We evaluated the suitability of various primers for the RAPD (random amplified polymorphic DNA) accurate species identification and strain typing of Aspergillus clinical isolates. Five primers described previously were tested for their discriminatory power in three Aspergillus species (A. fumigatus, A. niger agg. and A. flavus - 23 clinical isolates and 2 reference strains). Clustering of RAPD fingerprints corresponded well with the identification based on morphological features. All isolates were resolved as different strains using the primer R108 and the RAPD protocol optimized for a Robocycler thermal cycler. RAPD with the primer R108 thus can be considered to be a valuable, simple and powerful tool for identification and strain delineation of Aspergillus spp.

• MeSH
• Aspergillus flavus klasifikace   MeSH
• Aspergillus fumigatus klasifikace   MeSH
• Aspergillus niger klasifikace   MeSH
• aspergilóza diagnóza   mikrobiologie   MeSH
• DNA fingerprinting metody   MeSH
• DNA primery MeSH
• lidé MeSH
• mykologické určovací techniky metody   MeSH
• reprodukovatelnost výsledků MeSH
• technika náhodné amplifikace polymorfní DNA metody   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA primery MeSH

The relationship between inflammatory bowel disease and microorganisms was evaluated. The presence of Candida albicans-specific IgM and IgG antibodies in serum samples and the presence of C. albicans in stool and colonal mucosa samples of the patients did not exhibit any significant difference between 21 patients in active stage and 15 patients in remission of ulcerative colitis (UC) (compared with 19 control patients). The invasion of yeast cells to the colonal mucosa was demonstrated by detecting C. albicans DNA using specific PCon1, PCon2, and PspA2 primers in PCR assay. Eighteen of 36 patients (50%) were found to be DNA positive while in 19 controls only 4 (21%) were found to be positive. The presence of DNA in the association of the positive serological reactivity is suggested as an important diagnostic marker of UC.

• MeSH
• biologické modely MeSH
• Candida albicans genetika   imunologie   izolace a purifikace   patogenita   MeSH
• DNA fungální analýza   genetika   MeSH
• feces mikrobiologie   MeSH
• imunoglobulin G krev   MeSH
• imunoglobulin M krev   MeSH
• lidé MeSH
• polymerázová řetězová reakce MeSH
• protilátky fungální krev   MeSH
• sekvence nukleotidů MeSH
• střevní sliznice mikrobiologie   MeSH
• studie případů a kontrol MeSH
• ulcerózní kolitida etiologie   imunologie   mikrobiologie   patologie   MeSH
• Check Tag
• lidé MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• DNA fungální MeSH
• imunoglobulin G MeSH
• imunoglobulin M MeSH
• protilátky fungální MeSH

Secreted aspartyl proteinase (Sap) distribution among different C. albicans isolates was determined using SAP-specific primers in polymerase chain reaction (PCR) assay. SAP1, SAP2, and SAP3 were detected in 13 of 40 (32.5%), SAP4 in 38/40 (95%), SAP5 were detected in 30/40 (75%), SAP6 in 23/40 (57.5%) of C. albicans strains isolated from blood cultures. SAP1-SAP3 were detected in 37 of 40 (92.5%), SAP4 were detected in 3/40 (7.5%), SAP5 in 3/40 (7.5%), SAP6 in 5/40 (12.5%) of C. albicans strains isolated from vaginal swab cultures. Sap1, Sap2 and Sap3 isoenzymes were found to be related to the vaginopathic potential of C. albicans; Sap4, Sap5 and Sap6 isoenzymes were found to be correlated with systemic infections.

• MeSH
• aspartátové endopeptidasy analýza   genetika   MeSH
• Candida albicans enzymologie   genetika   izolace a purifikace   MeSH
• DNA fungální analýza   MeSH
• fungální proteiny analýza   genetika   MeSH
• geny hub MeSH
• izoenzymy antagonisté a inhibitory   genetika   MeSH
• kandidóza mikrobiologie   MeSH
• krev mikrobiologie   MeSH
• lidé MeSH
• polymerázová řetězová reakce metody   MeSH
• vagina mikrobiologie   MeSH
• Check Tag
• lidé MeSH
• mužské pohlaví MeSH
• ženské pohlaví MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• aspartátové endopeptidasy MeSH
• DNA fungální MeSH
• fungální proteiny MeSH
• izoenzymy MeSH
• SAP1 protein, Candida albicans MeSH Prohlížeč
• SAP2 protein, Candida MeSH Prohlížeč
• SAP3 protein, Candida albicans MeSH Prohlížeč
• SAP4 protein, Candida albicans MeSH Prohlížeč
• SAP5 protein, Candida albicans MeSH Prohlížeč