The ever-growing field of microfluidics requires precise and flexible control over fluid flows at reduced scales. Current constraints demand a variety of controllable components to carry out several operations inside microchambers and microreactors. In this context, brand-new nanophotonic approaches can significantly enhance existing capabilities providing unique functionalities via finely tuned light-matter interactions. A concept is proposed, featuring dual on-chip functionality: boosted optically driven diffusion and nanoparticle sorting. High-index dielectric nanoantennae is specially designed to ensure strongly enhanced spin-orbit angular momentum transfer from a laser beam to the scattered field. Hence, subwavelength optical nanovortices emerge driving spiral motion of plasmonic nanoparticles via the interplay between curl-spin optical forces and radiation pressure. The nanovortex size is an order of magnitude smaller than that provided by conventional beam-based approaches. The nanoparticles mediate nanoconfined fluid motion enabling moving-part-free nanomixing inside a microchamber. Moreover, exploiting the nontrivial size dependence of the curled optical forces makes it possible to achieve precise nanoscale sorting of gold nanoparticles, demanded for on-chip separation and filtering. Altogether, a versatile platform is introduced for further miniaturization of moving-part-free, optically driven microfluidic chips for fast chemical analysis, emulsion preparation, or chemical gradient generation with light-controlled navigation of nanoparticles, viruses or biomolecules.

• Keywords
• all‐dielectric nanophotonics, lab‐on‐a‐chip platforms, nanofluidics, optomechanical manipulations, spin‐orbit couplings,
• Publication type
• Journal Article MeSH

Analyzing the cells in various body fluids can greatly deepen the understanding of the mechanisms governing the cellular physiology. Due to the variability of physiological and metabolic states, it is important to be able to perform such studies on individual cells. Therefore, we developed an optofluidic system in which we precisely manipulated and monitored individual cells of Escherichia coli. We tested optical micromanipulation in a microfluidic chamber chip by transferring individual bacteria into the chambers. We then subjected the cells in the chambers to antibiotic cefotaxime and we observed the changes by using time-lapse microscopy. Separately, we used laser tweezers Raman spectroscopy (LTRS) in a different micro-chamber chip to manipulate and analyze individual cefotaxime-treated E. coli cells. Additionally, we performed conventional Raman micro-spectroscopic measurements of E. coli cells in a micro-chamber. We found observable changes in the cellular morphology (cell elongation) and in Raman spectra, which were consistent with other recently published observations. The principal component analysis (PCA) of Raman data distinguished between the cefotaxime treated cells and control. We tested the capabilities of the optofluidic system and found it to be a reliable and versatile solution for this class of microbiological experiments.

• Keywords
• E. coli, Raman micro-spectroscopy, antibiotics, optical tweezers, opto-fluidics,
• MeSH
• Principal Component Analysis MeSH
• Anti-Bacterial Agents adverse effects   MeSH
• Escherichia coli drug effects   growth & development   MeSH
• Lab-On-A-Chip Devices * MeSH
• Micromanipulation methods   MeSH
• Optical Tweezers * MeSH
• Spectrum Analysis, Raman MeSH
• Publication type
• Journal Article MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH

It was previously believed that larger metal nanoparticles behave as tiny mirrors that are pushed by the light beam radiative force along the direction of beam propagation, without a chance to be confined. However, several groups have recently reported successful optical trapping of gold and silver particles as large as 250 nm. We offer a possible explanation based on the fact that metal nanoparticles naturally occur in various non-spherical shapes and their optical properties differ significantly due to changes in localized plasmon excitation. We demonstrate experimentally and support theoretically three-dimensional confinement of large gold nanoparticles in an optical trap based on very low numerical aperture optics. We showed theoretically that the unique properties of gold nanoprisms allow an increase of trapping force by an order of magnitude at certain aspect ratios. These results pave the way to spatial manipulation of plasmonic nanoparticles using an optical fibre, with interesting applications in biology and medicine.

• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH

Algal biomass that is represented mainly by commercially grown algal strains has recently found many potential applications in various fields of interest. Its utilization has been found advantageous in the fields of bioremediation, biofuel production and the food industry. This paper reviews recent developments in the analysis of algal biomass with the main focus on the Laser-Induced Breakdown Spectroscopy, Raman spectroscopy, and partly Laser-Ablation Inductively Coupled Plasma techniques. The advantages of the selected laser-based analytical techniques are revealed and their fields of use are discussed in detail.

• MeSH
• Equipment Failure Analysis MeSH
• Biomass * MeSH
• Equipment Design MeSH
• Eukaryota cytology   growth & development   MeSH
• Lasers * MeSH
• Water Microbiology * MeSH
• Environmental Monitoring instrumentation   MeSH
• Spectrum Analysis instrumentation   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Review MeSH

Antibiotics cure infections by influencing bacterial growth or viability. Antibiotics can be divided to two groups on the basis of their effect on microbial cells through two main mechanisms, which are either bactericidal or bacteriostatic. Bactericidal antibiotics kill the bacteria and bacteriostatic antibiotics suppress the growth of bacteria (keep them in the stationary phase of growth). One of many factors to predict a favorable clinical outcome of the potential action of antimicrobial chemicals may be provided using in vitro bactericidal/bacteriostatic data (e.g., minimum inhibitory concentrations-MICs). Consequently, MICs are used in clinical situations mainly to confirm resistance, and to determine the in vitro activities of new antimicrobials. We report on the combination of data obtained from MICs with information on microorganisms' "fingerprint" (e.g., DNA/RNA, and proteins) provided by Raman spectroscopy. Thus, we could follow mechanisms of the bacteriostatic versus bactericidal action simply by detecting the Raman bands corresponding to DNA. The Raman spectra of Staphylococcus epidermidis treated with clindamycin (a bacteriostatic agent) indeed show little effect on DNA which is in contrast with the action of ciprofloxacin (a bactericidal agent), where the Raman spectra show a decrease in strength of the signal assigned to DNA, suggesting DNA fragmentation.

• MeSH
• Anti-Bacterial Agents pharmacology   MeSH
• Ciprofloxacin pharmacology   MeSH
• DNA, Bacterial drug effects   MeSH
• Clindamycin pharmacology   MeSH
• Microbial Sensitivity Tests MeSH
• Spectrum Analysis, Raman methods   MeSH
• Staphylococcus epidermidis drug effects   genetics   MeSH
• Publication type
• Journal Article MeSH
• Research Support, Non-U.S. Gov't MeSH
• Names of Substances
• Anti-Bacterial Agents MeSH
• Ciprofloxacin MeSH
• DNA, Bacterial MeSH
• Clindamycin MeSH