Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity. Among the most active species, Bosea sp. FBZP-16 demonstrated cellulolytic activity on both amorphous cellulose (CMC) and complex lignocellulose (wheat straw) and was selected for whole-genomic sequencing. The genome sequencing revealed the presence of a complex enzymatic machinery required for organic matter decomposition. Analysis of the enzyme-encoding genes indicated that multiple genes for endoglucanase, xylanase, β-glucosidase and β-mannosidase are present in the genome with enzyme activities displayed by the bacterium, while other enzymes, such as certain cellobiohydrolases, were not detected at the genomic level. This indicates that a combination of functional screening of bacterial cultures with the use of genome-derived information is important for the prediction of potential enzyme production. These results provide insight into their possible exploitation for the production of fuels and chemicals derived from plant biomass.

• Keywords
• Bosea, Cellulases, Enzyme assays, Genome sequencing, Hemicellulases,
• MeSH
• Actinobacteria genetics   isolation & purification   MeSH
• Bacterial Proteins genetics   metabolism   MeSH
• Cellulase genetics   metabolism   MeSH
• Cellulose metabolism   MeSH
• Phylogeny MeSH
• Glycoside Hydrolases genetics   metabolism   MeSH
• Lignin metabolism   MeSH
• Proteobacteria genetics   isolation & purification   MeSH
• Soil MeSH
• Soil Microbiology MeSH
• Rhizobiaceae enzymology   genetics   isolation & purification   MeSH
• RNA, Ribosomal, 16S genetics   MeSH
• Sequence Analysis, RNA methods   MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Bacterial Proteins MeSH
• Cellulase MeSH
• Cellulose MeSH
• Glycoside Hydrolases MeSH
• hemicellulase MeSH Browser
• Lignin MeSH
• lignocellulose MeSH Browser
• Soil MeSH
• RNA, Ribosomal, 16S MeSH

The autofluorescence (primary fluorescence, AF) of agar cultures of the brown-rot fungus Piptoporus betulinus was investigated in Zeiss Jenalumar and Nikon Eclipse 8201 fluorescence microscopes at various excitations. The strongest AF of hyphae was found in minimal medium with glucose, where the hyphae exhibited green AF at violet (450 nm) excitation and red AF at green (570 nm) excitation. Addition of metals to cultivation media led to enhanced white-blue AF in the presence of Co (at 450 nm) and yellow to yellow-brown AF at 510 nm. When cultivated with Mn and Zn, enhanced AF of intracellular content was observed. Only a weak signal was found in the presence of Cu and Fe.

• MeSH
• Coriolaceae chemistry   cytology   metabolism   MeSH
• Fluorescence * MeSH
• Microscopy, Fluorescence MeSH
• Ions metabolism   MeSH
• Metals metabolism   MeSH
• Culture Media chemistry   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Ions MeSH
• Metals MeSH
• Culture Media MeSH