The treatment of three patients suffering from chronic bacterial prostatitis who were qualified for an experimental phage therapy protocol managed at the Phage Therapy Unit in Wrocław is described. They had previously been treated unsuccessfully with long-term targeted antibiotics, autovaccines, and laser biostimulation. Rectal application of phage lysates targeted against Enterococcus faecalis cultured from the prostatic fluid gave encouraging results regarding bacterial eradication, abatement of clinical symptoms of prostatitis, and lack of early disease recurrence.

• MeSH
• bakteriofágy chemie   fyziologie   MeSH
• biologická terapie * MeSH
• dospělí MeSH
• Enterococcus faecalis izolace a purifikace   virologie   MeSH
• grampozitivní bakteriální infekce mikrobiologie   terapie   MeSH
• lidé MeSH
• prostatitida mikrobiologie   terapie   MeSH
• Check Tag
• dospělí MeSH
• lidé MeSH
• mužské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• kazuistiky MeSH

Effect of pH on production of extracellular virulence factors of Pseudomonas aeruginosa grown on catheter in biofilm was determined. Alginate and proteinase production was higher at pH 8; in contrast, siderophores (pyochelin and pyoverdin) were synthesized more intensively at pH 5.

• MeSH
• algináty metabolismus   MeSH
• bakteriální proteiny metabolismus   MeSH
• biofilmy růst a vývoj   MeSH
• faktory virulence biosyntéza   MeSH
• koncentrace vodíkových iontů MeSH
• kultivační média speciální chemie   MeSH
• kyselina glukuronová metabolismus   MeSH
• kyseliny hexuronové metabolismus   MeSH
• lidé MeSH
• moč mikrobiologie   MeSH
• proteasy metabolismus   MeSH
• pseudomonádové infekce mikrobiologie   MeSH
• Pseudomonas aeruginosa růst a vývoj   metabolismus   patogenita   fyziologie   MeSH
• siderofory metabolismus   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• algináty MeSH
• bakteriální proteiny MeSH
• faktory virulence MeSH
• kultivační média speciální MeSH
• kyselina glukuronová MeSH
• kyseliny hexuronové MeSH
• proteasy MeSH
• siderofory MeSH

One hundred and four enterotoxin producing Escherichia coli strains of wide geographical origin were tested for the expression of curli fimbriae by transmission electronmicroscopy and by ELISA using curli-specific antibodies, as well as for the presence of curli-specific gene sequences by PCR. All isolates, irrespective of the production of the fimbriae, carried sequences specific for the structure (csgA) and for one of the regulator genes (crl) of curli expression, respectively. Curli fimbriae were detected in 56 strains (53.8 %). Thirty-six strains expressed curli only when growing at 30 degrees C, 4 isolates were weakly curliated at 37 degrees C only, while on 16 strains curli was observed at both temperatures. On isolates carrying curli at both temperatures the expression of the fimbria was significantly stronger at 30 degrees C than at 37 degrees C. Curli proficiency significantly, but not completely, correlated with the binding of the Congo Red dye. The expression of curli did not confer epithelial cell invasiveness to ETEC strains but, once expressed at 30 degrees C, it facilitated the adherence of the bacteria to plastic surfaces. Curli present in more than half of the ETEC strains and expressed preferentially at low temperatures could be a factor facilitating the environmental survival of this food- and water-borne pathogen.

• MeSH
• bakteriální adheze MeSH
• bakteriální fimbrie * ultrastruktura   MeSH
• bakteriální geny MeSH
• bakteriální proteiny analýza   MeSH
• ELISA MeSH
• enterotoxiny biosyntéza   MeSH
• Escherichia coli genetika   izolace a purifikace   ultrastruktura   MeSH
• Kongo červeň metabolismus   MeSH
• proteiny z Escherichia coli chemie   genetika   imunologie   MeSH
• regulace genové exprese u bakterií MeSH
• regulační geny MeSH
• teplota MeSH
• transmisní elektronová mikroskopie MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• bakteriální proteiny MeSH
• Crl protein, Bacteria MeSH Prohlížeč
• csgA protein, E coli MeSH Prohlížeč
• enterotoxiny MeSH
• Kongo červeň MeSH
• proteiny z Escherichia coli MeSH

