Nejvíce citovaný článek - PubMed ID 12800501
Monitoring the growth and stress responses of yeast cells by two-dimensional fluorescence spectroscopy: first results
This review summarizes principles and current stage of development of fiber-optic chemical sensors (FOCS) and biosensors (FOBS). Fiber optic sensor (FOS) systems use the ability of optical fibers (OF) to guide the light in the spectral range from ultraviolet (UV) (180 nm) up to middle infrared (IR) (10 μm) and modulation of guided light by the parameters of the surrounding environment of the OF core. The introduction of OF in the sensor systems has brought advantages such as measurement in flammable and explosive environments, immunity to electrical noises, miniaturization, geometrical flexibility, measurement of small sample volumes, remote sensing in inaccessible sites or harsh environments and multi-sensing. The review comprises briefly the theory of OF elaborated for sensors, techniques of fabrications and analytical results reached with fiber-optic chemical and biological sensors.
- Klíčová slova
- chemical sensor, enzymatic sensor, fiber-optic sensor, immobilization of biologicals, tapered optical fiber, whole cell biosensor,
- MeSH
- biosenzitivní techniky přístrojové vybavení MeSH
- biotest přístrojové vybavení MeSH
- chemické techniky analytické přístrojové vybavení MeSH
- design vybavení MeSH
- lidé MeSH
- optická vlákna * MeSH
- technologie optických vláken přístrojové vybavení MeSH
- Check Tag
- lidé MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- přehledy MeSH
This work presents a yeast-cell vitality-assessment method based on on-line intracellular fluorescence measurement. The intracellular NAD(P)H fluorescence of a cell suspension is recorded during transition from aerobic to anaerobic conditions and the output signal is evaluated as a measure of yeast vitality (quality). This fluorescence method showed a highly satisfactory correlation with even low dead cell numbers where the acidification power test could not be applied.
- MeSH
- aerobióza MeSH
- anaerobióza MeSH
- fluorescenční spektrometrie metody MeSH
- mikrobiální viabilita * MeSH
- NAD metabolismus MeSH
- NADP metabolismus MeSH
- Saccharomyces cerevisiae růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- hodnotící studie MeSH
- práce podpořená grantem MeSH
- Názvy látek
- NAD MeSH
- NADP MeSH
