The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates of Pleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.

• MeSH
• aktivace enzymů účinky léků   MeSH
• azosloučeniny metabolismus   MeSH
• lakasa metabolismus   MeSH
• látky znečišťující životní prostředí metabolismus   MeSH
• měď farmakologie   MeSH
• peroxidasy metabolismus   MeSH
• Pleurotus klasifikace   enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• azosloučeniny MeSH
• lakasa MeSH
• látky znečišťující životní prostředí MeSH
• manganese peroxidase MeSH Prohlížeč
• měď MeSH
• Orange G MeSH Prohlížeč
• peroxidasy MeSH

The potential use of fungal pellets for decolorization of the textile dyeing wastewater was evaluated. The live pellets of the fungus Phanerochaete chrysosporium were found to remove more than 95% of the color of this wastewater within 1 d. The dye-removal capacity was a function of time and was proportional to the agitation rate; the optimum temperature was 30 degrees C. Both live and dead pellets were further examined in a repeated-batch mode for 5 d. The decolorization performance of live pellets remained high and stable for 5 d and they showed twice to thrice higher decolorization capacity than dead pellets.

• MeSH
• biodegradace MeSH
• chemické látky znečišťující vodu metabolismus   MeSH
• indoly metabolismus   MeSH
• Phanerochaete metabolismus   MeSH
• textilní průmysl metody   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• chemické látky znečišťující vodu MeSH
• indirubin MeSH Prohlížeč
• indoly MeSH

Fluorescein derivatives added into the growth medium were decolorized during submerged cultivation of Phanerochaete chrysosporium. The highest decrease of absorbance A450 was observed in the growth phase regardless of the presence of inducers Tween 80 or poly(ethylene glycol) (PEG). Fluorescein linked to PEG was prepared and, after addition to cultures, shown to stimulate the production of lignin peroxidase. Passing of fluorescing substances into hyphae (observed by confocal microscopy) showed that they were concentrated on some structures inside hyphae.

• MeSH
• enzymová indukce MeSH
• fluorescein chemie   metabolismus   MeSH
• fluorescenční barviva chemie   metabolismus   MeSH
• hyfy metabolismus   MeSH
• kultivační média MeSH
• peroxidasy metabolismus   MeSH
• Phanerochaete růst a vývoj   metabolismus   MeSH
• polyethylenglykoly chemie   farmakologie   MeSH
• polysorbáty farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• fluorescein MeSH
• fluorescenční barviva MeSH
• kultivační média MeSH
• lignin peroxidase MeSH Prohlížeč
• peroxidasy MeSH
• polyethylenglykoly MeSH
• polysorbáty MeSH

Molasses wastewater (vinasse; the by-product of distillation of fermented sugar) was decolorized and its chemical oxygen demand (COD) was reduced in static cultivation using the fungi Coriolus versicolor, Funalia trogii, Phanerochaete chrysosporium and Pleurotus pulmonarius ('Pleurotus sajorcaju'). The effect of cotton stalk on decolorizing and COD removing capability of four fungi was determined. In the entire concentration range tested (10-30%), wastewater was effectively decolorized by C. versicolor and F. trogii. Cotton stalk addition stimulated the decolorization activity of all fungi. The utilization of cotton stalk represents several advantages due to its function as an attachment place and as a source of nutrients; its use also reduces process costs.

• MeSH
• barva MeSH
• biodegradace * MeSH
• fermentace MeSH
• houby metabolismus   MeSH
• melasa * MeSH
• mikrobiologie vody MeSH
• odpad tekutý - odstraňování MeSH
• Pleurotus metabolismus   MeSH
• Polyporales metabolismus   MeSH
• průmyslový odpad * MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• průmyslový odpad * MeSH

The production of manganese-dependent peroxidase (MnP) by Phanerochaete chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 mukat/L were obtained, respectively. The in vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).