The resistance to antibiotics and the distribution of virulence factors in enterococci isolated from traditional Slovak sheep cheese bryndza was compared with strains from human infections. The occurrence of 4 enterococcal species was observed in 117 bryndza-cheese isolates. The majority of strains were identified as E. faecium (76 %) and E. faecalis (23 %). Several strains of E. durans and 1 strain of E. hirae were also present. More than 90 % of strains isolated from 109 clinical enterococci were E. faecalis, the rest belonged to E. faecium. The resistance to 6 antimicrobial substances (ampicillin, ciprofloxacin, higher concentration of gentamicin, nitrofurantoin, tetracycline and vancomycin) was tested in clinical and food enterococci. A higher level of resistance was found in clinical than in food strains and E. faecium had a higher resistance than E. faecalis; no resistance to vancomycin was detected. The occurrence of 3 virulence-associated genes, cylA (coding for hemolysin), gelE (coding for gelatinase) and esp (coding for surface protein) was monitored. Differences were found in the distribution of cylA gene between clinical and bryndza-cheese E. faecalis strains; in contrast to clinical strains (45 %), cylA gene was detected in 22 % of food isolates. The distribution of 2 other virulence factors, gelE and esp, was not significantly different in the two groups of E. faecalis strains. cylA and gelE genes were not detected in E. faecium but more than 70 % of clinical E. faecium were positive for esp, even thought none of the 79 E. faecium cheese isolates contained this gene.

• MeSH
• antibakteriální látky farmakologie   MeSH
• bakteriální léková rezistence * MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• Enterococcus faecalis účinky léků   patogenita   MeSH
• Enterococcus faecium účinky léků   patogenita   MeSH
• Enterococcus klasifikace   účinky léků   izolace a purifikace   patogenita   MeSH
• faktory virulence genetika   metabolismus   MeSH
• grampozitivní bakteriální infekce mikrobiologie   MeSH
• lidé MeSH
• mikrobiální testy citlivosti MeSH
• ovce MeSH
• sýr mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Geografické názvy
• Slovenská republika MeSH
• Názvy látek
• antibakteriální látky MeSH
• bakteriální proteiny MeSH
• faktory virulence MeSH

Exoproteinase production was demonstrated in 64 clinical isolates of S. marcescens. A significant relationship was found between the site of origin (autopsy material, hemocultures, various other sources), proteinase activity, and LD50 of the analyzed isolates. The number of exoproteinases varied during a 14-h incubation in batch cultures; the most frequently found was a 57.5-kDa proteinase which was observed in all analyzed strains. The exoproteinase production was shown to be related to strain virulence.

• MeSH
• exopeptidasy metabolismus   MeSH
• lidé MeSH
• Serratia marcescens enzymologie   patogenita   fyziologie   MeSH
• virulence MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• exopeptidasy MeSH

A total of 5 Bifidobacterium spp. isolated from pig and children' feces and 6 Lactobacillus spp. from chicken feces were examined for expression of aggregation, cell surface hydrophobicity (CSH) and adherence to intestinal mucin. Co-aggregation activity was seen in 3 strains of auto-aggregative bifidobacteria and 4 auto-aggregative strains of Lactobacillus spp. with 2 enterohemorrhagic E. coli (O157). CSH correlated with Lactobacillus auto-aggregating activity and adherence to mucin but the correlation between Bifidobacterium adherence to mucin and CSH was not confirmed.

• MeSH
• bakteriální adheze * MeSH
• Bifidobacterium izolace a purifikace   fyziologie   MeSH
• Escherichia coli O157 fyziologie   MeSH
• feces mikrobiologie   MeSH
• hydrofobní a hydrofilní interakce MeSH
• kur domácí mikrobiologie   MeSH
• Lactobacillus izolace a purifikace   fyziologie   MeSH
• lidé MeSH
• muciny metabolismus   MeSH
• prasata mikrobiologie   MeSH
• zvířata MeSH
• Check Tag
• lidé MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• muciny MeSH

Planktonic and biofilm cells of a clinical urinary isolate of P. aeruginosa were compared in vitro for their ability to adhere to uroepithelial cells, interaction with macrophages, and for production of virulence factors like extracellular proteinase, elastase, hemolysin, phospholipase C and pyochelin. Biofilm cells showed increased adherence to UECs, which was coupled with reduced uptake and intracellular killing by macrophages. Overall there was a decrease in production of extracellular products by biofilm cells. Comparing the two cell forms for their ability to establish infection in an ascending model of acute pyelonephritis, significant enhancement of renal bacterial load, as well as more pronounced renal pathology developed with biofilm cells.

• MeSH
• biofilmy * MeSH
• myši inbrední BALB C MeSH
• myši MeSH
• plankton patogenita   MeSH
• Pseudomonas aeruginosa patogenita   MeSH
• pyelonefritida etiologie   MeSH
• virulence MeSH
• zvířata MeSH
• Check Tag
• myši MeSH
• ženské pohlaví MeSH
• zvířata MeSH
• Publikační typ
• časopisecké články MeSH