The hepatic cytochrome p450 enzymes 1 A, 2A19 and 2E1 is very important for the elimination of skatole from the body of pigs. Impaired skatole metabolism, results in skatole accumulation, which give rise to off flavor of the meat. Several metabolites of skatole has been identified, however the role of these metabolites in the inhibition of the skatole metabolizing enzymes are not documented. Using microsomes from pigs and fish, we determined the ability of several skatole metabolites to inhibit CYP1 A, CYP2A19 and CYP2E1 dependent activity. Our results show that 2-aminoacetophenone is an inhibitor of porcine CYP2A19 and CYP2E1 activity, but not the piscine orthologues. In conclusion, there is species specific differences in the inhibition of CYP1 A and CYP2A19 dependent metabolism of probe substrates. This is relevant to the evaluation of different model systems and to the reduction of off flavor of meat.
- Klíčová slova
- Cytochrome P450, Pig, Rainbow trout, Skatole metabolism,
- MeSH
- acetofenony toxicita MeSH
- červené maso analýza MeSH
- cytochrom P-450 CYP1A1 antagonisté a inhibitory metabolismus MeSH
- cytochrom P-450 CYP2E1 metabolismus MeSH
- druhová specificita MeSH
- inhibitory cytochromu P450 CYP2E1 toxicita MeSH
- jaterní mikrozomy účinky léků metabolismus MeSH
- játra účinky léků metabolismus MeSH
- kumariny toxicita MeSH
- nitrofenoly toxicita MeSH
- oxaziny toxicita MeSH
- potrava z moře (živočišná) analýza MeSH
- prasata MeSH
- ryby MeSH
- skatol toxicita MeSH
- systém (enzymů) cytochromů P-450 metabolismus MeSH
- zvířata MeSH
- Check Tag
- mužské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 2-aminoacetophenone MeSH Prohlížeč
- 4-nitrophenol MeSH Prohlížeč
- acetofenony MeSH
- coumarin MeSH Prohlížeč
- cytochrom P-450 CYP1A1 MeSH
- cytochrom P-450 CYP2E1 MeSH
- ethoxyresorufin MeSH Prohlížeč
- inhibitory cytochromu P450 CYP2E1 MeSH
- kumariny MeSH
- nitrofenoly MeSH
- oxaziny MeSH
- skatol MeSH
- systém (enzymů) cytochromů P-450 MeSH
