Although EcoR124 is one of the better-studied Type I restriction-modification enzymes, it still presents many challenges to detailed analyses because of its structural and functional complexity and missing structural information. In all available structures of its motor subunit HsdR, responsible for DNA translocation and cleavage, a large part of the HsdR C terminus remains unresolved. The crystal structure of the C terminus of HsdR, obtained with a crystallization chaperone in the form of pHluorin fusion and refined to 2.45 Å, revealed that this part of the protein forms an independent domain with its own hydrophobic core and displays a unique α-helical fold. The full-length HsdR model, based on the WT structure and the C-terminal domain determined here, disclosed a proposed DNA-binding groove lined by positively charged residues. In vivo and in vitro assays with a C-terminal deletion mutant of HsdR supported the idea that this domain is involved in complex assembly and DNA binding. Conserved residues identified through sequence analysis of the C-terminal domain may play a key role in protein-protein and protein-DNA interactions. We conclude that the motor subunit of EcoR124 comprises five structural and functional domains, with the fifth, the C-terminal domain, revealing a unique fold characterized by four conserved motifs in the IC subfamily of Type I restriction-modification systems. In summary, the structural and biochemical results reported here support a model in which the C-terminal domain of the motor subunit HsdR of the endonuclease EcoR124 is involved in complex assembly and DNA binding.

• Klíčová slova
• C-terminal domain, DNA binding protein, DNA endonuclease, EcoR124, Escherichia coli (E. coli), GFP fusion, HsdR, X-ray crystallography, crystal structure, restriction-modification,
• MeSH
• biofyzikální jevy MeSH
• DNA vazebné proteiny chemie   genetika   MeSH
• Escherichia coli chemie   genetika   MeSH
• konformace proteinů MeSH
• krystalografie rentgenová MeSH
• multiproteinové komplexy chemie   genetika   MeSH
• podjednotky proteinů chemie   genetika   MeSH
• proteinové domény genetika   MeSH
• proteiny z Escherichia coli chemie   genetika   MeSH
• restrikční endonukleasy typu I chemie   genetika   MeSH
• sekvence aminokyselin MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• DNA vazebné proteiny MeSH
• endodeoxyribonuclease EcoR124I MeSH Prohlížeč
• HsdR protein, E coli MeSH Prohlížeč
• multiproteinové komplexy MeSH
• podjednotky proteinů MeSH
• proteiny z Escherichia coli MeSH
• restrikční endonukleasy typu I MeSH

Type I restriction-modification enzymes differ significantly from the type II enzymes commonly used as molecular biology reagents. On hemi-methylated DNAs type I enzymes like the EcoR124I restriction-modification complex act as conventional adenine methylases at their specific target sequences, but unmethylated targets induce them to translocate thousands of base pairs through the stationary enzyme before cleaving distant sites nonspecifically. EcoR124I is a superfamily 2 DEAD-box helicase like eukaryotic double-strand DNA translocase Rad54, with two RecA-like helicase domains and seven characteristic sequence motifs that are implicated in translocation. In Rad54 a so-called extended region adjacent to motif III is involved in ATPase activity. Although the EcoR124I extended region bears sequence and structural similarities with Rad54, it does not influence ATPase or restriction activity as shown in this work, but mutagenesis of the conserved glycine residue of its motif III does alter ATPase and DNA cleavage activity. Through the lens of molecular dynamics, a full model of HsdR of EcoR124I based on available crystal structures allowed interpretation of functional effects of mutants in motif III and its extended region. The results indicate that the conserved glycine residue of motif III has a role in positioning the two helicase domains.

