Identification of bacteria that produce carbohydrolytic enzymes is extremely important given the increased demand for these enzymes in many industries. Twenty lignocellulose-degrading bacterial isolates from Algerian compost and different soils were screened for their potential to produce different enzymes involved in biomass deconstruction. Based on 16S rRNA gene sequencing, the isolates belonged to Proteobacteria and Actinobacteria. Differences among species were reflected both as the presence/absence of enzymes or at the level of enzyme activity. Among the most active species, Bosea sp. FBZP-16 demonstrated cellulolytic activity on both amorphous cellulose (CMC) and complex lignocellulose (wheat straw) and was selected for whole-genomic sequencing. The genome sequencing revealed the presence of a complex enzymatic machinery required for organic matter decomposition. Analysis of the enzyme-encoding genes indicated that multiple genes for endoglucanase, xylanase, β-glucosidase and β-mannosidase are present in the genome with enzyme activities displayed by the bacterium, while other enzymes, such as certain cellobiohydrolases, were not detected at the genomic level. This indicates that a combination of functional screening of bacterial cultures with the use of genome-derived information is important for the prediction of potential enzyme production. These results provide insight into their possible exploitation for the production of fuels and chemicals derived from plant biomass.

• Klíčová slova
• Bosea, Cellulases, Enzyme assays, Genome sequencing, Hemicellulases,
• MeSH
• Actinobacteria genetika   izolace a purifikace   MeSH
• bakteriální proteiny genetika   metabolismus   MeSH
• celulasa genetika   metabolismus   MeSH
• celulosa metabolismus   MeSH
• fylogeneze MeSH
• glykosidhydrolasy genetika   metabolismus   MeSH
• lignin metabolismus   MeSH
• Proteobacteria genetika   izolace a purifikace   MeSH
• půda MeSH
• půdní mikrobiologie MeSH
• Rhizobiaceae enzymologie   genetika   izolace a purifikace   MeSH
• RNA ribozomální 16S genetika   MeSH
• sekvenční analýza RNA metody   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• bakteriální proteiny MeSH
• celulasa MeSH
• celulosa MeSH
• glykosidhydrolasy MeSH
• hemicellulase MeSH Prohlížeč
• lignin MeSH
• lignocellulose MeSH Prohlížeč
• půda MeSH
• RNA ribozomální 16S MeSH

The effect of crop rotation and monocropping on the occurrence of bacteria with antagonistic activity toward Pythium debaryanum and Fusarium oxysporum was shown. Arthrobacter spp., fluorescent Pseudomonas spp. and actinomycetes were isolated from winter rape, sugar beet and winter barley rhizosphere and bulk soil from the plots of a long-term crop rotation experiment (18 years). The occurrence of mycoantagonistic isolates and their antibiosis level exhibited specificity for the site, crop and crop rotation. Mycoantagonistic activity was common among actinomycetes and fluorescent Pseudomonas spp. and less frequent among Arthrobacter spp. Antibiosis of fluorescent Pseudomonas spp. and Arthrobacter spp. was in general stronger against P. debaryanum than F. oxysporum. The highest percentage of antagonistic Pseudomonas spp. against P. debaryanum was in the plots of barley crop, while plots of winter rape showed higher frequency of antagonists against F. oxysporum. The highest antibiosis activity of Arthrobacter spp. against both pathogens occurred in isolates from barley and winter rape monoculture, and there were no F. oxysporum antagonists among these bacteria in sugar beet monoculture. Most of actinomycete isolates strongly inhibited growth of P. debaryanum and F. oxysporum. The percentage of mycoantagonistic actinomycetes and their antibiosis level were the highest in the 6-year crop rotation system.

• MeSH
• antibióza * MeSH
• Bacteria izolace a purifikace   MeSH
• Fusarium fyziologie   MeSH
• fyziologie bakterií MeSH
• nemoci rostlin mikrobiologie   MeSH
• půdní mikrobiologie * MeSH
• Pythium fyziologie   MeSH
• zemědělské plodiny růst a vývoj   mikrobiologie   MeSH
• zemědělství metody   MeSH
• Publikační typ
• časopisecké články MeSH
• hodnotící studie MeSH