OBJECTIVE: The objective of the study is to examine the role of microscopy using stained smears for diagnosis of vulvovavaginal infections: DESIGN: Description of different scoring systems. SETTINGS: Department of Gynecology and Obstetrics, First Medical Faculty, Charles University; General Teaching Hospital, Prague; Institute of Clinical Chemistry and Laboratory Medicine; Clinical Microbiology and Antibiotic Center, First Medical Faculty, Charles University. MATERIAL AND METHODS: Presentation of our practical skills in microscopic diagnoses of vulvovaginal infections. CONCLUSIONS: Vulvovaginal infections are a common problem which we encounter in daily gynaecological practice. Microscopic examination represents the gold standard in the diagnosis of vulvovaginal infections. However, providing microscopy in an outpatient setting is very time-consuming. The vaginal smear can be sent to a laboratory to stain and to be microscopically examined under oil immersion. For this purpose we recommend taking two smears for Gram and Giemsa stain and combining microscopical examination with cultures for detecting the presence of Candida species and for Trichomonas vaginalis. Where appropriate, it is also necessary to obtain cervical smears for detection of C. trachomatis and N. gonnorhoeae infection.
- MeSH
- barvicí látky * MeSH
- kandidóza vulvovaginální diagnóza mikrobiologie MeSH
- lidé MeSH
- trichomonádová vaginitida diagnóza mikrobiologie MeSH
- vaginální stěr * MeSH
- vulvovaginitida diagnóza mikrobiologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- barvicí látky * MeSH
Vulvovaginal discomfort is generally the most common reason for visiting a gynaecological clinic. The acute complaints are, in most cases, solved by a gynaecologist himself and the decision procedure is based on possibilities arising from outpatient examination. If need be, the evaluation of wet preparation should be carried out. In many cases we have been confronted with discrepant diagnosis where severe difficulties are not accompanied by relevant development of inflammatory changes and thus the diagnosis gives us an impression of quandary. In this case microbiological examination is the logic solution. With regard to changing living conditions we can expect in the future, that clinical symptoms of vulvovaginitis will not be entirely clear. The overview article points out the possibilities, which a gynecologist is able to use within differential diagnosis in the outpatient department. It also emphasis the necessity of interdisciplinary co-operation, particularly in patients with chronic difficulties where erudite evaluation of fixed preparation and culture examination are beneficial.
- MeSH
- lidé MeSH
- vulvovaginitida * diagnóza mikrobiologie terapie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- přehledy MeSH
OBJECTIVE: To evaluate the occurrence of mixed and unclassifiable vulvovaginitis (i.e. those, which fulfill the diagnostic criteria of several diagnostic units or no diagnostic unit) in symptomatic and asymptomatic women. TYPE OF STUDY: Prospective study. METHODS: In 412 women (115 of them asymptomatic) the authors established the diagnosis of vulvovaginitis on the basis of gynecological examination, pH, the amine test and microscopic examination according to Giemsa and Gram. RESULTS: Mycosis was diagnosed in 15.5% women (in 9,6% of asymptomatic ones), lactobacillosis in u 5.6% (in 7.0% of asymptomatic), anaerobic vaginosis in 10.7% (8.7% of asymptomatic), aerobic vaginitis in 7.7% women (4.3% of asymptomatic). U 15.0% mixed infections were diagnosed (in 61% asymptomatic). U 29.4% symptomatic women the diagnostic criteria were not fulfilled for any nosological unit. CONCLUSION: Vulvovaginal mycosis, lactobacillosis, anaerobic vaginosis, aerobic vaginosis were considered as dysmicrobia conditions. The authors demonstrated a high occurrence of more units ("clear" diagnoses to "mixed" diagnoses being in the ratio of 1.62:1). The authors also demonstrated a high occurrence of mixed infections in asymptomatic women (36.0%). On the contrary, in 29.4% of symptomatic women the diagnosis could not be established, the findings being "normal" or "unclassifiable".
