Extensive exploitation of titanium dioxide nanoparticles (TiO2NPs) augments rapid release into the marine environment. When in contact with the body fluids of marine invertebrates, TiO2NPs undergo a transformation and adhere various organic molecules that shape a complex protein corona prior to contacting cells and tissues. To elucidate the potential extracellular signals that may be involved in the particle recognition by immune cells of the sea urchin Paracentrotus lividus, we investigated the behavior of TiO2NPs in contact with extracellular proteins in vitro. Our findings indicate that TiO2NPs are able to interact with sea urchin proteins in both cell-free and cell-conditioned media. The two-dimensional proteome analysis of the protein corona bound to TiO2NP revealed that negatively charged proteins bound preferentially to the particles. The main constituents shaping the sea urchin cell-conditioned TiO2NP protein corona were proteins involved in cellular adhesion (Pl-toposome, Pl-galectin-8, Pl-nectin) and cytoskeletal organization (actin and tubulin). Immune cells (phagocytes) aggregated TiO2NPs on the outer cell surface and within well-organized vesicles without eliciting harmful effects on the biological activities of the cells. Cells showed an active metabolism, no oxidative stress or caspase activation. These results provide a new level of understanding of the extracellular proteins involved in the immune-TiO2NP recognition and interaction in vitro, confirming that primary immune cell cultures from P. lividus can be an optional model for swift and efficient immune-toxicological investigations.
- Klíčová slova
- biocorona, echinoderm, extracellular signaling, immune-adhesome, in vitro-ex vivo model, proxy to human,
- MeSH
- buněčná adheze imunologie MeSH
- fagocyty imunologie MeSH
- galektiny imunologie MeSH
- glykoproteiny imunologie MeSH
- ježovky imunologie MeSH
- nanočástice aplikace a dávkování MeSH
- nektiny imunologie MeSH
- Paracentrotus imunologie MeSH
- proteinová korona imunologie MeSH
- proteom imunologie MeSH
- titan imunologie MeSH
- zvířata MeSH
- Check Tag
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- galektiny MeSH
- glykoproteiny MeSH
- nektiny MeSH
- proteinová korona MeSH
- proteom MeSH
- titan MeSH
- titanium dioxide MeSH Prohlížeč
- toposome glycoprotein complex MeSH Prohlížeč
Titanium dioxide nanofibres (TiO2) were intratracheally instilled in dose of 4 mg/0.2 mL saline solution per animal (Wistar rats). After 48 hours and 14 days the animals were exsanguinated (under i.p. thiopental narcosis), bronchoalveolar lavage (BAL) was perfomed and cells from BAL fluid were isolated. Following inflammatory, cytotoxic and oxidative stress BAL parameters were examined: differential cell count (% of alveolar macrophages (AM), polymorphonuclears and lymphocytes); the viability and phagocytic activity of AM; the proportion of immature cells; the proportion of multinucleated cells; count of AM/mL lavage; count of BAL cells/mL lavage; the level of ascorbic acid and activity of superoxide dismutase, both in tissue homogenate and in bronchoalveolar lavage fluid. The majority of examined BAL parameters in the acute and subacute phase in our study suggest serious inflammatory and cytotoxic processes in lung after exposure to TiO2.
- MeSH
- alveolární makrofágy imunologie MeSH
- bronchoalveolární lavážní tekutina cytologie MeSH
- krysa rodu Rattus MeSH
- leukocyty imunologie MeSH
- mediátory zánětu imunologie MeSH
- nanovlákna aplikace a dávkování škodlivé účinky MeSH
- oxidační stres imunologie MeSH
- potkani Wistar MeSH
- titan aplikace a dávkování škodlivé účinky imunologie MeSH
- zvířata MeSH
- Check Tag
- krysa rodu Rattus MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- mediátory zánětu MeSH
- titan MeSH
- titanium dioxide MeSH Prohlížeč
OBJECTIVES: In most of patients in need of implantation treatment in the oral cavity, implants heal well, nevertheless, there are some individuals, in whom titanium implants fail for reasons, which remain unclear. DESIGN: The aim of our study was to determine if there is a difference between metal influenced IL-1β, IL-4, IL-6, TNF-α and IFN-γ cytokines production in patients with successfully healed implants compared to those, whose implant therapy was unsuccessful. SETTING: The two study groups included 12 patients with failed dental titanium implants and 9 patients with successfully healed implants. In the subjects, cytokine production was established after lymphocyte cultivation with mercury, nickel and titanium antigens. RESULTS: IL-1β levels were significantly increased in all patients after stimulation with titanium and in patients with accepted implants compared to patients with failed implants after the stimulation with mercury and titanium. Titanium caused significantly increased IL-6 production in all patients. TNF-α and IFN-γ levels were also significantly increased after the stimulation with titanium. Significantly increased TNF-α levels were found in patients with accepted implants as compared to patients with failed implants. CONCLUSIONS: Increased production of IL-1β a IL-6 cytokines in reaction to titanium and increased production of TNF-α and IFN-γ cytokines in reaction to mercury, which is very often present in the form of amalgam in the oral cavity of persons in need of implant therapy, can play an important role in immune reactions during implant healing process. In patients with failed titanium implants, decreased production of these cytokines may participate in implant failure.
- MeSH
- cytokiny metabolismus MeSH
- dospělí MeSH
- implantace zubů přístrojové vybavení MeSH
- interferon gama metabolismus MeSH
- interleukin-1beta metabolismus MeSH
- interleukin-4 metabolismus MeSH
- interleukin-6 metabolismus MeSH
- kovy imunologie MeSH
- kultivované buňky MeSH
- lidé středního věku MeSH
- lidé MeSH
- lymfocyty imunologie MeSH
- neúspěšná terapie MeSH
- nikl imunologie MeSH
- rtuť imunologie MeSH
- titan imunologie MeSH
- TNF-alfa metabolismus MeSH
- výsledek terapie MeSH
- zubní implantáty * MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- srovnávací studie MeSH
- Názvy látek
- cytokiny MeSH
- interferon gama MeSH
- interleukin-1beta MeSH
- interleukin-4 MeSH
- interleukin-6 MeSH
- kovy MeSH
- nikl MeSH
- rtuť MeSH
- titan MeSH
- TNF-alfa MeSH
- zubní implantáty * MeSH
