AIM: Pinworm infection (known as enterobiasis or oxyuriasis) is one of the most common parasitic diseases globally and in the Czech Republic (CZ). The aim of this study is to analyse the available epidemiological data on the incidence of enterobiasis in the CZ from 2018-2022. METHODS: A descriptive analysis was done of enterobiasis (ICD-10 code B80) data reported to the electronic Infectious Disease Information System in the CZ from 2018 to 2022. Data processing and analysis were conducted using MS Excel 2016. Univariate and multivariate logistic regression analyses were performed to assess the association between the probability of hospitalization and categorical variables using STATA version 17. The ECDC Map Maker tool (EMMa) was used to create the incidence map. RESULTS: A total of 4,836 cases were reported during the study period, with an average annual incidence of 9.1 cases per 100,000 population. The highest number of cases occurred in 2019 (n = 1,174), and the lowest in 2021 (n = 780). The disease was most common in the paediatric population, with the highest average age-specific incidence rates observed in children aged 5-9 years (80.9 per 100,000 population) and 10-14 years (42.3 per 100,000 population). Of 14 administrative regions of the CZ, the Olomouc Region had the highest average annual incidence (28.7 per 100,000 population), while the Pilsen Region had the lowest (2.2 per 100,000 population). A total of 472 (9.8%) patients needed hospitalization, most of them in the categories 10-14 years (n = 200, 42.4%) and 5-9 years (n = 178, 38%). The highest hospitalization rate was found in the age group 75+ (36.4%). A significantly higher probability of hospitalization was found in the age groups 6-19 years and 65+ compared to working-age population with enterobiasis. A significantly lower probability of hospitalization was seen in 2020-2022 compared to 2019. No difference in the hospitalization rates was noted between genders. No enterobiasis-related death was reported during the study period. The disease occurs year-round. A decrease in reported cases was observed annually during the school summer holidays in July and August. Neither outbreak nor imported cases were noted. CONCLUSION: Given that enterobiasis is often asymptomatic, many cases are not captured in the surveillance system. The Czech prevalence data indicate that it mainly affects the paediatric population. Therefore, preventive measures and programs should primarily target children.
- Klíčová slova
- Enterobiasis – Enterobius vermicularis – oxyuriasis – parasitic diseases – epidemiology – children – Czech Republic – descriptive analysis,
- MeSH
- dítě MeSH
- dospělí MeSH
- enterobióza * epidemiologie MeSH
- hospitalizace statistika a číselné údaje MeSH
- incidence MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé středního věku MeSH
- lidé MeSH
- mladiství MeSH
- mladý dospělý MeSH
- mužské pohlaví MeSH
- novorozenec MeSH
- předškolní dítě MeSH
- senioři nad 80 let MeSH
- senioři MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Česká republika epidemiologie MeSH
This study was carried out to investigate how pinworm infection in rats affects nutrient digestibility in the hosts. Twenty-four male outbred Wistar rats were randomly divided into two groups of 12 rats each. The rats from the first group (GI) were kept in cages with bedding containing pinworm eggs, and the second (control) group (GII) were kept in a separate room in clean, uncontaminated filter-top cages. The animals were put into individual metabolic cages later. Metabolic trials lasted five days and records of animal weight, food ingestion, and faecal weight were taken daily. Based on laboratory analysis of the feed and faecal nutrient content, digestibility values were determined. On day 15 of the experiment, the animals were euthanized. Although Syphacia muris were found in all rats from the GI group, animals exhibited no clinical signs. In our experiment, S. muris infection reduced the overall digestibility of all measured nutrients (P < 0.01). The most significant differences in digestibility were observed in the case of crude fibre and mineral matter (P < 0.01).
