In this work, the effect of moderate electromagnetic fields (2.5, 10, and 15 mT) was studied using an immersed coil inserted directly into a bioreactor on batch cultivation of yeast under both aerobic and anaerobic conditions. Throughout the cultivation, parameters, including CO2 levels, O2 saturation, nitrogen consumption, glucose uptake, ethanol production, and yeast growth (using OD 600 measurements at 1-h intervals), were analysed. The results showed that 10 and 15 mT magnetic fields not only statistically significantly boosted and sped up biomass production (by 38-70%), but also accelerated overall metabolism, accelerating glucose, oxygen, and nitrogen consumption, by 1-2 h. The carbon balance analysis revealed an acceleration in ethanol and glycerol production, albeit with final concentrations by 22-28% lower, with a more pronounced effect in aerobic cultivation. These findings suggest that magnetic fields shift the metabolic balance toward biomass formation rather than ethanol production, showcasing their potential to modulate yeast metabolism. Considering coil heating, opting for the 10 mT magnetic field is preferable due to its lower heat generation. In these terms, we propose that magnetic field can be used as novel tool to increase biomass yield and accelerate yeast metabolism.

• Klíčová slova
• Aerobic, Anaerobic, Batch fermentation, Biomass, Magnetic field, Metabolism acceleration, Yeast,
• MeSH
• aerobióza MeSH
• anaerobióza MeSH
• biomasa * MeSH
• bioreaktory mikrobiologie   MeSH
• dusík metabolismus   MeSH
• ethanol * metabolismus   MeSH
• fermentace * MeSH
• glukosa metabolismus   MeSH
• glycerol metabolismus   MeSH
• kyslík metabolismus   MeSH
• magnetické pole * MeSH
• Saccharomyces cerevisiae * metabolismus   růst a vývoj   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• dusík MeSH
• ethanol * MeSH
• glukosa MeSH
• glycerol MeSH
• kyslík MeSH

Normal or excessive oxidative metabolism in organisms is essential in physiological and pathophysiological processes, respectively. Therefore, monitoring of biological oxidative processes induced by the chemical or physical stimuli is nowadays of extreme importance due to the environment overloaded with various physicochemical factors. Current techniques typically require the addition of chemical labels or light illumination, which perturb the samples to be analyzed. Moreover, the current techniques are very demanding in terms of sample preparation and equipment. To alleviate these limitations, we propose a label-free monitoring tool of oxidation based on biological autoluminescence (BAL). We demonstrate this tool on Saccharomyces cerevisiae cell culture. We showed that BAL can be used to monitor chemical perturbation of yeast due to Fenton reagents initiated oxidation-the BAL intensity changes with hydrogen peroxide concentration in a dose-dependent manner. Furthermore, we also showed that BAL reflects the effects of low-frequency magnetic field on the yeast cell culture, where we observed a disturbance of the BAL kinetics in the exposed vs. control case. Our results contribute to the development of novel techniques for label-free, real-time, noninvasive monitoring of oxidative processes and approaches for their modulation.

• MeSH
• celulosa analogy a deriváty   farmakologie   MeSH
• fixní kombinace léků MeSH
• kultivační techniky MeSH
• luminiscence * MeSH
• oxidace-redukce účinky léků   MeSH
• povidon farmakologie   MeSH
• Saccharomyces cerevisiae cytologie   účinky léků   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• celulosa MeSH
• fixní kombinace léků MeSH
• hyetellose, povidone drug combination MeSH Prohlížeč
• povidon MeSH