The wood-decomposing fungal species Antrodia macra, A. pulvinascens, Ceriporiopsis aneirina, C. resinascens and Dichomitus albidofuscus were determined for production of laccase (LAC), Mn peroxidase (MnP), lignin peroxidase (LiP), endo-l,4-P-beta-glucanase, endo-l,4-beta-xylanase, cellobiohydrolase, 1,4-beta-glucosidase and 1,4-beta-xylosidase. The results confirmed the brown-rot mode of Antrodia spp. which did not produce the activity of LAC and MnP. The remaining species performed detectable activity of both enzymes while no strain produced LiP. Significant inhibition of LAC production by high nitrogen was found in all white-rot species while only MnP of D. albidofuscus was regulated in the same way. The endoglucanase and endoxylanase activities of white-rotting species were inhibited by glucose in the medium while those of Antrodia spp. were not influenced by glucose concentration. The regulation of enzyme activity and bio-mass production can vary even within a single fungal genus.

• MeSH
• Basidiomycota enzymologie   izolace a purifikace   metabolismus   MeSH
• dřevo mikrobiologie   MeSH
• dusík metabolismus   MeSH
• fungální proteiny metabolismus   MeSH
• lakasa genetika   metabolismus   MeSH
• lignin metabolismus   MeSH
• peroxidasy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• dusík MeSH
• fungální proteiny MeSH
• lakasa MeSH
• lignin MeSH
• lignocellulose MeSH Prohlížeč
• manganese peroxidase MeSH Prohlížeč
• peroxidasy MeSH

The extracellular ligninolytic enzyme system of Pleurotus laciniatocrenatus, grown under different culture conditions, was characterized and the ability of this strain to degrade different components of Eucalyptus globulus wood was determined. In shaken liquid cultures grown on a C-limited medium supplemented with yeast extract (0.1%) and peptone (0.5%), the fungus produced extracellular aryl-alcohol oxidase (Aao), laccase (Lac), manganese-dependent peroxidase (MnP) and manganese-independent peroxidase (MiP) activities, their maximum levels being, respectively, about 600, 50, 1360, and 920 pkat/mL. The supplementation of 1 mmol/L vanillic acid and 150 micromol/L CuSO4 produced an increase of Lac activity levels up to 4-fold and 68.3-fold, respectively. No significant differences were found in the levels of the other ligninolytic enzyme activities when compared to the basal medium. Solid-state fermentation cultures on E. globulus wood chips revealed Lac and MiP activities. These cultures showed degradative activity on lignin and lipophilic wood extractives.

• MeSH
• biotechnologie metody   MeSH
• dřevo MeSH
• Eucalyptus metabolismus   MeSH
• kultivační média MeSH
• lakasa metabolismus   MeSH
• lignin metabolismus   MeSH
• peroxidasa metabolismus   MeSH
• Pleurotus enzymologie   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Argentina MeSH
• Názvy látek
• kultivační média MeSH
• lakasa MeSH
• lignin MeSH
• peroxidasa MeSH

To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.

• MeSH
• alfa-amylasy izolace a purifikace   metabolismus   MeSH
• Basidiomycota klasifikace   enzymologie   metabolismus   MeSH
• beta-glukosidasa izolace a purifikace   metabolismus   MeSH
• biodegradace MeSH
• celulasa izolace a purifikace   metabolismus   MeSH
• endo-1,4-beta-xylanasy izolace a purifikace   metabolismus   MeSH
• houby šii-take enzymologie   MeSH
• hydrolasy izolace a purifikace   metabolismus   MeSH
• lakasa izolace a purifikace   metabolismus   MeSH
• Pleurotus enzymologie   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• alfa-amylasy MeSH
• beta-glukosidasa MeSH
• celulasa MeSH
• endo-1,4-beta-xylanasy MeSH
• hydrolasy MeSH
• lakasa MeSH

A new simple method used to eliminate polysaccharides that cause problems during DNA isolation was established for 6 different white-rot fungi using 1% hexadecyltrimethylammonium bromide (CTAB) as wash buffer and followed by centrifugation. Variation in the DNA yield and quality was ascertained using precipitating agents, detergents and cell-wall-hydrolyzing chitinase. Considerable amount of exopolysaccharides from fungal biomass was removed with the use of 1% CTAB wash buffer followed by centrifugation. The DNA varied in terms of yield and quality. For the DNA extraction use of 2% SDS in extraction buffer worked best for Pycnoporus cinnabarinus, Cyathus bulleri, Cyathus striatus and Cyathus stercoreus, while 2% CTAB worked best for Phanerochaete chrysosporium and Pleurotus ostreatus. Elimination of phenol and use of absolute ethanol for precipitating DNA resulted in good yield and quality of DNA. This DNA was amenable to restriction endonuclease digestion.

• MeSH
• Basidiomycota chemie   MeSH
• cetrimoniové sloučeniny chemie   MeSH
• cetrimonium MeSH
• chitinasy metabolismus   MeSH
• detergenty MeSH
• DNA fungální analýza   izolace a purifikace   metabolismus   MeSH
• fenol chemie   MeSH
• frakční precipitace MeSH
• mykologie metody   MeSH
• Phanerochaete chemie   MeSH
• Pleurotus chemie   MeSH
• polysacharidy chemie   MeSH
• restrikční enzymy metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• cetrimoniové sloučeniny MeSH
• cetrimonium MeSH
• chitinasy MeSH
• detergenty MeSH
• DNA fungální MeSH
• fenol MeSH
• polysacharidy MeSH
• restrikční enzymy MeSH