In recent decades, the non-thermal plasma, i.e. partially or completely ionized gas produced by electric discharges at ambient temperature, has become of interest for its microbiocidal properties with potential of use in the food industry or medicine. Recently, this interest focuses not only on the planktonic forms of microorganisms but also on their biofilms. The works in this interdisciplinary field are summarized in this review. The wide range of biofilm-plasma interactions is divided into studies of general plasma action on bacteria, on biofilm and on its oral and dental application; a short overview of plasma instrumentation is also included. In addition, not only biofilm combating but also an important area of biofilm prevention is discussed. Various DC discharges of the point-to-plane type. Author's photograph, published in Khun et al. (Plasma Sources Sci Technol 27:065002, 2018).

• Klíčová slova
• Biofilm inactivation, Biofilm prevention, Dental and oral, Non-thermal plasma production, Wound healing,
• MeSH
• Bacteria růst a vývoj   účinky záření   MeSH
• biofilmy růst a vývoj   účinky záření   MeSH
• dezinfekce přístrojové vybavení   metody   MeSH
• hojení ran MeSH
• houby účinky záření   MeSH
• plazmové plyny farmakologie   MeSH
• potravinářský průmysl MeSH
• zubní materiály farmakologie   MeSH
• Publikační typ
• časopisecké články MeSH
• přehledy MeSH
• Názvy látek
• plazmové plyny MeSH
• zubní materiály MeSH

The effect of the yeast cell-death inducing agents, Bax and acetic acid, on mitochondrial structure of Schizosaccharomyces pombe was studied. Comparison of mitochondrial structures in cells grown on different substrates and visualized with different probes revealed variations in their morphology. Cells grown on respiratory C sources as well as in the presence of antimycin A exhibited punctuated mitochondria when visualized with mitochondrially targeted green fluorescent protein, while they still appeared as tubular structures when stained with DiOC6(3). Both expression of Bax and acetic acid treatment induced fragmentation and aggregation of mitochondrial network, which could be prevented by coexpression of Bcl-XL. Aberrant mitochondrial morphology generated by either Bax or acetic acid was not accompanied with the loss of mitochondrial genome (mtDNA), indicating that alterations of mitochondrial morphology following death stimuli follow different mechanisms than those involved in mitochondrial inheritance mutants.

• MeSH
• buněčná smrt účinky léků   fyziologie   MeSH
• DNA fungální genetika   MeSH
• fluorescenční mikroskopie MeSH
• karbocyaniny chemie   MeSH
• kyselina octová farmakologie   MeSH
• mitochondriální DNA genetika   MeSH
• mitochondrie účinky léků   fyziologie   ultrastruktura   MeSH
• plazmidy genetika   MeSH
• protein X asociovaný s bcl-2 biosyntéza   genetika   fyziologie   MeSH
• Schizosaccharomyces účinky léků   genetika   fyziologie   MeSH
• Southernův blotting MeSH
• transformace genetická MeSH
• zelené fluorescenční proteiny biosyntéza   genetika   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• 3,3'-dihexyl-2,2'-oxacarbocyanine MeSH Prohlížeč
• DNA fungální MeSH
• karbocyaniny MeSH
• kyselina octová MeSH
• mitochondriální DNA MeSH
• protein X asociovaný s bcl-2 MeSH
• zelené fluorescenční proteiny MeSH