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MeSH: aftózní stomatitida-imunologie

6 záznamů v PubMed Filtry

Článek

Recurrent aphthous stomatitis and gene variability in selected interleukins: a case-control study

Borilova Linhartova, Petra
Autor Borilova Linhartova, Petra Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Janos, Julius
Autor Janos, Julius Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Slezakova, Simona
Autor Slezakova, Simona Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Bartova, Jirina
Autor Bartova, Jirina Institute of Clinical and Experimental Dental Medicine, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic
Petanova, Jitka
Autor Petanova, Jitka Department of Immunology and Microbiology, General University Hospital and First Faculty of Medicine, Charles University, Prague, Czech Republic
Kuklinek, Pavel
Autor Kuklinek, Pavel Department of Clinical Immunology and Allergology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Fassmann, Antonin
Autor Fassmann, Antonin Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic
Dusek, Ladislav
Autor Dusek, Ladislav Institute of Biostatistics and Analyses, Masaryk University, Brno, Czech Republic
Izakovicova Holla, Lydie
Autor Autorita ORCID Clinic of Stomatology, Institution Shared with St. Anne's Faculty Hospital, Faculty of Medicine, Masaryk University, Brno, Czech Republic Department of Pathophysiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic

European journal of oral sciences. 2018 Dec ; 126 (6) : 485-492. [epub] 20181020

Eur J Oral Sci
ISSN 1600-0722 | 0909-8836
Zdroj

Genetic factors, especially those related to immune system functioning, have been intensively studied to determine their role in the development of recurrent aphthous stomatitis (RAS). The aim of the present study was to analyze gene variability in interleukin (IL)2, IL4 (and its receptor α, IL4Rα), IL10, and IL13, which were selected based on literature review and/or their functional relevance, in Czech patients with RAS and in healthy controls. In total, 252 subjects (178 controls and 74 patients with RAS) were enrolled in this case-control study, and their detailed anamnestic, clinical, and laboratory data were obtained. Nine polymorphisms in the genes encoding interleukins were determined using PCR techniques. There were no significant differences in allele or genotype frequencies of the IL2, IL4, IL4Rα, IL10, and IL13 polymorphisms rs2069762/rs2069763, rs2243250/rs79071878, rs1801275, rs1800896, and rs1800925, respectively, between controls and patients with RAS. The minority alleles rs1800871 and rs1800872, which encode variants of IL10, were associated with a statistically significantly higher risk of RAS, as confirmed by the results of genotype and haplotype analyses. We suggest that variability in the IL10 gene may play an important role in the development of RAS in the Czech population.

Klíčová slova
association studies, candidate gene, genetic, immunity, oral ulcer, polymorphisms,
MeSH
aftózní stomatitida epidemiologie imunologie MeSH
alely MeSH
dospělí MeSH
frekvence genu MeSH
genetická predispozice k nemoci MeSH
genetická variace * MeSH
genotyp MeSH
haplotypy MeSH
interleukin-10 genetika metabolismus MeSH
interleukin-13 genetika metabolismus MeSH
interleukin-2 genetika MeSH
interleukin-4 genetika metabolismus MeSH
interleukiny genetika metabolismus MeSH
lidé středního věku MeSH
lidé MeSH
molekulární epidemiologie * MeSH
polymerázová řetězová reakce MeSH
polymorfismus genetický MeSH
receptor interleukinu-4 - alfa-podjednotka genetika metabolismus MeSH
studie případů a kontrol MeSH
Check Tag
dospělí MeSH
lidé středního věku MeSH
lidé MeSH
mužské pohlaví MeSH
ženské pohlaví MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Geografické názvy
Česká republika epidemiologie MeSH
Názvy látek
IL10 protein, human MeSH Prohlížeč
IL2 protein, human MeSH Prohlížeč
IL4 protein, human MeSH Prohlížeč
IL4R protein, human MeSH Prohlížeč
interleukin-10 MeSH
interleukin-13 MeSH
interleukin-2 MeSH
interleukin-4 MeSH
interleukiny MeSH
receptor interleukinu-4 - alfa-podjednotka MeSH

Článek

Polyclonal, newly derived T cells with low expression of inhibitory molecule PD-1 in tonsils define the phenotype of lymphocytes in children with Periodic Fever, Aphtous Stomatitis, Pharyngitis and Adenitis (PFAPA) syndrome

Molecular immunology. 2015 May ; 65 (1) : 139-47. [epub] 20150203

Mol Immunol
ISSN 1872-9142 | 0161-5890
Zdroj

PURPOSE: PFAPA syndrome is a benign, recurrent inflammatory disease of childhood. Tonsillectomy is one of the therapeutic options with a yet unexplained biological mechanism. We tested whether specific lymphocyte subsets recruited from blood to human tonsils participate in PFAPA pathogenesis. METHODS: Paired tonsils/peripheral blood (PB) samples were investigated (a) from children with PFAPA that successfully resolved after tonsillectomy (n=10) (b) from children with obstructive sleep apnoea syndrome as controls (n=10). The lymphocyte profiles were analysed using 8-colour flow cytometry, immunoglobulin (IGH) and T-cell receptor (TCR) gene rearrangements via PCR and next generation sequencing; a TREC/KREC analysis was performed using qPCR. RESULTS: The PFAPA tonsils in the asymptomatic phase had a lower percentage of B-lymphocytes than controls; T-lymphocyte counts were significantly higher in PB. The percentages of cytotoxic CD8pos T-lymphocytes were approximately 2-fold higher in PFAPA tonsils; the transitional B cells and naïve stages of both the CD4pos and CD8pos T-lymphocytes with a low expression of PD-1 molecule and high numbers of TREC were also increased. With the exception of elevated plasmablasts, no other differences were significant in PB. The expression levels of CXCL10, CXCL9 and CCL19 genes were significantly higher in PFAPA tonsils. The IGH/TCR pattern showed no clonal/oligoclonal expansion. DNA from the Epstein-Barr virus, Human Herpervirus-6 or adenovirus was detected in 7 of 10 PFAPA tonsils but also in 7 of 9 controls. CONCLUSIONS: Our findings suggest that the uninhibited, polyclonal response of newly derived lymphocytes participate in the pathogenesis of PFAPA. Because most of the observed changes were restricted to tonsils and were not present in PB, they partly explain the therapeutic success of tonsillectomy in PFAPA syndrome.