Nejvíce citovaný článek - PubMed ID 14149848
RELATIONSHIP BETWEEN THE CARBOHYDRATE METABOLISM OF STREPTOMYCES AUREOFACIENS AND THE BIOSYNTHESIS OF CHLORTETRACYCLINE. II. THE EFFECT OF BENZYL THIOCYANATE ON THE RESPIRATION OF WASHED MYCELIUM OF STREPTOMYCES AUREOFACIENS
Cell protein profiles of submerged cultures of Streptomyces aureofaciens cultivated in the absence or presence of 12 microM benzyl thiocyanate (BT) were analyzed by one-dimensional SDS polyacrylamide gel electrophoresis. Substantial increase in the intensity of the 13, 35, 37, 60, and 100 kDa protein bands was observed in cultures treated with BT. Similar increase in the 35, 37, and 60 kDa bands was found in a mutant blocked in the last chlortetracycline biosynthesis step. Effect of BT on the solid medium-grown cultures was also observed, with a more intensive substrate mycelium pigmentation and alteration in the spore size and shape as the most characteristic features. Earlier studies of BT effect involving those on the stimulation of chlortetracycline biosynthesis are summarized and a possible signal-transducing mechanism is discussed from the point of view of adaptation of S. aureofaciens to the uncoupling of oxidative phosphorylation.
- MeSH
- bakteriální proteiny metabolismus MeSH
- elektronová mikroskopie MeSH
- Streptomyces aureofaciens účinky léků metabolismus ultrastruktura MeSH
- tetracykliny biosyntéza MeSH
- thiokyanatany farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- práce podpořená grantem MeSH
- Názvy látek
- bakteriální proteiny MeSH
- benzyl thiocyanate MeSH Prohlížeč
- tetracykliny MeSH
- thiokyanatany MeSH
The level of anhydrotetracyline oxygenase (an enzyme catalyzing the penultimate reaction in the biosynthesis of tetracyline) in Streptomyces aureofaciens was substantially influenced by the amount of inorganic phosphate and by the presence of benzyl thiocyanate in the cultivation medium. Phosphate decreased the specific activity of the enzyme, particularly when added to a growing culture. On the other hand, benzyl thiocyanate increased the specific activity of the enzyme. Its effect was most conspicuous in the growth phase. The effect of benzyl thiocyanate was more pronounced in the low-production strain than in the producing variant. Inorganic phosphate and benzyl thiocyanate did not influence the enzyme activity in vitro. Phosphate added to the growing cultures was readily absorbed by the cells. During this time the enzyme synthesis was repressed, derepresion occurred only after exhaustion of phosphate from the medium. The stimulatory effect of benzyl thiocyanate on the enzyme synthesis was not reversed by the inorganic phosphate added.
- MeSH
- fosfáty farmakologie MeSH
- oxygenasy metabolismus MeSH
- Streptomyces enzymologie MeSH
- tetracykliny metabolismus MeSH
- thiokyanatany farmakologie MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- 4-epianhydrotetracycline MeSH Prohlížeč
- anhydrotetracycline oxygenase MeSH Prohlížeč
- benzyl thiocyanate MeSH Prohlížeč
- fosfáty MeSH
- oxygenasy MeSH
- tetracykliny MeSH
- thiokyanatany MeSH
- MeSH
- Acremonium metabolismus MeSH
- adenosintrifosfát metabolismus MeSH
- antibakteriální látky biosyntéza MeSH
- druhová specificita MeSH
- enzymová represe MeSH
- fermentace MeSH
- fosfáty metabolismus MeSH
- glukosa metabolismus MeSH
- metabolismus sacharidů MeSH
- Penicillium metabolismus MeSH
- Streptomyces metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- adenosintrifosfát MeSH
- antibakteriální látky MeSH
- fosfáty MeSH
- glukosa MeSH
- MeSH
- antibakteriální látky biosyntéza MeSH
- Bacteria růst a vývoj metabolismus MeSH
- bakteriální proteiny metabolismus MeSH
- bakteriální RNA metabolismus MeSH
- DNA bakterií metabolismus MeSH
- DNA metabolismus MeSH
- houby růst a vývoj metabolismus MeSH
- RNA metabolismus MeSH
- spory bakteriální fyziologie MeSH
- Streptomyces růst a vývoj metabolismus MeSH
- Publikační typ
- časopisecké články MeSH
- přehledy MeSH
- Názvy látek
- antibakteriální látky MeSH
- bakteriální proteiny MeSH
- bakteriální RNA MeSH
- DNA bakterií MeSH
- DNA MeSH
- RNA MeSH
- Klíčová slova
- CARBOHYDRATE METABOLISM *, CHLORTETRACYCLINE *, FRUCTOSE *, GLUCOSE *, METABOLISM *, PHARMACOLOGY *, STREPTOMYCES *, SUCROSE *, THIOCYANATES *,
- MeSH
- chlortetracyklin * MeSH
- farmakologie * MeSH
- fruktosa * MeSH
- glukosa * MeSH
- metabolismus sacharidů * MeSH
- metabolismus * MeSH
- sacharosa * MeSH
- Streptomyces aureofaciens * MeSH
- Streptomyces * MeSH
- thiokyanatany * MeSH
- Publikační typ
- časopisecké články MeSH
- Názvy látek
- benzyl thiocyanate MeSH Prohlížeč
- chlortetracyklin * MeSH
- fruktosa * MeSH
- glukosa * MeSH
- sacharosa * MeSH
- thiokyanatany * MeSH