• MeSH
• barva MeSH
• barvicí látky metabolismus   MeSH
• biodegradace MeSH
• bioreaktory * MeSH
• dřevo MeSH
• kultivační média MeSH
• peroxidasy biosyntéza   metabolismus   MeSH
• Phanerochaete enzymologie   růst a vývoj   MeSH
• spektrofotometrie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• barvicí látky MeSH
• kultivační média MeSH
• lignin peroxidase MeSH Prohlížeč
• manganese peroxidase MeSH Prohlížeč
• peroxidasy MeSH

The effect of paraquat on the activities of antioxidant defense and detoxifying enzymes of the white-rot fungus Funalia trogii was determined. Paraquat increased the activities of glutathione reductase (GR), glutathione transferase (GT) and superoxide dismutase at 1 mmol/L, while at 0.1 mmol/L it did not affect the activity of GR and GT. It depressed the catalase activity and the amount of glutathione at all concentrations used. Paraquat treatment probably depresses antioxidant defense components such as catalase and glutathione.

• MeSH
• antioxidancia metabolismus   MeSH
• glutathion metabolismus   MeSH
• glutathionreduktasa metabolismus   MeSH
• glutathiontransferasa metabolismus   MeSH
• herbicidy toxicita   MeSH
• katalasa metabolismus   MeSH
• kinetika MeSH
• paraquat toxicita   MeSH
• Polyporales účinky léků   enzymologie   metabolismus   MeSH
• superoxiddismutasa metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• antioxidancia MeSH
• glutathion MeSH
• glutathionreduktasa MeSH
• glutathiontransferasa MeSH
• herbicidy MeSH
• katalasa MeSH
• paraquat MeSH
• superoxiddismutasa MeSH

The ability to decolorize four synthetic dyes (Phenol Red, Evans Blue, Eosin Yellowish and Poly B411) in five Pleurotus ostreatus strains (a parental strain and four isolates derived from it) was determined. Two of the isolates had markedly higher and other two substantially lower production of ligninolytic enzymes and hydrogen peroxide than the parental strain. Like the parental strain, the higher-producing isolates were able to decolorize all the tested dyes, but not to a higher extent than the parental strain. In contrast, two lower-producing isolates exhibited slow decolorization, which was incomplete even at the end of cultivation. Evans Blue and Eosin Yellowish strongly suppressed the growth of the strains, while Phenol Red and Poly B411 induced none or only a very slight growth reduction.

• MeSH
• barva MeSH
• barvicí látky metabolismus   MeSH
• lakasa MeSH
• oxidoreduktasy metabolismus   MeSH
• peroxid vodíku metabolismus   MeSH
• peroxidasy metabolismus   MeSH
• Pleurotus chemie   enzymologie   metabolismus   MeSH
• textilní průmysl MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• barvicí látky MeSH
• lakasa MeSH
• manganese peroxidase MeSH Prohlížeč
• oxidoreduktasy MeSH
• peroxid vodíku MeSH
• peroxidasy MeSH

Neem hull waste (containing a high amount of lignin and other phenolic compounds) was used for lignin peroxidase production by Phanerochaete chrysosporum under solid-state fermentation conditions. Maximum decolorization achieved by partially purified lignin peroxidase was 80% for Porocion Brilliant Blue HGR, 83 for Ranocid Fast Blue, 70 for Acid Red 119 and 61 for Navidol Fast Black MSRL. The effects of different concentrations of veratryl alcohol, hydrogen peroxide, enzyme and dye on the efficiency of decolorization have been investigated. Maximum decolorization efficiency was observed at 0.2 and 0.4 mmol/L hydrogen peroxide, 2.5 mmol/L veratryl alcohol and pH 5.0 after a 1-h reaction, using 50 ppm of dyes and 9.96 mkat/L of enzyme.