• Klíčová slova
• DNA restriction enzymes, Domain interactions, Molecular mechanics, Molecular modeling, Multisubunit enzyme complex, Principal components analysis,
• MeSH
• adenosintrifosfát chemie   MeSH
• aktivace enzymů MeSH
• analýza hlavních komponent MeSH
• DNA-helikasy chemie   genetika   metabolismus   MeSH
• hydrolýza MeSH
• interakční proteinové domény a motivy * MeSH
• konformace proteinů MeSH
• multienzymové komplexy chemie   MeSH
• mutace MeSH
• podjednotky proteinů chemie   genetika   metabolismus   MeSH
• restrikční endonukleasy typu I chemie   genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• simulace molekulární dynamiky MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• adenosintrifosfát MeSH
• DNA-helikasy MeSH
• multienzymové komplexy MeSH
• podjednotky proteinů MeSH
• restrikční endonukleasy typu I MeSH

Type I restriction-modification enzymes are multisubunit, multifunctional molecular machines that recognize specific DNA target sequences, and their multisubunit organization underlies their multifunctionality. EcoR124I is the archetype of Type I restriction-modification family IC and is composed of three subunit types: HsdS, HsdM, and HsdR. DNA cleavage and ATP-dependent DNA translocation activities are housed in the distinct domains of the endonuclease/motor subunit HsdR. Because the multiple functions are integrated in this large subunit of 1,038 residues, a large number of interdomain contacts might be expected. The crystal structure of EcoR124I HsdR reveals a surprisingly sparse number of contacts between helicase domain 2 and the C-terminal helical domain that is thought to be involved in assembly with HsdM. Only two potential hydrogen-bonding contacts are found in a very small contact region. In the present work, the relevance of these two potential hydrogen-bonding interactions for the multiple activities of EcoR124I is evaluated by analysing mutant enzymes using in vivo and in vitro experiments. Molecular dynamics simulations are employed to provide structural interpretation of the functional data. The results indicate that the helical C-terminal domain is involved in the DNA translocation, cleavage, and ATPase activities of HsdR, and a role in controlling those activities is suggested.

• Klíčová slova
• DNA restriction enzymes, Domain interactions, E. coli, Molecular modeling, Multisubunit enzyme complex,
• Publikační typ
• časopisecké články MeSH

The HsdR subunit of the type I restriction-modification system EcoR124I is responsible for the translocation as well as the restriction activity of the whole complex consisting of the HsdR, HsdM and HsdS subunits, and while crystal structures are available for the wild type and several mutants, the C-terminal domain comprising approximately 150 residues was not resolved in any of these structures. Here, three fusion constructs with the GFP variant pHluorin developed to overexpress, purify and crystallize the C-terminal domain of HsdR are reported. The shortest of the three encompassed HsdR residues 887-1038 and yielded crystals that belonged to the orthorhombic space group C2221, with unit-cell parameters a = 83.42, b = 176.58, c = 126.03 Å, α = β = γ = 90.00° and two molecules in the asymmetric unit (VM = 2.55 Å(3) Da(-1), solvent content 50.47%). X-ray diffraction data were collected to a resolution of 2.45 Å.

• Klíčová slova
• EcoR124I, Escherichia coli, GFP, HsdR, pHluorin, restriction-modification system,
• MeSH
• difrakce rentgenového záření MeSH
• Escherichia coli chemie   enzymologie   genetika   MeSH
• exprese genu MeSH
• klonování DNA MeSH
• krystalizace MeSH
• krystalografie rentgenová MeSH
• plazmidy chemie   metabolismus   MeSH
• podjednotky proteinů chemie   genetika   metabolismus   MeSH
• proteiny z Escherichia coli chemie   genetika   metabolismus   MeSH
• rekombinantní fúzní proteiny chemie   genetika   metabolismus   MeSH
• restrikční endonukleasy typu I chemie   genetika   metabolismus   MeSH
• sekvence aminokyselin MeSH
• zelené fluorescenční proteiny chemie   genetika   metabolismus   MeSH
• Publikační typ
• časopisecké články MeSH
• Názvy látek
• endodeoxyribonuclease EcoR124I MeSH Prohlížeč
• HsdR protein, E coli MeSH Prohlížeč
• PHluorin MeSH Prohlížeč
• podjednotky proteinů MeSH
• proteiny z Escherichia coli MeSH
• rekombinantní fúzní proteiny MeSH
• restrikční endonukleasy typu I MeSH
• zelené fluorescenční proteiny MeSH

