OBJECTIVES: The aim of this study was to investigate the associations between urinary arsenic, oxidative stress, assessed by thiol/disulphide homeostasis, and lung diseases in firefighters. METHODS: The study conducted among the municipality-based male firefighters (n = 100) who were admitted to occupational diseases clinic for periodic medical examination. The control group consisted of non-exposed male office workers (n = 50). Urinary arsenic levels, thiol/disulphide homeostasis parameters of participants were determined. Also, lung diseases were assessed by chest X-ray and pulmonary function tests. RESULTS: The mean age and work year did not differ in the study and control group. The median urinary arsenic concentration of firefighters was significantly higher than in the control group: 15.65 (2.5-246) μg/L and 3 (0.10-6) μg/L, respectively (p < 0.001). The parameters of pulmonary function tests (PFT) FVC (%), FEV1 (%), FEV1/FVC ratio and FEF 25-75 (%) were all significantly lower in firefighters compared to controls. A significant increase in mean serum disulphide concentration (17.10 ± 8.31 μmol/L vs. 7.48 ± 5.91) (Fig. 1) and disulphide/native thiol % ratio: 3.63 (0.53-11.43) vs. 1.51 (0.03-7.65) (p < 0.001) were found between exposed group and controls. The Spearman's correlation analysis revealed a positive correlation between urinary arsenic and disulphide (r = 0.422, p < 0.001), disulphide/native thiol % ratio (r = 0.409, p < 0.001). Nevertheless, urinary arsenic correlated negatively with all PFT parameters including FVC (%), FEV1 (%), FEV1/FVC and FEF 25-75 (%) (p < 0.001). CONCLUSION: We showed the arsenic-induced oxidative stress in firefighters with impairments of several lung functions determined by thiol/disulphide homeostasis using a novel method.

• Klíčová slova
• X-ray abnormality, arsenic, firefighters, respiratory function disorder, thiol/disulphide homeostasis,
• MeSH
• arsen moč   MeSH
• biologické markery krev   moč   MeSH
• časná diagnóza MeSH
• disulfidy krev   MeSH
• dospělí MeSH
• hasiči * MeSH
• homeostáza MeSH
• lidé MeSH
• oxidační stres MeSH
• plicní nemoci krev   diagnóza   patofyziologie   MeSH
• rentgendiagnostika hrudníku MeSH
• respirační funkční testy MeSH
• sulfhydrylové sloučeniny krev   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Geografické názvy
• Turecko MeSH
• Názvy látek
• arsen MeSH
• biologické markery MeSH
• disulfidy MeSH
• sulfhydrylové sloučeniny MeSH

Sensitive assay method was developed for a parallel, rapid and precise determination of the most prominent oxidative stress biomarkers: 8-iso-prostaglandin F(2alpha), malondialdehyde and 4-hydroxynonenal. The method consisted of a pre-treatment part a solid-phase extraction, for rapid and effective isolation of biomarkers from body fluids (exhaled breath condensate, plasma and urine) and the detection method LC-ESI-MS/MS, where the selected reaction monitoring mode was used for its extremely high degree of selectivity and the stable-isotope-dilution assay for its high precision of quantification. The developed method was characterized by the following parameters: the imprecision was below 14.3%, the mean inaccuracy was determined to be lower than 13.1%. The method was tested on samples obtained from patients diagnosed with asbestosis, pleural hyalinosis or silicosis, i.e. occupational lung diseases caused by fibrogenic dusts, inducing oxidative stress in the respiratory system, and then compared to samples from healthy subjects. The difference in concentration levels of biomarkers between the two groups was perceptible in all the body fluids (the difference observed in an exhaled breath condensate was statistically most significant).

• MeSH
• aldehydy analýza   krev   moč   MeSH
• azbest toxicita   MeSH
• biologické markery analýza   krev   moč   MeSH
• dinoprost analogy a deriváty   analýza   krev   moč   MeSH
• extrakce na pevné fázi metody   MeSH
• lidé středního věku MeSH
• lidé MeSH
• malondialdehyd analýza   krev   moč   MeSH
• oxid křemičitý toxicita   MeSH
• oxidační stres * MeSH
• plicní nemoci krev   etiologie   metabolismus   moč   MeSH
• senioři MeSH
• studie případů a kontrol MeSH
• tandemová hmotnostní spektrometrie metody   MeSH
• tělesné tekutiny chemie   metabolismus   MeSH
• vysokoúčinná kapalinová chromatografie metody   MeSH
• Check Tag
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 4-hydroxy-2-nonenal MeSH Prohlížeč
• 8-epi-prostaglandin F2alpha MeSH Prohlížeč
• aldehydy MeSH
• azbest MeSH
• biologické markery MeSH
• dinoprost MeSH
• malondialdehyd MeSH
• oxid křemičitý MeSH

Alveolar macrophages (AM) originate from blood monocytes and, during the maturation process, undergo functional and morphological changes which are also reflected in their phenotypic pattern. Among the macrophage membrane antigens, adhesion molecules of the integrin family are particularly important for effector functions and cell-cell interactions. The aim of this study was to analyse the membrane expression of selected integrins by AM recovered from bronchoalveolar lavage (BAL) as compared to their precursors, peripheral blood monocytes (PBM). The cells were stained using a sensitive immunoperoxidase assay with 10 different monoclonal antibodies. The data showed a higher expression by AM than PBM of all but one of the studied adhesion molecules. The only exception was CD11b (Mac-1, CR3) which showed a higher expression in PBM than in AM. Several molecules, for example, CD49d (VLA-4), CD51 (vitronectin receptor), and CD54 (intercellular adhesion molecule-1, ICAM-1) were found to be upregulated by AM in patients with a lymphocytic pattern of BAL. In contrast, the phenotype of PBM does not show any changes in these patients. In conclusion, we have demonstrated differences in the expression of integrins between AM and PBM which can be partially responsible for some of their functional differences.

