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15 záznamů v PubMed Filtry

Článek

Absorption kinetics of 5-aminosalicylic acid in rat: influence of indomethacin-induced gastrointestinal lesions and Escherichia Coli Nissle 1917 medication

Neuro endocrinology letters. 2011 ; 32 Suppl 1 () : 46-52.

Neuro Endocrinol Lett
ISSN 0172-780X
Zdroj

OBJECTIVES: The therapeutic effect of probiotics has been studied in many clinical and experimental studies but no data exist concerning the influence of probiotics on pharmacokinetics of contemporary administered drugs. In this paper, we describe the influence of indomethacin-induced gastrointestinal lesions and Escherichia Coli Nissle 1917 medication on absorption of 5-aminosalicylic acid and its metabolite N-acetyl-5-aminosalicylic acid in rat. METHODS: 5-aminosalicylic acid (5-ASA) was given orally to rat using gastric probe as a suspension (25 mg/kg). The plasma time profiles of 5-ASA and its metabolite were compared between Group A (animals medicated with a suspension of Escherichia coli Nissle 1917 [EcN] in dose of 5 × 108 CFUs/day for 14 consecutive days), Group B (animals with indomethacin [IND]-induced gastrointestinal lesions; single dose of 25 mg/kg of IND), Group C (simultaneous administration of EcN and IND), and Group D (control animals without any medication). The blood samples for HPLC analysis has been taken from incannulated vena jugularis in time 30, 60, 90, 120, 180, 240, 360 min after 5-ASA administration to rat. RESULTS: The pharmacokinetics of 5-ASA was not significantly changed by EcN medication (Group A) in comparison to control animals (Group D). The significantly elevated absorption (AUC and cmax) of 5-ASA was found in animals with induced gastro-enteropathy with concurrently medicated with EcN (Group C) when compred to controls. In the case of metabolite N-acetyl-5-ASA, statistically no-significant differences were found between groups. CONCLUSIONS: Simultaneous probiotics (EcN) medication did not affect absorption 5-ASA from intestinal tract (the main site of ASAs action).

MeSH
absorpce MeSH
antiflogistika nesteroidní škodlivé účinky farmakologie MeSH
Escherichia coli fyziologie MeSH
gastrointestinální nemoci chemicky indukované metabolismus MeSH
gastrointestinální trakt účinky léků metabolismus MeSH
indomethacin škodlivé účinky farmakologie MeSH
kinetika MeSH
krysa rodu Rattus MeSH
mesalamin krev farmakokinetika MeSH
osmolární koncentrace MeSH
potkani Wistar MeSH
probiotika farmakologie terapeutické užití MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
mužské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
hodnotící studie MeSH
práce podpořená grantem MeSH
Názvy látek
antiflogistika nesteroidní MeSH
indomethacin MeSH
mesalamin MeSH

Článek

Bacteriocinogeny in experimental pigs treated with indomethacin and Escherichia coli Nissle

World journal of gastroenterology. 2011 Feb 07 ; 17 (5) : 609-17.

World J Gastroenterol
ISSN 2219-2840 | 1007-9327
Zdroj

AIM: To evaluate bacteriocinogeny in short-term high-dose indomethacin administration with or without probiotic Escherichia coli Nissle 1917 (EcN) in experimental pigs. METHODS: Twenty-four pigs entered the study: Group A (controls), Group B (probiotics alone), Group C (indomethacin alone) and Group D (probiotics and indomethacin). EcN (3.5×10(10) bacteria/d for 14 d) and/or indomethacin (15 mg/kg per day for 10 d) were administrated orally. Anal smears before and smears from the small and large intestine were taken from all animals. Bacteriocin production was determined with 6 different indicator strains; all strains were polymerase chain reaction tested for the presence of 29 individual bacteriocin-encoding determinants. RESULTS: The general microbiota profile was rather uniform in all animals but there was a broad diversity in coliform bacteria (parallel genotypes A, B1, B2 and D found). In total, 637 bacterial strains were tested, mostly Escherichia coli (E. coli). There was a higher incidence of non-E. coli strains among samples taken from the jejunum and ileum compared to that of the colon and rectum indicating predominance of E. coli strains in the large intestine. Bacteriocinogeny was found in 24/77 (31%) before and in 155/560 (28%) isolated bacteria at the end of the study. Altogether, 13 individual bacteriocin types (out of 29 tested) were identified among investigated strains. Incidence of four E. coli genotypes was equally distributed in all groups of E. coli strains, with majority of genotype A (ranging from 81% to 88%). The following types of bacteriocins were most commonly revealed: colicins Ia/Ib (44%), microcin V (18%), colicin E1 (16%) and microcin H47 (6%). There was a difference in bacteriocinogeny between control group A (52/149, 35%) and groups with treatment at the end of the study: B: 31/122 (25%, P=0.120); C: 43/155 (28%, P=0.222); D: 29/134 (22%, P=0.020). There was a significantly lower prevalence of colicin Ib, microcins H47 and V (probiotics group, P<0.001), colicin E1 and microcin H47 (indomethacin group, P<0.001) and microcins H47 and V (probiotics and indomethacin group, P=0.025) compared to controls. Escherichia fergusonii (E. fergusonii) was identified in 6 animals (6/11 isolates from the rectum). One strain was non-colicinogenic, while all other strains of E. fergusonii solely produced colicin E1. All animals started and remained methanogenic despite the fact that EcN is a substantial hydrogen producer. There was an increase in breath methane (after the treatment) in 5/6 pigs from the indomethacin group (C). CONCLUSION: EcN did not exert long-term liveability in the porcine intestine. All experimental pigs remained methanogenic. Indomethacin and EcN administered together might produce the worst impact on bacteriocinogeny.