Type I restriction-modification enzymes are multifunctional heteromeric complexes with DNA cleavage and ATP-dependent DNA translocation activities located on motor subunit HsdR. Functional coupling of DNA cleavage and translocation is a hallmark of the Type I restriction systems that is consistent with their proposed role in horizontal gene transfer. DNA cleavage occurs at nonspecific sites distant from the cognate recognition sequence, apparently triggered by stalled translocation. The X-ray crystal structure of the complete HsdR subunit from E. coli plasmid R124 suggested that the triggering mechanism involves interdomain contacts mediated by ATP. In the present work, in vivo and in vitro activity assays and crystal structures of three mutants of EcoR124I HsdR designed to probe this mechanism are reported. The results indicate that interdomain engagement via ATP is indeed responsible for signal transmission between the endonuclease and helicase domains of the motor subunit. A previously identified sequence motif that is shared by the RecB nucleases and some Type I endonucleases is implicated in signaling.

• MeSH
• adenosintrifosfát chemie   metabolismus   MeSH
• DNA bakterií MeSH
• Escherichia coli genetika   metabolismus   MeSH
• exodeoxyribonukleasa V chemie   genetika   metabolismus   MeSH
• exprese genu MeSH
• konformace nukleové kyseliny MeSH
• krystalografie rentgenová MeSH
• molekulární modely MeSH
• mutace MeSH
• plazmidy chemie   metabolismus   MeSH
• podjednotky proteinů chemie   genetika   metabolismus   MeSH
• proteiny - lokalizační signály MeSH
• proteiny z Escherichia coli chemie   genetika   metabolismus   MeSH
• restrikční endonukleasy typu I chemie   genetika   metabolismus   MeSH
• signální transdukce MeSH
• štěpení DNA MeSH
• terciární struktura proteinů MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Názvy látek
• adenosintrifosfát MeSH
• DNA bakterií MeSH
• exodeoxyribonuclease V, E coli MeSH Prohlížeč
• exodeoxyribonukleasa V MeSH
• HsdR protein, E coli MeSH Prohlížeč
• podjednotky proteinů MeSH
• proteiny - lokalizační signály MeSH
• proteiny z Escherichia coli MeSH
• restrikční endonukleasy typu I MeSH

Restriction-modification systems protect bacteria from foreign DNA. Type I restriction-modification enzymes are multifunctional heteromeric complexes with DNA-cleavage and ATP-dependent DNA translocation activities located on endonuclease/motor subunit HsdR. The recent structure of the first intact motor subunit of the type I restriction enzyme from plasmid EcoR124I suggested a mechanism by which stalled translocation triggers DNA cleavage via a lysine residue on the endonuclease domain that contacts ATP bound between the two helicase domains. In the present work, molecular dynamics simulations are used to explore this proposal. Molecular dynamics simulations suggest that the Lys-ATP contact alternates with a contact with a nearby loop housing the conserved QxxxY motif that had been implicated in DNA cleavage. This model is tested here using in vivo and in vitro experiments. The results indicate how local interactions are transduced to domain motions within the endonuclease/motor subunit.

• MeSH
• adenosintrifosfát chemie   metabolismus   MeSH
• aminokyselinové motivy MeSH
• DNA chemie   metabolismus   MeSH
• fenotyp MeSH
• genotyp MeSH
• hydrolýza MeSH
• katalýza MeSH
• kinetika MeSH
• konzervovaná sekvence MeSH
• kvantová teorie MeSH
• lysin MeSH
• mutace MeSH
• mutageneze cílená MeSH
• restrikční endonukleasy typu I chemie   genetika   metabolismus   MeSH
• simulace molekulární dynamiky MeSH
• terciární struktura proteinů MeSH
• vazba proteinů MeSH
• vazebná místa MeSH
• vztahy mezi strukturou a aktivitou MeSH
• Publikační typ
• časopisecké články MeSH
• práce podpořená grantem MeSH
• Research Support, U.S. Gov't, Non-P.H.S. MeSH
• Názvy látek
• adenosintrifosfát MeSH
• DNA MeSH
• endodeoxyribonuclease EcoR124I MeSH Prohlížeč
• lysin MeSH
• restrikční endonukleasy typu I MeSH