- Klíčová slova
- Syphacia muris, digestibility, infection, laboratory rat, nutrient,
- MeSH
- energetický metabolismus MeSH
- fyziologie výživy zvířat * MeSH
- krysa rodu Rattus * MeSH
- laboratorní zvířata MeSH
- náhodné rozdělení MeSH
- nemoci hlodavců parazitologie MeSH
- oxyuriáza parazitologie MeSH
- Oxyuroidea fyziologie MeSH
- potkani Wistar MeSH
- stravovací zvyklosti MeSH
- trávení * MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus * MeSH
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
Extraintestinal oxyuriasis, in our experience with three affected women of fertile age, presented itself as a solitary fibrotic nodular lesion, with a varying location. The sites of location were: parietal peritoneum, serous surface of the uterus and wall of the uterine tube. The size of the nodules was 5 to 10 mm. Histologically, the lesions were hypocellular fibrotic nodules with a variable amount of neutrophils and amorphous eosinophilic material in the center, harbouring eggs of the parasite and remnants of pinworm cuticle. All three lesions were asymptomatic, only being discovered incidentally during the operations for unrelated conditions. Their peroperative recovery by a surgeon did not alter the course of surgery. These findings document the ability of pinworms to migrate into the abdominal cavity via the female genital tract.
- MeSH
- dospělí MeSH
- lidé MeSH
- náhodný nález MeSH
- oxyuriáza patologie MeSH
- peritoneum parazitologie MeSH
- uterus parazitologie MeSH
- vejcovody parazitologie MeSH
- Check Tag
- dospělí MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- přehledy MeSH
Pinworms (Nematoda: Enterobiinae) include 52 species parasitising primates throughout the world. In the present study, we performed the first ever molecular analysis to investigate the phylogenetic position of recently described pinworms parasitising the Sumatran orang-utan. The phylogenetic analysis of mitochondrial CO1 and chromosomal 18S rDNA and ITS1 regions could support the independent status of several Nematoda species. Our molecular data clearly suggest that Enterobius (Colobenterobius) buckleyi and Lemuricola (Protenterobius) pongoi together with Pongobius hugoti form separate clades among other studied species, which significantly supports the hypothesis of recently described new species parasitising the orang-utan (Pongo abelii and Pongo pygmaeus). The phylogenetic tree based on cytochrome oxidase 1 (CO1) gene variability showed possible close relationships between L. (Protenterobius) pongoi and P. hugoti; thus, we can assume that these species could have initially diverged in sympatry from a common ancestor.
- MeSH
- enterobióza parazitologie veterinární MeSH
- Enterobius klasifikace genetika MeSH
- fylogeneze * MeSH
- genetické markery MeSH
- intergenová DNA genetika MeSH
- mitochondrie genetika MeSH
- molekulární sekvence - údaje MeSH
- Pongo abelii parazitologie MeSH
- Pongo pygmaeus parazitologie MeSH
- respirační komplex IV genetika MeSH
- sekvence aminokyselin MeSH
- vznik druhů (genetika) MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- genetické markery MeSH
- intergenová DNA MeSH
- respirační komplex IV MeSH
Pinworms (Nematoda: Oxyurida) are common contaminants in most laboratory rodent colonies. The aim of the study was to monitor the transmission of Syphacia muris eggs in laboratory rat breeding facilities. Dust in a breeding room was investigated using special grids (free fallout, or through the help suction chamber). Furthermore, the ventilation system, breeding cages and the hands of the laboratory technical staff were examined. In the case of free fallout, the percentage of positive grids increased slightly over time: from 5.5% (after 24 h) to 8.2% (72 h). Similar values were also found when using the suction chamber (7.6%). Many more pinworm eggs were found in samples collected every second month from suction holes of the ventilation system (28.7%). One-half of the samples taken from the breeding cages (before washing) exhibited pinworm eggs (50.8%). Examination of the hands of technical staff showed positive detection in 37.9% of cases. In this study, certain transmission factors (dust, unclean cages and technicians) were proved to be significant in the distribution of pinworm infection in laboratory rodent facilities.
- MeSH
- bydlení zvířat * MeSH
- chov * MeSH
- enterobióza prevence a kontrola přenos veterinární MeSH
- kontaminace zdravotnického vybavení * MeSH
- krysa rodu Rattus parazitologie MeSH
- monitorování životního prostředí MeSH
- nemoci hlodavců parazitologie prevence a kontrola přenos MeSH
- ovum MeSH
- Oxyuroidea izolace a purifikace MeSH
- prach MeSH
- pracovníci laboratoře * MeSH
- větrání MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus parazitologie MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- prach MeSH
In this first report about pinworms peptidases we primarily characterize peptidases released during in vitro maintenance of two common pinworms of laboratory animals -Syphacia muris and Passalurus ambiguus. The peptidase activity obtained using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) showed the presence of peptidases from S. muris with a wide range of molecular size (25-110 kDa), which degrades gelatin and mucin at alkaline pH levels. P. ambiguus released serine and aspartyl peptidases degrading gelatin at all tested pH (3, 5, 7, and 9) and at acidic pH Passalurus released aspartyl and cysteine peptidases which are active against mucin.
