Two DNA-based techniques were used for species identification of enterococci. PvuII digestion of the genus-specific PCR product yielded four different restriction profiles among 20 enterococcal species; one of them was species-specific for E. faecium. In the second case, 32 reference strains belonging to 20 enterococcal species were divided to 12 groups by amplification of internal transcribed spacer of rRNA operon. Interspecies and some intraspecies profile variability was determined. Both methods gave similar results.

• MeSH
• Species Specificity MeSH
• Enterococcus classification   genetics   metabolism   MeSH
• DNA, Intergenic chemistry   genetics   MeSH
• Polymerase Chain Reaction methods   MeSH
• DNA, Protozoan chemistry   genetics   MeSH
• Deoxyribonucleases, Type II Site-Specific metabolism   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• CAGCTG-specific type II deoxyribonucleases MeSH Browser
• DNA, Intergenic MeSH
• DNA, Protozoan MeSH
• Deoxyribonucleases, Type II Site-Specific MeSH