• MeSH
• alveolární makrofágy imunologie   MeSH
• bronchoalveolární lavážní tekutina imunologie   MeSH
• buněčná membrána imunologie   MeSH
• CD antigeny analýza   MeSH
• dospělí MeSH
• imunohistochemie MeSH
• integriny analýza   MeSH
• lidé středního věku MeSH
• lidé MeSH
• monocyty imunologie   MeSH
• neparametrická statistika MeSH
• plicní nemoci krev   imunologie   MeSH
• separace buněk MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Názvy látek
• CD antigeny MeSH
• integriny MeSH

Serum concentrations of tissue polypeptide antigen (TPA) were examined by an immunoradiometric technique in 114 patients with bronchogenic carcinoma and in 55 patients with noncancerous lung diseases. The sensitivity of TPA examination in bronchogenic carcinoma was 67.5% and was increased in advanced stages of the disease. No statistically significant differences were observed between histologic types of bronchogenic carcinoma. In nonmalignant lung diseases, elevated levels of TPA were observed in 21.8% of patients. TPA is of little value in the diagnosis of bronchogenic carcinoma; however, it may be useful as an auxiliary criterion for staging.

• MeSH
• adenokarcinom krev   patologie   MeSH
• bronchogenní karcinom krev   patologie   MeSH
• dospělí MeSH
• imunoradiometrická analýza metody   MeSH
• karcinom krev   patologie   MeSH
• lidé středního věku MeSH
• lidé MeSH
• malobuněčný karcinom krev   patologie   MeSH
• nádorové biomarkery krev   MeSH
• nádory plic krev   patologie   MeSH
• peptidy krev   MeSH
• plicní nemoci krev   MeSH
• referenční hodnoty MeSH
• senioři MeSH
• spinocelulární karcinom krev   patologie   MeSH
• staging nádorů MeSH
• tkáňový polypeptidový antigen MeSH
• velkobuněčný karcinom krev   patologie   MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• mužské pohlaví MeSH
• senioři MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• srovnávací studie MeSH
• Názvy látek
• nádorové biomarkery MeSH
• peptidy MeSH
• tkáňový polypeptidový antigen MeSH

A set of 620 patients was examined. Out of them, 245 suffered from lung carcinoma of different type and stage, 28 suffered from other malignant tumors, 37 were affected with benign tumors, and 166 were suffering from a nonmalignant respiratory disease (tuberculosis, nonspecific pneumonia, chronic bronchitis, abscesses, cysts, asthma, lung fibrosis, bronchiectasis and sarcoidosis). In addition to these patients, 144 blood donors were examined who represented the control group of healthy individuals. In a blind test another set of 266 persons was examined. By completing the values of selected markers (orosomucoid, prealbumin, glycoprotein electrophoresis, erythrocyte sedimentation, age of the individual, and the number of smoked cigarettes) into the discrimination rule and by calculating the discrimination function, a sensitivity of 80.6% and a specificity of 75.6% were obtained. A comparative cytological examination of the same set revealed lower sensitivity (61.0%) but higher specificity (98.0%). These values were verified in a blind test, as the patients were admitted to the hospital. Sensitivity in lung cancer was found to be 83.9%; in nonmalignant diseases the respective value was 77.1%. This approach can be applied to individuals suspect of cancer, in secondary prevention and in individuals with a high risk of lung cancer.

• MeSH
• chybná diagnóza MeSH
• diferenciální diagnóza MeSH
• dospělí MeSH
• kouření MeSH
• lidé středního věku MeSH
• lidé MeSH
• nádorové biomarkery krev   MeSH
• nádory plic krev   diagnóza   prevence a kontrola   MeSH
• plicní nemoci krev   diagnóza   MeSH
• prediktivní hodnota testů MeSH
• rizikové faktory MeSH
• senioři MeSH
• statistika jako téma * MeSH
• věkové faktory MeSH
• Check Tag
• dospělí MeSH
• lidé středního věku MeSH
• lidé MeSH
• senioři MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• nádorové biomarkery MeSH

• MeSH
• alfa-1-antitrypsin krev   MeSH
• chronická nemoc MeSH
• deficit alfa1-antitrypsinu MeSH
• dítě MeSH
• lidé MeSH
• plicní nemoci krev   enzymologie   MeSH
• Check Tag
• dítě MeSH
• lidé MeSH
• Publikační typ
• anglický abstrakt MeSH
• časopisecké články MeSH
• Názvy látek
• alfa-1-antitrypsin MeSH

• Klíčová slova
• CARCINOMA, BRONCHOGENIC/blood *, ESTERASES/blood *, LUNG DISEASES/blood *,
• MeSH
• bronchogenní karcinom krev   MeSH
• esterasy krev   MeSH
• karcinom * MeSH
• lidé MeSH
• plicní nemoci krev   MeSH
• sérum * MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• esterasy MeSH