- MeSH
- EDTA farmakologie MeSH
- elektroforéza v polyakrylamidovém gelu MeSH
- inhibitory proteas farmakologie MeSH
- interakce hostitele a parazita fyziologie MeSH
- koncentrace vodíkových iontů MeSH
- králíci MeSH
- krysa rodu Rattus MeSH
- laboratorní zvířata MeSH
- molekulová hmotnost MeSH
- muciny metabolismus MeSH
- oxyuriáza parazitologie MeSH
- Oxyuroidea enzymologie MeSH
- potkani Wistar MeSH
- proteasy chemie metabolismus MeSH
- substrátová specifita MeSH
- sulfony farmakologie MeSH
- želatina metabolismus MeSH
- zvířata MeSH
- Check Tag
- králíci MeSH
- krysa rodu Rattus MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- 4-(2-aminoethyl)benzenesulfonylfluoride MeSH Prohlížeč
- EDTA MeSH
- inhibitory proteas MeSH
- muciny MeSH
- proteasy MeSH
- sulfony MeSH
- želatina MeSH
The study presents the first description of male specimen (allotype) of the pinworm species Lemuricola (Lemuricola) pongoi, parasitising Sumatran orangutan (Pongo abelii) from Sumatra (Indonesia). The male specimen morphology presents all features fully corresponding with diagnose of the genus and subgenus Lemuricola. We are agreeing that Protenterobius is synonymous with Lemuricola because the length of tail appendix in males is not a sufficient feature for separation of the genera or subgenera. For L. (L.) pongoi male are characteristic features: body length (4,803 microm), total oesophagus length (530), tail length (235), tip of tail length (188) and spicule length (90).
- MeSH
- mikroskopie MeSH
- nemoci lidoopů parazitologie MeSH
- oxyuriáza parazitologie veterinární MeSH
- Oxyuroidea anatomie a histologie izolace a purifikace MeSH
- Pongo abelii parazitologie MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Indonésie MeSH
- MeSH
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- oxyuriáza farmakoterapie epidemiologie MeSH
- předškolní dítě MeSH
- ubytovací zařízení * MeSH
- zařízení denní péče pro děti * MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Československo epidemiologie MeSH
A total of 1,167 stool specimens collected from 0.6-6 years old patients attending King Abdel Aziz University Hospital (KAUH) in Riyadh, were examined for intestinal parasites. Of these 243 (20.8%) were positive. Giardia lamblia (13.5) and Enterobius vermicularis (4.2%), were the commonest parasites found. Other parasites present include Ascaris lumbricoides, Entamoeba histolytica and Hymenolepis nana. Abdominal pain (38.6%) and diarrhoea (27.6%) were the most common causes of referral presented among both males and females examined groups. Out of 211 patients positive for different parasites and showing different causes of referral, 45.5% were accompanied with abdominal pain and 22.3% having pruritus ani, while the percentage of patients having diarrhoea and positive for different parasites (9.5%) are less. It has been concluded that diarrhoea is not a major sign of parasitic infestation in 0.6-6 years old age group. Other causes of referral include, loss of appetite, underweight and failure to thrive which are mainly associated with Giardia lamblia infection.
- MeSH
- amébová dyzenterie epidemiologie MeSH
- askarióza epidemiologie MeSH
- giardiáza epidemiologie MeSH
- hymenolepiáza epidemiologie MeSH
- kojenec MeSH
- lidé MeSH
- oxyuriáza epidemiologie MeSH
- parazitární nemoci střev epidemiologie MeSH
- předškolní dítě MeSH
- sexuální faktory MeSH
- věkové faktory MeSH
- Check Tag
- kojenec MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Geografické názvy
- Saudská Arábie epidemiologie MeSH
- MeSH
- apendicitida diagnóza etiologie MeSH
- diferenciální diagnóza MeSH
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- oxyuriáza komplikace diagnóza MeSH
- předškolní dítě MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH