Periodontitis and dental caries are two major bacterially induced, non-communicable diseases that cause the deterioration of oral health, with implications in patients' general health. Early, precise diagnosis and personalized monitoring are essential for the efficient prevention and management of these diseases. Here, we present a disk-shaped microfluidic platform (OralDisk) compatible with chair-side use that enables analysis of non-invasively collected whole saliva samples and molecular-based detection of ten bacteria: seven periodontitis-associated (Aggregatibacter actinomycetemcomitans, Campylobacter rectus, Fusobacterium nucleatum, Prevotella intermedia, Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola) and three caries-associated (oral Lactobacilli, Streptococcus mutans, Streptococcus sobrinus). Each OralDisk test required 400 µL of homogenized whole saliva. The automated workflow included bacterial DNA extraction, purification and hydrolysis probe real-time PCR detection of the target pathogens. All reagents were pre-stored within the disk and sample-to-answer processing took < 3 h using a compact, customized processing device. A technical feasibility study (25 OralDisks) was conducted using samples from healthy, periodontitis and caries patients. The comparison of the OralDisk with a lab-based reference method revealed a ~90% agreement amongst targets detected as positive and negative. This shows the OralDisk's potential and suitability for inclusion in larger prospective implementation studies in dental care settings.
- Klíčová slova
- caries, dental practice, oral health, periodontitis, point-of-care diagnostics, saliva diagnostics, treatment monitoring,
- MeSH
- lidé MeSH
- mikrofluidní analytické techniky * MeSH
- orální zdraví * MeSH
- parodontitida * diagnóza MeSH
- sliny mikrobiologie MeSH
- zubní kaz * diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
Global healthcare systems are struggling with the enormous burden associated with infectious diseases, as well as the incessant rise of antimicrobial resistance. In order to adequately address these issues, there is an urgent need for rapid and accurate infectious disease diagnostics. The H2020 project DIAGORAS aims at diagnosing oral and respiratory tract infections using a fully integrated, automated and user-friendly platform for physicians' offices, schools, elderly care units, community settings, etc. Oral diseases (periodontitis, dental caries) will be detected via multiplexed, quantitative analysis of salivary markers (bacterial DNA and host response proteins) for early prevention and personalised monitoring. Respiratory Tract Infections will be diagnosed by means of DNA/RNA differentiation so as to identify their bacterial or viral nature. Together with antibiotic resistance screening on the same platform, a more efficient treatment management is expected at the point-of-care. At the heart of DIAGORAS lies a centrifugal microfluidic platform (LabDisk and associated processing device) integrating all components and assays for a fully automated analysis. The project involves an interface with a clinical algorithm for the comprehensive presentation of results to end-users, thereby increasing the platform's clinical utility. DIAGORAS' performance will be validated at clinical settings and compared with gold standards.
- MeSH
- bakteriální léková rezistence * MeSH
- centrifugace metody MeSH
- DNA bakterií analýza MeSH
- individualizovaná medicína metody MeSH
- infekce dýchací soustavy diagnóza mikrobiologie virologie MeSH
- laboratorní automatizace MeSH
- lidé MeSH
- mikrofluidní analytické techniky MeSH
- parodontitida diagnóza mikrobiologie MeSH
- RNA virová analýza MeSH
- sliny imunologie mikrobiologie MeSH
- zubní kaz diagnóza MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- DNA bakterií MeSH
- RNA virová MeSH
AIM: To evaluate the association between anthropometric measurements and dental caries in school children. METHODS: The study was conducted on 245 primary school children (50.2% boys, 49.8% girls,), aged 5 to 9 years. The prevalence and severity of dental caries was measured using the decayed, missing or filled surfaces (dmfs, DMFS) and teeth (dmft, DMFT) indices. RESULTS: Mean dmft indices in children for boys and girls were 5.5 +/- 3.92 and 5.0 +/- 3.64, respectively. The prevalence of children with dental caries (dmft > or = 1) was 84.9%. The prevalence of children with body mass index (BMI) <-1SD and > or = +1SD was 15.9% and 22.9%, respectively. Dental caries were found in 89.7% of children with low body weight (including underweight and at risk for underweight) and in 66.1% of overweight-obese children (p<0.05). Similarly, high indices were detected significantly more often in children with low body weight (p<0.05). Height, weight, BMI and percent of fat mass were found to be negatively correlated with dmft indices (r:-0.141, p: 0.028; r:-0.171, p: 0.007; r:-0.139, p: 0.030; r:-0.158, p: 0.013, respectively). CONCLUSION: Children with low body weight have a higher risk of developing dental caries than overweight-obese children. For these reasons, the evaluation of nutritional status in children should be implemented in control programs for dental caries both on the community and individual levels.
- MeSH
- antropometrie MeSH
- dítě MeSH
- DMF Index MeSH
- hubenost komplikace epidemiologie MeSH
- index tělesné hmotnosti MeSH
- lidé MeSH
- nadváha komplikace epidemiologie MeSH
- předškolní dítě MeSH
- průřezové studie MeSH
- průzkumy a dotazníky MeSH
- rizikové faktory MeSH
- školy MeSH
- zubní kaz komplikace diagnóza epidemiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mužské pohlaví MeSH
- předškolní dítě MeSH
- ženské pohlaví MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Geografické názvy
- Turecko epidemiologie MeSH
- MeSH
- dietní sacharidy aplikace a dávkování MeSH
- dítě MeSH
- DMF Index MeSH
- lidé MeSH
- mladiství MeSH
- obezita patofyziologie MeSH
- sexuální faktory MeSH
- tělesná hmotnost MeSH
- zubní kaz diagnóza epidemiologie MeSH
- Check Tag
- dítě MeSH
- lidé MeSH
- mladiství MeSH
- mužské pohlaví MeSH
- ženské pohlaví MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- Názvy látek
- dietní sacharidy MeSH
- MeSH
- dítě MeSH
- fenylketonurie komplikace MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- věkové faktory MeSH
- zubní kaz diagnóza MeSH
- Check Tag
- dítě MeSH
- kojenec MeSH
- lidé MeSH
- mladiství MeSH
- předškolní dítě MeSH
- Publikační typ
- anglický abstrakt MeSH
- časopisecké články MeSH
- MeSH
- lidé MeSH
- zubní kaz diagnóza epidemiologie etiologie patologie MeSH
- zubní sklovina patologie MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Geografické názvy
- Československo MeSH
