized the pseudotype; this difference was significant (p less than 0.001). RHV thus appears to be in some way associated with melanoma.
- MeSH
- defektní viry imunologie izolace a purifikace MeSH
- imunoglobulin G imunologie MeSH
- lidé MeSH
- melanom etiologie imunologie MeSH
- neutralizační testy MeSH
- Retroviridae imunologie izolace a purifikace MeSH
- vysoká teplota MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- imunoglobulin G MeSH
- MeSH
- Escherichia coli účinky léků MeSH
- kolifágy analýza MeSH
- teoretické modely MeSH
- virová interference * MeSH
- Publikační typ
- časopisecké články MeSH