• MeSH
• Azadirachta MeSH
• barva MeSH
• barvicí látky metabolismus   MeSH
• benzylalkoholy metabolismus   MeSH
• biodegradace MeSH
• bioreaktory MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média MeSH
• lignin metabolismus   MeSH
• peroxidasy metabolismus   MeSH
• Phanerochaete enzymologie   MeSH
• substrátová specifita MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• barvicí látky MeSH
• benzylalkoholy MeSH
• kultivační média MeSH
• lignin peroxidase MeSH Prohlížeč
• lignin MeSH
• manganese peroxidase MeSH Prohlížeč
• peroxidasy MeSH
• veratryl alcohol MeSH Prohlížeč

The ability of a Brazilian strain of Pleurotus pulmonarius to decolorize structurally different synthetic dyes (including azo, triphenylmethane, heterocyclic and polymeric dyes) was investigated in solid and submerged cultures. Both were able to decolorize completely or partially 8 of 10 dyes (Amido Black, Congo Red, Trypan Blue, Methyl Green, Remazol Brilliant Blue R, Methyl Violet, Ethyl Violet, Brilliant Cresyl Blue). No decolorization of Methylene Blue and Poly R 478 was observed. Of the four phenol-oxidizing enzymes tested in culture filtrates (lignin peroxidase, manganese peroxidase, aryl alcohol oxidase, laccase), P. pulmonarius produced only laccase. Both laccase activity and dye decolorization were related to glucose and ammonium starvation or to induction by ferulic acid. The decolorization in vivo was tested using three dyes--Remazol Brilliant Blue R, Trypan Blue and Methyl Green. All of them were completely decolorized by crude extracellular extracts. Decolorization and laccase activity were equally affected by pH and temperature. Laccase can thus be considered to be the major enzyme involved in the ability of P. pulmonarius to decolorize industrial dyes.

• MeSH
• barvicí látky metabolismus   MeSH
• fermentace MeSH
• lakasa MeSH
• oxidoreduktasy biosyntéza   metabolismus   MeSH
• Pleurotus enzymologie   metabolismus   MeSH
• průmyslová mikrobiologie * MeSH
• tyrosinasa biosyntéza   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Brazílie MeSH
• Názvy látek
• barvicí látky MeSH
• lakasa MeSH
• oxidoreduktasy MeSH
• tyrosinasa MeSH

Out of a number of white-rot fungal cultures, strains of Irpex lacteus and Pleurotus ostreatus were selected for degradation of 7 three- and four-ring unsubstituted aromatic hydrocarbons (PAH) in two contaminated industrial soils. Respective data for removal of PAH in the two industrial soils by I. lacteus were: fluorene (41 and 67%), phenanthrene (20 and 56%), anthracene (29 and 49%), fluoranthene (29 and 57%), pyrene (24 and 42%), chrysene (16 and 32%) and benzo[a]anthracene (13 and 20%). In the same two industrial soils P. ostreatus degraded the PAH with respective removal figures of fluorene (26 and 35%), phenanthrene (0 and 20%), anthracene (19 and 53%), fluoranthene (29 and 31%), pyrene (22 and 42%), chrysene (0 and 42%) and benzo[a]anthracene (0 and 13%). The degradation of PAH was determined against concentration of PAH in non-treated contaminated soils after 14 weeks of incubation. The fungal degradation of PAH in soil was studied simultaneously with ecotoxicity evaluation of fungal treated and non-treated contaminated soils. Compared to non-treated contaminated soil, fungus-treated soil samples indicated decrease in inhibition of bioluminescence in luminescent bacteria (Vibrio fischerii) and increase in germinated mustard (Brassica alba) seeds.

• MeSH
• biodegradace * MeSH
• houby enzymologie   metabolismus   MeSH
• látky znečišťující půdu metabolismus   MeSH
• Pleurotus enzymologie   metabolismus   MeSH
• polycyklické aromatické uhlovodíky chemie   metabolismus   MeSH
• půdní mikrobiologie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• látky znečišťující půdu MeSH
• polycyklické aromatické uhlovodíky MeSH