- MeSH
- bakteriální vaginóza diagnóza mikrobiologie MeSH
- lidé MeSH
- mykózy diagnóza mikrobiologie MeSH
- vulvovaginitida diagnóza mikrobiologie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
AIM OF THE STUDY: The evaluation of combined and miscellaneous vulvovaginal infections incidence and their treatment with combined vaginal products containing nifuratel and nystatin. DESIGN: Prospective study. SETTING: Gynecologic outpatient department LEVRET, Prague; Laboratories of Microbiology AescuLab, Prague. METHODS: 70 consecutive patients were examined with complaint of vaginal fluor and/or pruritus. We established macroscopic features of fluor, pH, amine test and mounts stained with Giemsa and Gram. We qualified the cases with more diagnostic criteria (mycosis, lactobacillosis, anaerobic vaginosis, aerobic vaginitis) as combined infection, those with no diagnostic criteria as miscellaneous. We treated all patients with vaginal tablets nystatin + nifuratel (Macmiror complex). We prescribed clotrimazol cream, if pruritus was present. We evaluated withdrawals of symptoms and relapses during 3 months after treatment. RESULTS: Combined infection was found in 21 patients from 70 (30%). The most frequent combination was that of mycosis and aerobic vaginitis (13/70, 18.6%) or mycosis and anaerobic vaginosis (4/70, 5.7%); 11 patients fulfilled criteria of no diagnosis. We concluded them as "miscelaneous". The treatment was successful in all cases, 10 women relapsed in 3 months. CONCLUSIONS: Combined vaginal infection findings are present very often (30%), likewise miscellaneous ones (15%) occur. The treatment of these women in successful with vaginal tablets with nystatin + nifuratel.
- MeSH
- antibakteriální látky aplikace a dávkování MeSH
- antifungální látky aplikace a dávkování MeSH
- antitrichomonádové látky aplikace a dávkování MeSH
- aplikace intravaginální MeSH
- bakteriální vaginóza komplikace farmakoterapie MeSH
- dospělí MeSH
- fixní kombinace léků MeSH
- kandidóza vulvovaginální komplikace farmakoterapie MeSH
- klotrimazol aplikace a dávkování MeSH
- lidé středního věku MeSH
- lidé MeSH
- nifuratel MeSH
- nystatin aplikace a dávkování MeSH
- trichomonádová vaginitida komplikace farmakoterapie MeSH
- vulvovaginitida farmakoterapie mikrobiologie MeSH
- Check Tag
- dospělí MeSH
- lidé středního věku MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- klinické zkoušky MeSH
- Názvy látek
- antibakteriální látky MeSH
- antifungální látky MeSH
- antitrichomonádové látky MeSH
- fixní kombinace léků MeSH
- klotrimazol MeSH
- nifuratel MeSH
- nystatin MeSH
- MeSH
- lidé MeSH
- vulvovaginitida diagnóza mikrobiologie terapie MeSH
- Check Tag
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- dopisy MeSH
- MeSH
- antibakteriální látky aplikace a dávkování MeSH
- antifungální látky aplikace a dávkování MeSH
- antitrichomonádové látky aplikace a dávkování MeSH
- dítě MeSH
- kombinovaná farmakoterapie MeSH
- lidé MeSH
- mladiství MeSH
- nifuratel aplikace a dávkování MeSH
- nystatin aplikace a dávkování MeSH
- vulvovaginitida farmakoterapie mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- antibakteriální látky MeSH
- antifungální látky MeSH
- antitrichomonádové látky MeSH
- nifuratel MeSH
- nystatin MeSH
- MeSH
- bakteriální infekce mikrobiologie MeSH
- dítě MeSH
- dospělí MeSH
- Gardnerella vaginalis izolace a purifikace MeSH
- Haemophilus izolace a purifikace MeSH
- hemofilové infekce mikrobiologie MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- syndrom MeSH
- vagina mikrobiologie MeSH
- vulvovaginitida mikrobiologie MeSH
- Check Tag
- dítě MeSH
- dospělí MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- MeSH
- bacilární dyzentérie komplikace mikrobiologie MeSH
- dítě MeSH
- lidé MeSH
- Shigella sonnei izolace a purifikace MeSH
- vulvovaginitida etiologie mikrobiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- kazuistiky MeSH
- MeSH
- buněčné linie MeSH
- epitel * MeSH
- epitelové buňky * MeSH
- infekční rinotracheitida skotu MeSH
- kultivace virů MeSH
- ledviny patologie MeSH
- skot MeSH
- vulvovaginitida mikrobiologie veterinární MeSH
- zvířata MeSH
- Check Tag
- skot MeSH
- ženské pohlaví MeSH
- zvířata MeSH
- Publikační typ
- časopisecké články MeSH