Klíčová slova
Bacteriocinogeny, Escherichia coli Nissle 1917, Experimental pigs, Indomethacin,
MeSH
antiflogistika nesteroidní škodlivé účinky farmakologie MeSH
bakteriociny metabolismus MeSH
dechové testy MeSH
Escherichia coli metabolismus MeSH
indomethacin škodlivé účinky farmakologie MeSH
lidé MeSH
metagenom MeSH
methan metabolismus MeSH
probiotika farmakologie MeSH
střevní sliznice účinky léků mikrobiologie MeSH
Sus scrofa MeSH
zvířata MeSH
Check Tag
lidé MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
antiflogistika nesteroidní MeSH
bakteriociny MeSH
indomethacin MeSH
methan MeSH

Článek

Indometacin v porodnictví: prínos a rizika
[Indomethacin in obstetrics: benefits and risks]

Kudela, M
Autor Kudela, M Gynek.-porod. klinika LF UP, Olomouc

Ceska gynekologie. 1997 Jun ; 62 (3) : 168-9.

Ceska Gynekol
ISSN 1210-7832
Zdroj

MeSH
indomethacin škodlivé účinky terapeutické užití MeSH
lidé MeSH
předčasná porodní činnost farmakoterapie MeSH
těhotenství MeSH
tokolytika škodlivé účinky terapeutické užití MeSH
Check Tag
lidé MeSH
těhotenství MeSH
ženské pohlaví MeSH
Publikační typ
časopisecké články MeSH
Názvy látek
indomethacin MeSH
tokolytika MeSH

Článek

Long-term effects of prenatal indomethacin administration on the pulmonary circulation in rats

The European respiratory journal. 1995 Feb ; 8 (2) : 209-15.

Eur Respir J
ISSN 0903-1936
Zdroj

Mechanical properties of the adult pulmonary vasculature are affected by perinatal experience of hypoxic pulmonary hypertension. In the present study, we followed the long-term effects of perinatal pulmonary hypertension induced by means other than hypoxia in rats. Daily injections of indomethacin (1 mg.kg-1 body weight (BW)) were given to the parturient rats. Their newborn pups had significantly increased number of muscularized peripheral pulmonary vessels. Pulmonary hypertension, however, did not persist to adulthood (mean pulmonary arterial pressure (Ppa) was 17.2 +/- 1.3 torr in the experimental group and 16.4 +/- 0.8 torr in controls). Pulmonary hypertension induced in adult rats by exposure to chronic hypoxia or by acute hypoxic challenges was similar in indomethacin-treated and control rats. Normoxic perfusion pressure/flow (P/Q) plots in isolated lungs were less steep in indomethacin-treated than in control rats. Acute hypoxia increased the slope of P/Q plots in indomethacin treated rats but not in controls. The described changes in the pulmonary vasculature induced by indomethacin are similar to those found previously in adult rats born in hypoxia. We conclude that perinatal pulmonary hypertension permanently modifies the pulmonary vasculature.

MeSH
arteria pulmonalis účinky léků embryologie MeSH
časové faktory MeSH
hypoxie komplikace MeSH
indomethacin škodlivé účinky MeSH
krysa rodu Rattus MeSH
plíce účinky léků patofyziologie MeSH
plicní hypertenze chemicky indukované patofyziologie MeSH
plicní oběh účinky léků MeSH
potkani Wistar MeSH
těhotenství MeSH
venae pulmonales účinky léků embryologie MeSH
zpožděný efekt prenatální expozice MeSH
zvířata MeSH
Check Tag
krysa rodu Rattus MeSH
mužské pohlaví MeSH
těhotenství MeSH
ženské pohlaví MeSH
zvířata MeSH
Publikační typ
časopisecké články MeSH
práce podpořená grantem MeSH
Názvy látek
indomethacin MeSH

Článek

Effect of sialoadenectomy on stomach lesions induced by indomethacin and ethanol in relation to gastric vascular permeability, the gastrin level and HCl secretion in rats

Physiological research. 1992 ; 41 (5) : 381-6.

Physiol Res
ISSN 0862-8408
Zdroj

Stomach lesions induced by indomethacin (20 mg.kg-1 i.p.) and ethanol (1 ml 95% intragastrically) were studied after a 24 hour fast in rats which had undergone sialoadenectomy. The size of the lesions was correlated with gastric HCl secretion, with gastric vascular permeability (determined from the Evans blue concentration in the stomach tissue after its i.v. administration) and with the serum gastrin level. These parameters were also studied in sialoadenectomized rats and in animals given epidermal growth factor (EGF) (50 lg.kg-1). It was found that sialoadenectomy significantly (p < 0.01) raised the incidence of stomach lesions after the administration of indomethacin and also after ethanol (p < 0.05). A significant increase in both basal and stimulated HCl secretion was found after sialoadenectomy. Both indomethacin and ethanol also increased gastric vascular permeability in rats not subjected to sialoadenectomy, but sialoadenectomy raised it significantly compared with the non-sialoadenectomized group. The serum gastrin levels fell after sialoadenectomy and the decrease was significant after the subsequent administration of indomethacin or ethanol. The administration of EGF to sialoadenectomized rats lowered the incidence of stomach lesions, inhibited HCl secretion and reduced vascular permeability. The lowered susceptibility of the gastric mucosa to the formation of lesions in sialoadenectomized rats given indomethacin or ethanol can be regarded as the outcome of the uptake of EGF.