In the present work we isolated and identified various indigenous Saccharomyces cerevisiae strains and screened them for the selected oenological properties. These S. cerevisiae strains were isolated from berries and spontaneously fermented musts. The grape berries (Sauvignon blanc and Pinot noir) were grown under the integrated and organic mode of farming in the South Moravia (Czech Republic) wine region. Modern genotyping techniques such as PCR-fingerprinting and interdelta PCR typing were employed to differentiate among indigenous S. cerevisiae strains. This combination of the methods provides a rapid and relatively simple approach for identification of yeast of S. cerevisiae at strain level. In total, 120 isolates were identified and grouped by molecular approaches and 45 of the representative strains were tested for selected important oenological properties including ethanol, sulfur dioxide and osmotic stress tolerance, intensity of flocculation and desirable enzymatic activities. Their ability to produce and utilize acetic/malic acid was examined as well; in addition, H2S production as an undesirable property was screened. The oenological characteristics of indigenous isolates were compared to a commercially available S. cerevisiae BS6 strain, which is commonly used as the starter culture. Finally, some indigenous strains coming from organically treated grape berries were chosen for their promising oenological properties and these strains will be used as the starter culture, because application of a selected indigenous S. cerevisiae strain can enhance the regional character of the wines.

• Klíčová slova
• ITS-PCR-RFLP, Interdelta PCR typing, Oenological properties, Saccharomyces genus, Species-specific primers,
• MeSH
• bakteriální adheze MeSH
• DNA fingerprinting MeSH
• ethanol toxicita   MeSH
• fyziologický stres MeSH
• kyselina octová metabolismus   MeSH
• maláty metabolismus   MeSH
• molekulární typizace MeSH
• mykologické určovací techniky MeSH
• osmotický tlak MeSH
• oxid siřičitý toxicita   MeSH
• polymerázová řetězová reakce MeSH
• Saccharomyces cerevisiae klasifikace   genetika   izolace a purifikace   fyziologie   MeSH
• sulfan metabolismus   MeSH
• tolerance léku MeSH
• Vitis mikrobiologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• srovnávací studie MeSH
• Geografické názvy
• Česká republika MeSH
• Názvy látek
• ethanol MeSH
• kyselina octová MeSH
• maláty MeSH
• malic acid MeSH Prohlížeč
• oxid siřičitý MeSH
• sulfan MeSH